Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6530 and GFF6531 are separated by 138 nucleotides GFF6531 and GFF6532 are separated by 4 nucleotides
GFF6530 - Type I secretion outer membrane protein, TolC family, at 24,612 to 26,165
GFF6530
GFF6531 - 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12)(EC 2.4.99.13), at 26,304 to 27,689
GFF6531
GFF6532 - Phosphoglucomutase (EC 5.4.2.2) @ Phosphomannomutase (EC 5.4.2.8), at 27,694 to 29,076
GFF6532
Position (kb) 26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2 at 25.485 kb on + strand, within GFF6530 at 25.485 kb on + strand, within GFF6530 at 25.485 kb on + strand, within GFF6530 at 25.485 kb on + strand, within GFF6530 at 25.486 kb on - strand, within GFF6530 at 25.486 kb on - strand, within GFF6530 at 25.486 kb on - strand, within GFF6530 at 25.695 kb on + strand, within GFF6530 at 25.696 kb on - strand, within GFF6530 at 25.696 kb on - strand, within GFF6530 at 25.789 kb on - strand, within GFF6530 at 25.876 kb on - strand, within GFF6530 at 26.363 kb on + strand at 26.363 kb on + strand at 26.363 kb on + strand at 26.363 kb on + strand at 26.363 kb on + strand at 28.065 kb on - strand, within GFF6532 at 28.065 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.185 kb on - strand, within GFF6532 at 28.620 kb on - strand, within GFF6532 at 28.620 kb on - strand, within GFF6532 at 28.620 kb on - strand, within GFF6532 at 28.620 kb on - strand, within GFF6532
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 25,485 + GFF6530 0.56 -0.7 25,485 + GFF6530 0.56 -1.0 25,485 + GFF6530 0.56 -0.2 25,485 + GFF6530 0.56 +0.4 25,486 - GFF6530 0.56 +0.6 25,486 - GFF6530 0.56 -0.5 25,486 - GFF6530 0.56 -1.8 25,695 + GFF6530 0.70 +0.4 25,696 - GFF6530 0.70 +2.4 25,696 - GFF6530 0.70 +0.1 25,789 - GFF6530 0.76 -0.2 25,876 - GFF6530 0.81 -1.9 26,363 + -1.8 26,363 + -1.0 26,363 + -0.8 26,363 + -0.7 26,363 + +1.6 28,065 - GFF6532 0.27 -0.4 28,065 - GFF6532 0.27 +0.5 28,185 - GFF6532 0.36 -0.4 28,185 - GFF6532 0.36 -0.1 28,185 - GFF6532 0.36 +1.4 28,185 - GFF6532 0.36 -0.2 28,185 - GFF6532 0.36 +1.4 28,185 - GFF6532 0.36 -0.1 28,185 - GFF6532 0.36 -1.9 28,185 - GFF6532 0.36 -0.5 28,185 - GFF6532 0.36 +1.4 28,620 - GFF6532 0.67 -0.4 28,620 - GFF6532 0.67 -1.6 28,620 - GFF6532 0.67 +0.1 28,620 - GFF6532 0.67 -0.3
Or see this region's nucleotide sequence