Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6651 and GFF6652 are separated by 13 nucleotides GFF6652 and GFF6653 are separated by 53 nucleotides GFF6653 and GFF6654 are separated by 144 nucleotides
GFF6651 - Asp/Glu racemase, at 24,983 to 25,624
GFF6651
GFF6652 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 25,638 to 26,552
GFF6652
GFF6653 - Aminotransferase, class III, at 26,606 to 27,940
GFF6653
GFF6654 - Transcriptional regulator, RpiR family, at 28,085 to 28,951
GFF6654
Position (kb) 26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 25.696 kb on + strand at 25.697 kb on - strand at 25.748 kb on - strand, within GFF6652 at 25.748 kb on - strand, within GFF6652 at 26.146 kb on + strand, within GFF6652 at 26.146 kb on + strand, within GFF6652 at 26.146 kb on + strand, within GFF6652 at 26.147 kb on - strand, within GFF6652 at 26.275 kb on + strand, within GFF6652 at 26.275 kb on + strand, within GFF6652 at 26.275 kb on + strand, within GFF6652 at 26.275 kb on + strand, within GFF6652 at 26.276 kb on - strand, within GFF6652 at 26.276 kb on - strand, within GFF6652 at 26.276 kb on - strand, within GFF6652 at 26.276 kb on - strand, within GFF6652 at 26.351 kb on - strand, within GFF6652 at 26.351 kb on - strand, within GFF6652 at 26.380 kb on + strand, within GFF6652 at 26.381 kb on - strand, within GFF6652 at 26.407 kb on + strand, within GFF6652 at 26.752 kb on - strand, within GFF6653 at 26.752 kb on - strand, within GFF6653 at 26.752 kb on - strand, within GFF6653 at 27.030 kb on + strand, within GFF6653 at 27.030 kb on + strand, within GFF6653 at 27.030 kb on + strand, within GFF6653 at 27.031 kb on - strand, within GFF6653 at 27.031 kb on - strand, within GFF6653 at 27.031 kb on - strand, within GFF6653 at 27.031 kb on - strand, within GFF6653 at 27.031 kb on - strand, within GFF6653 at 27.031 kb on - strand, within GFF6653 at 27.031 kb on - strand, within GFF6653 at 27.243 kb on + strand, within GFF6653 at 27.243 kb on + strand, within GFF6653 at 27.243 kb on + strand, within GFF6653 at 27.243 kb on + strand, within GFF6653 at 27.243 kb on + strand, within GFF6653 at 27.243 kb on + strand, within GFF6653 at 27.243 kb on + strand, within GFF6653 at 27.244 kb on - strand, within GFF6653 at 27.244 kb on - strand, within GFF6653 at 27.244 kb on - strand, within GFF6653 at 27.244 kb on - strand, within GFF6653 at 27.276 kb on + strand, within GFF6653 at 27.276 kb on + strand, within GFF6653 at 27.277 kb on - strand, within GFF6653 at 27.403 kb on - strand, within GFF6653 at 27.444 kb on + strand, within GFF6653 at 27.445 kb on - strand, within GFF6653 at 27.445 kb on - strand, within GFF6653 at 27.445 kb on - strand, within GFF6653 at 27.546 kb on + strand, within GFF6653 at 27.546 kb on + strand, within GFF6653 at 27.546 kb on + strand, within GFF6653 at 27.546 kb on + strand, within GFF6653 at 27.546 kb on + strand, within GFF6653 at 27.547 kb on - strand, within GFF6653 at 27.547 kb on - strand, within GFF6653 at 27.547 kb on - strand, within GFF6653 at 27.547 kb on - strand, within GFF6653 at 27.603 kb on + strand, within GFF6653 at 27.603 kb on + strand, within GFF6653 at 27.603 kb on + strand, within GFF6653 at 27.604 kb on - strand, within GFF6653 at 27.604 kb on - strand, within GFF6653 at 27.604 kb on - strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.660 kb on + strand, within GFF6653 at 27.661 kb on - strand, within GFF6653 