Strain Fitness in Variovorax sp. SCN45 around GFF6702

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6701 and GFF6702 are separated by 55 nucleotidesGFF6702 and GFF6703 overlap by 8 nucleotidesGFF6703 and GFF6704 are separated by 4 nucleotides GFF6701 - Regulatory protein, RpfE type, at 9,981 to 10,949 GFF6701 GFF6702 - Lipoprotein releasing system transmembrane protein LolC/LolE, at 11,005 to 12,261 GFF6702 GFF6703 - Lipoprotein-releasing system ATP-binding protein LolD, at 12,254 to 12,931 GFF6703 GFF6704 - no description, at 12,936 to 13,151 GFF6704 Position (kb) 11 12 13Strain fitness (log2 ratio) -2 -1 0 1 2at 10.066 kb on + strandat 10.066 kb on + strandat 10.067 kb on - strandat 10.067 kb on - strandat 10.123 kb on + strand, within GFF6701at 10.351 kb on + strand, within GFF6701at 10.352 kb on - strand, within GFF6701at 10.352 kb on - strand, within GFF6701at 10.955 kb on + strandat 10.956 kb on - strandat 11.004 kb on + strandat 11.004 kb on + strandat 12.268 kb on - strandat 12.268 kb on - strandat 12.268 kb on - strandat 12.268 kb on - strandat 13.022 kb on + strand, within GFF6704at 13.023 kb on - strand, within GFF6704at 13.023 kb on - strand, within GFF6704at 13.120 kb on + strand, within GFF6704at 13.120 kb on + strand, within GFF6704

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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10,066 + +1.0
10,066 + -0.0
10,067 - +0.5
10,067 - +0.0
10,123 + GFF6701 0.15 +1.2
10,351 + GFF6701 0.38 +0.6
10,352 - GFF6701 0.38 -0.3
10,352 - GFF6701 0.38 -1.9
10,955 + +2.6
10,956 - -1.4
11,004 + +0.0
11,004 + +0.2
12,268 - +0.2
12,268 - +0.3
12,268 - -0.2
12,268 - -0.4
13,022 + GFF6704 0.40 -0.6
13,023 - GFF6704 0.40 -0.2
13,023 - GFF6704 0.40 +0.6
13,120 + GFF6704 0.85 +0.7
13,120 + GFF6704 0.85 -0.1

Or see this region's nucleotide sequence