Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6728 and GFF6729 are separated by 33 nucleotides GFF6729 and GFF6730 overlap by 4 nucleotides GFF6730 and GFF6731 overlap by 8 nucleotides
GFF6728 - Probable VANILLIN dehydrogenase oxidoreductase protein (EC 1.-.-.-), at 38,557 to 40,008
GFF6728
GFF6729 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 40,042 to 40,755
GFF6729
GFF6730 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 40,752 to 41,504
GFF6730
GFF6731 - Nucleoside ABC transporter, permease protein 1, at 41,497 to 42,435
GFF6731
Position (kb) 40
41 Strain fitness (log2 ratio) -2
-1
0
1 at 39.278 kb on + strand, within GFF6728 at 39.580 kb on + strand, within GFF6728 at 39.580 kb on + strand, within GFF6728 at 39.583 kb on + strand, within GFF6728 at 39.584 kb on - strand, within GFF6728 at 40.136 kb on + strand, within GFF6729 at 40.709 kb on + strand at 40.876 kb on + strand, within GFF6730 at 40.876 kb on + strand, within GFF6730 at 40.877 kb on - strand, within GFF6730 at 40.877 kb on - strand, within GFF6730 at 41.267 kb on - strand, within GFF6730 at 41.267 kb on - strand, within GFF6730 at 41.267 kb on - strand, within GFF6730 at 41.267 kb on - strand, within GFF6730 at 41.567 kb on + strand at 41.610 kb on - strand, within GFF6731 at 41.610 kb on - strand, within GFF6731
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 39,278 + GFF6728 0.50 -0.1 39,580 + GFF6728 0.70 +1.3 39,580 + GFF6728 0.70 +0.3 39,583 + GFF6728 0.71 -0.1 39,584 - GFF6728 0.71 +0.4 40,136 + GFF6729 0.13 -0.7 40,709 + -2.5 40,876 + GFF6730 0.16 -0.5 40,876 + GFF6730 0.16 -0.1 40,877 - GFF6730 0.17 +0.2 40,877 - GFF6730 0.17 -0.7 41,267 - GFF6730 0.68 -0.2 41,267 - GFF6730 0.68 +0.3 41,267 - GFF6730 0.68 -0.5 41,267 - GFF6730 0.68 +0.5 41,567 + -0.9 41,610 - GFF6731 0.12 -0.9 41,610 - GFF6731 0.12 +0.6
Or see this region's nucleotide sequence