at 27.661 kb on - strand, within GFF6653 at 27.661 kb on - strand, within GFF6653 at 27.661 kb on - strand, within GFF6653 at 27.661 kb on - strand, within GFF6653 at 27.661 kb on - strand, within GFF6653 at 27.750 kb on + strand, within GFF6653 at 27.750 kb on + strand, within GFF6653 at 27.750 kb on + strand, within GFF6653 at 27.750 kb on + strand, within GFF6653 at 27.750 kb on + strand, within GFF6653 at 27.750 kb on + strand, within GFF6653 at 27.751 kb on - strand, within GFF6653 at 27.751 kb on - strand, within GFF6653 at 27.751 kb on - strand, within GFF6653 at 27.751 kb on - strand, within GFF6653 at 27.846 kb on + strand at 27.847 kb on - strand at 27.847 kb on - strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.873 kb on + strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 27.874 kb on - strand at 28.154 kb on + strand at 28.154 kb on + strand at 28.154 kb on + strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.155 kb on - strand at 28.325 kb on + strand, within GFF6654 at 28.325 kb on + strand, within GFF6654 at 28.325 kb on + strand, within GFF6654 at 28.325 kb on + strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.326 kb on - strand, within GFF6654 at 28.464 kb on + strand, within GFF6654 at 28.465 kb on - strand, within GFF6654 at 28.465 kb on - strand, within GFF6654 at 28.465 kb on - strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.532 kb on + strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.533 kb on - strand, within GFF6654 at 28.538 kb on + strand, within GFF6654 at 28.538 kb on + strand, within GFF6654 at 28.538 kb on + strand, within GFF6654 at 28.539 kb on - strand, within GFF6654 at 28.539 kb on - strand, within GFF6654 at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.919 kb on + strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand at 28.920 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 25,696 + +0.6 25,697 - -0.8 25,748 - GFF6652 0.12 -0.1 25,748 - GFF6652 0.12 +1.3 26,146 + GFF6652 0.56 +0.0 26,146 + GFF6652 0.56 +0.5 26,146 + GFF6652 0.56 -0.1 26,147 - GFF6652 0.56 -0.6 26,275 + GFF6652 0.70 -0.2 26,275 + GFF6652 0.70 +0.1 26,275 + GFF6652 0.70 +0.4 26,275 + GFF6652 0.70 -1.0 26,276 - GFF6652 0.70 -0.4 26,276 - GFF6652 0.70 -0.9 26,276 - GFF6652 0.70 -1.7 26,276 - GFF6652 0.70 -0.4 26,351 - GFF6652 0.78 +0.0 26,351 - GFF6652 0.78 -0.2 26,380 + GFF6652 0.81 -0.4 26,381 - GFF6652 0.81 +0.0 26,407 + GFF6652 0.84 -0.3 26,752 - GFF6653 0.11 +0.8 26,752 - GFF6653 0.11 -1.7 26,752 - GFF6653 0.11 -1.3 27,030 + GFF6653 0.32 +0.3 27,030 + GFF6653 0.32 +2.1 27,030 + GFF6653 0.32 +0.3 27,031 - GFF6653 0.32 +0.3 27,031 - GFF6653 0.32 -0.6 27,031 - GFF6653 0.32 -0.2 27,031 - GFF6653 0.32 +0.2 27,031 - GFF6653 0.32 -0.3 27,031 - GFF6653 0.32 -0.7 27,031 - GFF6653 0.32 -0.1 27,243 + GFF6653 0.48 +1.6 27,243 + GFF6653 0.48 +0.1 27,243 + GFF6653 0.48 +0.5 27,243 + GFF6653 0.48 +0.5 27,243 + GFF6653 0.48 -0.1 27,243 + GFF6653 0.48 -0.0 27,243 + GFF6653 0.48 -1.1 27,244 - GFF6653 0.48 -0.6 27,244 - GFF6653 0.48 -0.6 27,244 - GFF6653 0.48 -1.7 27,244 - GFF6653 0.48 -1.5 27,276 + GFF6653 0.50 -0.0 27,276 + GFF6653 0.50 +1.2 27,277 - GFF6653 0.50 +0.0 27,403 - GFF6653 0.60 -0.0 27,444 + GFF6653 0.63 +0.1 27,445 - GFF6653 0.63 +0.3 27,445 - GFF6653 0.63 +1.0 27,445 - GFF6653 0.63 -1.1 27,546 + GFF6653 0.70 -0.2 27,546 + GFF6653 0.70 -0.1 27,546 + GFF6653 0.70 +0.3 27,546 + GFF6653 0.70 -0.6 27,546 + GFF6653 0.70 +0.0 27,547 - GFF6653 0.70 +1.2 27,547 - GFF6653 0.70 -0.2 27,547 - GFF6653 0.70 -1.0 27,547 - GFF6653 0.70 -1.7 27,603 + GFF6653 0.75 -0.3 27,603 + GFF6653 0.75 -0.0 27,603 + GFF6653 0.75 +0.7 27,604 - GFF6653 0.75 -0.2 27,604 - GFF6653 0.75 -1.0 27,604 - GFF6653 0.75 +0.2 27,660 + GFF6653 0.79 -0.5 27,660 + GFF6653 0.79 +1.2 27,660 + GFF6653 0.79 +0.3 27,660 + GFF6653 0.79 +0.6 27,660 + GFF6653 0.79 -0.5 27,660 + GFF6653 0.79 +0.1 27,660 + GFF6653 0.79 -0.8 27,660 + GFF6653 0.79 -0.2 27,660 + GFF6653 0.79 -1.8 27,660 + GFF6653 0.79 -0.3 27,661 - GFF6653 0.79 +0.3 27,661 - GFF6653 0.79 +0.9 27,661 - GFF6653 0.79 +0.0 27,661 - GFF6653 0.79 -1.4 27,661 - GFF6653 0.79 +0.2 27,661 - GFF6653 0.79 +1.7 27,750 + GFF6653 0.86 -0.1 27,750 + GFF6653 0.86 -0.2 27,750 + GFF6653 0.86 +0.1 27,750 + GFF6653 0.86 +0.1 27,750 + GFF6653 0.86 +0.3 27,750 + GFF6653 0.86 -1.0 27,751 - GFF6653 0.86 +0.4 27,751 - GFF6653 0.86 -1.2 27,751 - GFF6653 0.86 -0.7 27,751 - GFF6653 0.86 -1.4 27,846 + -0.3 27,847 - -0.3 27,847 - +0.2 27,873 + +0.3 27,873 + -0.2 27,873 + +1.3 27,873 + +0.6 27,873 + -0.5 27,873 + -0.1 27,873 + +2.3 27,873 + +0.1 27,873 + -0.5 27,873 + -0.8 27,873 + -0.0 27,873 + -0.2 27,873 + -0.2 27,873 + +0.1 27,873 + -0.5 27,873 + -0.4 27,873 + -0.2 27,873 + -0.1 27,873 + -0.0 27,873 + -0.4 27,874 - -0.3 27,874 - -0.7 27,874 - -0.6 27,874 - +0.1 27,874 - -0.2 27,874 - -0.6 27,874 - -0.5 27,874 - -0.2 27,874 - -1.7 27,874 - -0.8 27,874 - +0.6 27,874 - -1.8 27,874 - -0.5 27,874 - -0.5 27,874 - -0.1 27,874 - -1.5 27,874 - +0.3 27,874 - +0.3 27,874 - +0.6 27,874 - +0.2 27,874 - +0.4 27,874 - +0.1 27,874 - +0.8 27,874 - +0.8 27,874 - -0.5 27,874 - +1.7 28,154 + +0.7 28,154 + +0.6 28,154 + +0.5 28,155 - -0.4 28,155 - -2.1 28,155 - +1.1 28,155 - -2.2 28,155 - +0.6 28,155 - -0.7 28,325 + GFF6654 0.28 -0.2 28,325 + GFF6654 0.28 -0.1 28,325 + GFF6654 0.28 -0.5 28,325 + GFF6654 0.28 +0.8 28,326 - GFF6654 0.28 +0.0 28,326 - GFF6654 0.28 -0.2 28,326 - GFF6654 0.28 -0.4 28,326 - GFF6654 0.28 -0.2 28,326 - GFF6654 0.28 +0.3 28,326 - GFF6654 0.28 -0.4 28,326 - GFF6654 0.28 +0.3 28,326 - GFF6654 0.28 -0.8 28,326 - GFF6654 0.28 -0.1 28,326 - GFF6654 0.28 -1.2 28,326 - GFF6654 0.28 -0.1 28,326 - GFF6654 0.28 +1.1 28,326 - GFF6654 0.28 -1.2 28,464 + GFF6654 0.44 +0.2 28,465 - GFF6654 0.44 +0.3 28,465 - GFF6654 0.44 -0.4 28,465 - GFF6654 0.44 +1.9 28,532 + GFF6654 0.52 -0.4 28,532 + GFF6654 0.52 -1.0 28,532 + GFF6654 0.52 +0.3 28,532 + GFF6654 0.52 +0.2 28,532 + GFF6654 0.52 +0.2 28,532 + GFF6654 0.52 +1.1 28,532 + GFF6654 0.52 -0.5 28,532 + GFF6654 0.52 -0.2 28,532 + GFF6654 0.52 -0.2 28,532 + GFF6654 0.52 -1.1 28,532 + GFF6654 0.52 +0.6 28,532 + GFF6654 0.52 +0.3 28,532 + GFF6654 0.52 -0.1 28,533 - GFF6654 0.52 +0.2 28,533 - GFF6654 0.52 +0.4 28,533 - GFF6654 0.52 +1.3 28,533 - GFF6654 0.52 +0.3 28,533 - GFF6654 0.52 -0.1 28,533 - GFF6654 0.52 +0.3 28,533 - GFF6654 0.52 +0.9 28,533 - GFF6654 0.52 -0.2 28,533 - GFF6654 0.52 +0.1 28,533 - GFF6654 0.52 +0.8 28,538 + GFF6654 0.52 +0.3 28,538 + GFF6654 0.52 +0.8 28,538 + GFF6654 0.52 -0.1 28,539 - GFF6654 0.52 -0.6 28,539 - GFF6654 0.52 -0.9 28,919 + -1.0 28,919 + -0.2 28,919 + -1.8 28,919 + +0.3 28,919 + -3.2 28,919 + -0.0 28,919 + +1.8 28,919 + -2.6 28,920 - -0.1 28,920 - -0.7 28,920 - -0.3 28,920 - +0.6 28,920 - -0.8 28,920 - -0.7 28,920 - -0.1 28,920 - +0.9 28,920 - +0.8 28,920 - +0.2 28,920 - -0.1
Or see this region's nucleotide sequence