Strain Fitness in Variovorax sp. SCN45 around GFF659

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF658 and GFF659 are separated by 34 nucleotidesGFF659 and GFF660 are separated by 250 nucleotides GFF658 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 709,630 to 710,646 GFF658 GFF659 - Alanyl-tRNA synthetase (EC 6.1.1.7), at 710,681 to 713,305 GFF659 GFF660 - FIG00974024: hypothetical protein, at 713,556 to 714,377 GFF660 Position (kb) 710 711 712 713 714Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 709.789 kb on - strand, within GFF658at 710.240 kb on + strand, within GFF658at 710.240 kb on + strand, within GFF658at 710.240 kb on + strand, within GFF658at 710.240 kb on + strand, within GFF658at 710.241 kb on - strand, within GFF658at 710.241 kb on - strand, within GFF658at 710.241 kb on - strand, within GFF658at 710.241 kb on - strand, within GFF658at 710.241 kb on - strand, within GFF658at 710.241 kb on - strand, within GFF658at 713.412 kb on + strandat 713.412 kb on + strandat 713.412 kb on + strandat 713.412 kb on + strandat 713.412 kb on + strandat 713.413 kb on - strandat 713.413 kb on - strandat 713.413 kb on - strandat 713.475 kb on - strandat 713.475 kb on - strandat 713.475 kb on - strandat 713.475 kb on - strandat 713.477 kb on + strandat 713.477 kb on + strandat 713.478 kb on - strandat 713.484 kb on + strandat 713.485 kb on - strandat 713.525 kb on + strandat 713.525 kb on + strandat 713.525 kb on + strandat 713.525 kb on + strandat 713.525 kb on + strandat 713.525 kb on + strandat 713.525 kb on + strandat 713.525 kb on + strandat 713.526 kb on - strandat 713.526 kb on - strandat 713.526 kb on - strandat 713.531 kb on + strandat 713.531 kb on + strandat 713.531 kb on + strandat 713.532 kb on - strandat 713.596 kb on + strandat 713.649 kb on + strand, within GFF660at 713.649 kb on + strand, within GFF660at 713.649 kb on + strand, within GFF660at 713.649 kb on + strand, within GFF660at 713.649 kb on + strand, within GFF660at 713.649 kb on + strand, within GFF660at 713.649 kb on + strand, within GFF660at 713.649 kb on + strand, within GFF660at 713.650 kb on - strand, within GFF660at 713.650 kb on - strand, within GFF660at 713.650 kb on - strand, within GFF660at 713.650 kb on - strand, within GFF660at 713.650 kb on - strand, within GFF660at 713.694 kb on + strand, within GFF660at 713.694 kb on + strand, within GFF660at 713.694 kb on + strand, within GFF660at 713.694 kb on + strand, within GFF660at 713.694 kb on + strand, within GFF660at 713.695 kb on - strand, within GFF660at 713.695 kb on - strand, within GFF660at 713.695 kb on - strand, within GFF660at 713.802 kb on + strand, within GFF660at 713.802 kb on + strand, within GFF660at 713.802 kb on + strand, within GFF660at 713.802 kb on + strand, within GFF660at 713.803 kb on - strand, within GFF660at 713.803 kb on - strand, within GFF660at 713.803 kb on - strand, within GFF660at 713.866 kb on - strand, within GFF660at 713.866 kb on - strand, within GFF660at 713.866 kb on - strand, within GFF660at 713.866 kb on - strand, within GFF660at 713.866 kb on - strand, within GFF660at 713.874 kb on + strand, within GFF660at 713.874 kb on + strand, within GFF660at 713.874 kb on + strand, within GFF660at 713.874 kb on + strand, within GFF660at 713.874 kb on + strand, within GFF660at 713.874 kb on + strand, within GFF660at 713.875 kb on - strand, within GFF660at 713.875 kb on - strand, within GFF660at 713.875 kb on - strand, within GFF660at 713.875 kb on - strand, within GFF660at 713.875 kb on - strand, within GFF660at 713.955 kb on + strand, within GFF660at 713.955 kb on + strand, within GFF660at 713.955 kb on + strand, within GFF660at 713.955 kb on + strand, within GFF660at 713.955 kb on + strand, within GFF660at 713.955 kb on + strand, within GFF660at 713.956 kb on - strand, within GFF660at 713.956 kb on - strand, within GFF660at 713.956 kb on - strand, within GFF660at 713.956 kb on - strand, within GFF660at 713.956 kb on - strand, within GFF660at 713.956 kb on - strand, within GFF660at 714.039 kb on + strand, within GFF660at 714.039 kb on + strand, within GFF660at 714.039 kb on + strand, within GFF660at 714.039 kb on + strand, within GFF660at 714.039 kb on + strand, within GFF660at 714.040 kb on - strand, within GFF660at 714.040 kb on - strand, within GFF660at 714.040 kb on - strand, within GFF660at 714.040 kb on - strand, within GFF660at 714.040 kb on - strand, within GFF660at 714.040 kb on - strand, within GFF660at 714.040 kb on - strand, within GFF660at 714.051 kb on + strand, within GFF660at 714.051 kb on + strand, within GFF660at 714.052 kb on - strand, within GFF660at 714.052 kb on - strand, within GFF660at 714.052 kb on - strand, within GFF660at 714.052 kb on - strand, within GFF660at 714.213 kb on + strand, within GFF660at 714.213 kb on + strand, within GFF660at 714.213 kb on + strand, within GFF660at 714.213 kb on + strand, within GFF660at 714.214 kb on - strand, within GFF660at 714.214 kb on - strand, within GFF660at 714.214 kb on - strand, within GFF660at 714.214 kb on - strand, within GFF660at 714.214 kb on - strand, within GFF660at 714.214 kb on - strand, within GFF660at 714.214 kb on - strand, within GFF660at 714.214 kb on - strand, within GFF660

