Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6576 and GFF6577 are separated by 12 nucleotides GFF6577 and GFF6578 overlap by 4 nucleotides GFF6578 and GFF6579 overlap by 4 nucleotides
GFF6576 - Conjugative transfer protein TrbE, at 17,046 to 19,520
GFF6576
GFF6577 - Conjugative transfer protein TrbD, at 19,533 to 19,805
GFF6577
GFF6578 - Conjugative transfer protein TrbC, at 19,802 to 20,179
GFF6578
GFF6579 - Conjugative transfer protein TrbB, at 20,176 to 21,213
GFF6579
Position (kb) 19
20
21 Strain fitness (log2 ratio) -1
0
1 at 18.873 kb on + strand, within GFF6576 at 18.964 kb on + strand, within GFF6576 at 18.965 kb on - strand, within GFF6576 at 19.147 kb on + strand, within GFF6576 at 19.148 kb on - strand, within GFF6576 at 19.165 kb on + strand, within GFF6576 at 19.165 kb on + strand, within GFF6576 at 19.165 kb on + strand, within GFF6576 at 19.165 kb on + strand, within GFF6576 at 19.165 kb on + strand, within GFF6576 at 19.166 kb on - strand, within GFF6576 at 19.166 kb on - strand, within GFF6576 at 19.166 kb on - strand, within GFF6576 at 19.166 kb on - strand, within GFF6576 at 19.166 kb on - strand, within GFF6576 at 19.271 kb on + strand, within GFF6576 at 19.535 kb on - strand at 19.535 kb on - strand at 19.806 kb on - strand at 20.156 kb on - strand at 20.825 kb on + strand, within GFF6579 at 20.825 kb on + strand, within GFF6579 at 20.825 kb on + strand, within GFF6579 at 20.825 kb on + strand, within GFF6579 at 20.826 kb on - strand, within GFF6579
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 18,873 + GFF6576 0.74 +0.7 18,964 + GFF6576 0.77 -0.1 18,965 - GFF6576 0.78 -1.0 19,147 + GFF6576 0.85 +0.1 19,148 - GFF6576 0.85 -1.7 19,165 + GFF6576 0.86 -1.1 19,165 + GFF6576 0.86 -0.6 19,165 + GFF6576 0.86 -0.5 19,165 + GFF6576 0.86 -0.3 19,165 + GFF6576 0.86 +1.2 19,166 - GFF6576 0.86 -1.2 19,166 - GFF6576 0.86 -0.5 19,166 - GFF6576 0.86 +0.4 19,166 - GFF6576 0.86 -0.4 19,166 - GFF6576 0.86 +0.0 19,271 + GFF6576 0.90 -0.2 19,535 - +0.3 19,535 - +0.0 19,806 - -0.3 20,156 - -0.9 20,825 + GFF6579 0.63 +0.4 20,825 + GFF6579 0.63 -0.6 20,825 + GFF6579 0.63 -0.3 20,825 + GFF6579 0.63 +0.4 20,826 - GFF6579 0.63 -0.0
Or see this region's nucleotide sequence