Strain Fitness in Variovorax sp. SCN45 around GFF6429

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6427 and GFF6428 are separated by 48 nucleotidesGFF6428 and GFF6429 overlap by 4 nucleotidesGFF6429 and GFF6430 overlap by 4 nucleotides GFF6427 - ABC transporter, substrate-binding protein (cluster 7, di-/tri-carboxylate), at 974 to 1,939 GFF6427 GFF6428 - Glyoxalase/bleomycin resistance protein/dioxygenase, at 1,988 to 2,368 GFF6428 GFF6429 - Pyruvate:Oxaloacetate transcarboxylase domain protein, at 2,365 to 3,348 GFF6429 GFF6430 - L-carnitine dehydratase/bile acid-inducible protein F, at 3,345 to 4,517 GFF6430 Position (kb) 2 3 4Strain fitness (log2 ratio) -2 -1 0 1 2at 1.395 kb on + strand, within GFF6427at 1.395 kb on + strand, within GFF6427at 1.395 kb on + strand, within GFF6427at 1.396 kb on - strand, within GFF6427at 1.482 kb on + strand, within GFF6427at 1.482 kb on + strand, within GFF6427at 1.482 kb on + strand, within GFF6427at 1.483 kb on - strand, within GFF6427at 1.483 kb on - strand, within GFF6427at 1.483 kb on - strand, within GFF6427at 1.483 kb on - strand, within GFF6427at 1.659 kb on + strand, within GFF6427at 1.668 kb on + strand, within GFF6427at 1.669 kb on - strand, within GFF6427at 1.686 kb on + strand, within GFF6427at 1.687 kb on - strand, within GFF6427at 2.041 kb on - strand, within GFF6428at 2.041 kb on - strand, within GFF6428at 2.304 kb on + strand, within GFF6428at 2.304 kb on + strand, within GFF6428at 2.304 kb on + strand, within GFF6428at 2.304 kb on + strand, within GFF6428at 2.342 kb on + strandat 2.342 kb on + strandat 2.343 kb on - strandat 2.372 kb on + strandat 2.372 kb on + strandat 2.793 kb on - strand, within GFF6429at 3.113 kb on + strand, within GFF6429at 3.218 kb on + strand, within GFF6429at 3.536 kb on - strand, within GFF6430at 3.536 kb on - strand, within GFF6430at 3.568 kb on + strand, within GFF6430at 3.568 kb on + strand, within GFF6430at 3.568 kb on + strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.569 kb on - strand, within GFF6430at 3.570 kb on + strand, within GFF6430at 3.571 kb on - strand, within GFF6430at 3.787 kb on + strand, within GFF6430at 3.787 kb on + strand, within GFF6430at 3.787 kb on + strand, within GFF6430at 3.787 kb on + strand, within GFF6430at 3.787 kb on + strand, within GFF6430at 3.787 kb on + strand, within GFF6430at 3.788 kb on - strand, within GFF6430at 3.788 kb on - strand, within GFF6430at 3.788 kb on - strand, within GFF6430at 3.788 kb on - strand, within GFF6430at 3.788 kb on - strand, within GFF6430at 3.788 kb on - strand, within GFF6430at 3.809 kb on - strand, within GFF6430at 3.919 kb on + strand, within GFF6430at 4.072 kb on + strand, within GFF6430at 4.073 kb on - strand, within GFF6430

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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1,395 + GFF6427 0.44 +0.9
1,395 + GFF6427 0.44 -0.1
1,395 + GFF6427 0.44 -1.0
1,396 - GFF6427 0.44 +0.3
1,482 + GFF6427 0.53 -0.5
1,482 + GFF6427 0.53 +0.8
1,482 + GFF6427 0.53 +0.7
1,483 - GFF6427 0.53 +0.2
1,483 - GFF6427 0.53 -0.3
1,483 - GFF6427 0.53 -1.5
1,483 - GFF6427 0.53 +0.2
1,659 + GFF6427 0.71 -0.2
1,668 + GFF6427 0.72 +0.9
1,669 - GFF6427 0.72 +1.2
1,686 + GFF6427 0.74 -0.7
1,687 - GFF6427 0.74 +0.2
2,041 - GFF6428 0.14 -0.8
2,041 - GFF6428 0.14 +0.4
2,304 + GFF6428 0.83 -1.6
2,304 + GFF6428 0.83 -0.3
2,304 + GFF6428 0.83 -1.1
2,304 + GFF6428 0.83 +0.1
2,342 + +1.9
2,342 + +0.8
2,343 - -0.6
2,372 + +1.1
2,372 + -0.2
2,793 - GFF6429 0.43 -2.0
3,113 + GFF6429 0.76 -0.0
3,218 + GFF6429 0.87 +0.6
3,536 - GFF6430 0.16 +0.2
3,536 - GFF6430 0.16 -0.5
3,568 + GFF6430 0.19 -0.2
3,568 + GFF6430 0.19 -0.1
3,568 + GFF6430 0.19 +0.2
3,569 - GFF6430 0.19 -0.4
3,569 - GFF6430 0.19 +0.2
3,569 - GFF6430 0.19 +1.0
3,569 - GFF6430 0.19 -2.6
3,569 - GFF6430 0.19 -1.3
3,569 - GFF6430 0.19 -0.4
3,569 - GFF6430 0.19 -1.5
3,569 - GFF6430 0.19 -0.6
3,569 - GFF6430 0.19 -0.2
3,570 + GFF6430 0.19 -0.0
3,571 - GFF6430 0.19 +0.1
3,787 + GFF6430 0.38 +0.1
3,787 + GFF6430 0.38 -0.6
3,787 + GFF6430 0.38 +0.4
3,787 + GFF6430 0.38 -1.0
3,787 + GFF6430 0.38 -0.0
3,787 + GFF6430 0.38 -0.3
3,788 - GFF6430 0.38 -0.4
3,788 - GFF6430 0.38 +0.3
3,788 - GFF6430 0.38 +1.4
3,788 - GFF6430 0.38 -0.5
3,788 - GFF6430 0.38 -0.2
3,788 - GFF6430 0.38 +1.0
3,809 - GFF6430 0.40 +0.7
3,919 + GFF6430 0.49 +0.9
4,072 + GFF6430 0.62 +0.2
4,073 - GFF6430 0.62 -0.2

Or see this region's nucleotide sequence