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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709,789 - GFF658 0.16 -0.6
710,240 + GFF658 0.60 +0.2
710,240 + GFF658 0.60 +0.4
710,240 + GFF658 0.60 -0.0
710,240 + GFF658 0.60 -0.4
710,241 - GFF658 0.60 -0.0
710,241 - GFF658 0.60 +1.0
710,241 - GFF658 0.60 -0.4
710,241 - GFF658 0.60 +0.6
710,241 - GFF658 0.60 -0.5
710,241 - GFF658 0.60 +0.1
713,412 + -0.3
713,412 + +1.9
713,412 + -0.1
713,412 + +0.2
713,412 + -0.0
713,413 - -0.6
713,413 - -0.2
713,413 - -1.3
713,475 - -0.4
713,475 - -0.6
713,475 - -0.6
713,475 - -0.3
713,477 + -0.9
713,477 + -1.5
713,478 - +3.0
713,484 + -1.3
713,485 - -0.1
713,525 + -1.5
713,525 + -0.3
713,525 + -1.0
713,525 + -2.3
713,525 + +0.7
713,525 + -0.5
713,525 + -0.0
713,525 + +0.3
713,526 - -0.4
713,526 - -0.3
713,526 - -0.4
713,531 + -0.7
713,531 + -0.2
713,531 + +0.0
713,532 - -1.4
713,596 + -1.1
713,649 + GFF660 0.11 +0.1
713,649 + GFF660 0.11 -0.1
713,649 + GFF660 0.11 -0.0
713,649 + GFF660 0.11 -0.8
713,649 + GFF660 0.11 +0.2
713,649 + GFF660 0.11 +1.5
713,649 + GFF660 0.11 -0.6
713,649 + GFF660 0.11 -0.3
713,650 - GFF660 0.11 -1.3
713,650 - GFF660 0.11 +0.2
713,650 - GFF660 0.11 -0.1
713,650 - GFF660 0.11 -1.1
713,650 - GFF660 0.11 -1.2
713,694 + GFF660 0.17 -1.0
713,694 + GFF660 0.17 +0.5
713,694 + GFF660 0.17 +0.1
713,694 + GFF660 0.17 +0.1
713,694 + GFF660 0.17 -1.7
713,695 - GFF660 0.17 -1.3
713,695 - GFF660 0.17 -1.1
713,695 - GFF660 0.17 -0.2
713,802 + GFF660 0.30 +1.4
713,802 + GFF660 0.30 +0.3
713,802 + GFF660 0.30 -0.1
713,802 + GFF660 0.30 +0.4
713,803 - GFF660 0.30 +1.3
713,803 - GFF660 0.30 -0.2
713,803 - GFF660 0.30 -0.7
713,866 - GFF660 0.38 +0.1
713,866 - GFF660 0.38 -1.0
713,866 - GFF660 0.38 +0.3
713,866 - GFF660 0.38 -0.1
713,866 - GFF660 0.38 +1.2
713,874 + GFF660 0.39 -0.3
713,874 + GFF660 0.39 -0.1
713,874 + GFF660 0.39 +0.9
713,874 + GFF660 0.39 -0.9
713,874 + GFF660 0.39 -0.3
713,874 + GFF660 0.39 -0.7
713,875 - GFF660 0.39 -0.4
713,875 - GFF660 0.39 +1.2
713,875 - GFF660 0.39 -0.3
713,875 - GFF660 0.39 -0.3
713,875 - GFF660 0.39 +0.5
713,955 + GFF660 0.49 -0.7
713,955 + GFF660 0.49 -0.1
713,955 + GFF660 0.49 +0.3
713,955 + GFF660 0.49 -0.5
713,955 + GFF660 0.49 -2.9
713,955 + GFF660 0.49 +0.2
713,956 - GFF660 0.49 -0.3
713,956 - GFF660 0.49 -0.4
713,956 - GFF660 0.49 -0.2
713,956 - GFF660 0.49 -0.1
713,956 - GFF660 0.49 +0.3
713,956 - GFF660 0.49 +0.7
714,039 + GFF660 0.59 +0.7
714,039 + GFF660 0.59 -0.1
714,039 + GFF660 0.59 -0.6
714,039 + GFF660 0.59 +0.1
714,039 + GFF660 0.59 -1.3
714,040 - GFF660 0.59 +1.5
714,040 - GFF660 0.59 +0.5
714,040 - GFF660 0.59 +0.3
714,040 - GFF660 0.59 -0.3
714,040 - GFF660 0.59 -0.6
714,040 - GFF660 0.59 -0.3
714,040 - GFF660 0.59 -1.3
714,051 + GFF660 0.60 +0.3
714,051 + GFF660 0.60 -1.5
714,052 - GFF660 0.60 -0.5
714,052 - GFF660 0.60 +0.7
714,052 - GFF660 0.60 +0.2
714,052 - GFF660 0.60 +1.2
714,213 + GFF660 0.80 -0.2
714,213 + GFF660 0.80 +0.9
714,213 + GFF660 0.80 +0.1
714,213 + GFF660 0.80 -0.3
714,214 - GFF660 0.80 -1.1
714,214 - GFF660 0.80 -0.2
714,214 - GFF660 0.80 -1.1
714,214 - GFF660 0.80 +0.1
714,214 - GFF660 0.80 -0.9
714,214 - GFF660 0.80 +0.1
714,214 - GFF660 0.80 -0.1
714,214 - GFF660 0.80 -0.1

Or see this region's nucleotide sequence