Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6273 and GFF6274 are separated by 65 nucleotides GFF6274 and GFF6275 overlap by 4 nucleotides GFF6275 and GFF6276 overlap by 11 nucleotides GFF6276 and GFF6277 are separated by 46 nucleotides GFF6277 and GFF6278 are separated by 22 nucleotides
GFF6273 - Nicotinamide-nucleotide amidase (EC 3.5.1.42), at 64,409 to 64,918
GFF6273
GFF6274 - Ketosteroid isomerase-related protein, at 64,984 to 65,478
GFF6274
GFF6275 - hypothetical protein, at 65,475 to 65,795
GFF6275
GFF6276 - Uncharacterized MFS-type transporter, at 65,785 to 66,972
GFF6276
GFF6277 - YecA-like protein, at 67,019 to 67,804
GFF6277
GFF6278 - Ferredoxin-dependent glutamate synthase (EC 1.4.7.1), at 67,827 to 69,557
GFF6278
Position (kb) 65
66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 64.877 kb on + strand at 64.877 kb on + strand at 64.878 kb on - strand at 64.878 kb on - strand at 64.944 kb on + strand at 64.945 kb on - strand at 64.945 kb on - strand at 64.945 kb on - strand at 64.945 kb on - strand at 65.023 kb on + strand at 65.023 kb on + strand at 65.023 kb on + strand at 65.024 kb on - strand at 65.024 kb on - strand at 65.024 kb on - strand at 65.024 kb on - strand at 65.024 kb on - strand at 65.024 kb on - strand at 65.024 kb on - strand at 65.068 kb on + strand, within GFF6274 at 65.068 kb on + strand, within GFF6274 at 65.068 kb on + strand, within GFF6274 at 65.069 kb on - strand, within GFF6274 at 65.069 kb on - strand, within GFF6274 at 65.069 kb on - strand, within GFF6274 at 65.069 kb on - strand, within GFF6274 at 65.248 kb on + strand, within GFF6274 at 65.248 kb on + strand, within GFF6274 at 65.458 kb on + strand at 65.458 kb on + strand at 65.458 kb on + strand at 65.458 kb on + strand at 65.458 kb on + strand at 65.459 kb on - strand at 65.459 kb on - strand at 65.459 kb on - strand at 65.459 kb on - strand at 65.459 kb on - strand at 65.459 kb on - strand at 65.459 kb on - strand at 65.459 kb on - strand at 65.459 kb on - strand at 65.529 kb on + strand, within GFF6275 at 65.530 kb on - strand, within GFF6275 at 65.562 kb on + strand, within GFF6275 at 65.562 kb on + strand, within GFF6275 at 65.562 kb on + strand, within GFF6275 at 65.563 kb on - strand, within GFF6275 at 65.563 kb on - strand, within GFF6275 at 65.563 kb on - strand, within GFF6275 at 65.563 kb on - strand, within GFF6275 at 65.563 kb on - strand, within GFF6275 at 65.571 kb on + strand, within GFF6275 at 65.571 kb on + strand, within GFF6275 at 65.571 kb on + strand, within GFF6275 at 65.571 kb on + strand, within GFF6275 at 65.571 kb on + strand, within GFF6275 at 65.571 kb on + strand, within GFF6275 at 65.572 kb on - strand, within GFF6275 at 65.572 kb on - strand, within GFF6275 at 65.572 kb on - strand, within GFF6275 at 65.572 kb on - strand, within GFF6275 at 65.572 kb on - strand, within GFF6275 at 65.572 kb on - strand, within GFF6275 at 65.572 kb on - strand, within GFF6275 at 65.727 kb on + strand, within GFF6275 at 65.727 kb on + strand, within GFF6275 at 65.727 kb on + strand, within GFF6275 at 65.727 kb on + strand, within GFF6275 at 65.728 kb on - strand, within GFF6275 at 65.728 kb on - strand, within GFF6275 at 65.728 kb on - strand, within GFF6275 at 65.728 kb on - strand, within GFF6275 at 65.728 kb on - strand, within GFF6275 at 65.728 kb on - strand, within GFF6275 at 65.728 kb on - strand, within GFF6275 at 65.728 kb on - strand, within GFF6275 at 65.748 kb on + strand, within GFF6275 at 65.749 kb on - strand, within GFF6275 at 65.749 kb on - strand, within GFF6275 at 65.749 kb on - strand, within GFF6275 at 66.008 kb on + strand, within GFF6276 at 66.009 kb on - strand, within GFF6276 at 66.029 kb on + strand, within GFF6276 at 66.029 kb on + strand, within GFF6276 at 66.030 kb on - strand, within GFF6276 at 66.030 kb on - strand, within GFF6276 at 66.131 kb on + strand, within GFF6276 at 66.131 kb on + strand, within GFF6276 at 66.132 kb on - strand, within GFF6276 at 66.132 kb on - strand, within GFF6276 at 66.132 kb on - strand, within GFF6276 at 66.263 kb on + strand, within GFF6276 at 66.263 kb on + strand, within GFF6276 at 66.264 kb on - strand, within GFF6276 at 66.264 kb on - strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.326 kb on + strand, within GFF6276 at 66.327 kb on - strand, within GFF6276 at 66.327 kb on - strand, within GFF6276 at 66.327 kb on - strand, within GFF6276 at 66.327 kb on - strand, within GFF6276 at 66.330 kb on + strand, within GFF6276 at 66.330 kb on + strand, within GFF6276 at 66.330 kb on + strand, within GFF6276 at 66.331 kb on - strand, within GFF6276 at 66.455 kb on + strand, within GFF6276 at 66.455 kb on + strand, within GFF6276 at 66.456 kb on - strand, within GFF6276 at 66.456 kb on - strand, within GFF6276 at 66.521 kb on + strand, within GFF6276 at 66.521 kb on + strand, within GFF6276 at 66.521 kb on + strand, within GFF6276 at 66.521 kb on + strand, within GFF6276 at 66.521 kb on + strand, within GFF6276 at 66.521 kb on + strand, within GFF6276 at 66.521 kb on + strand, within GFF6276 at 66.521 kb on + strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.522 kb on - strand, within GFF6276 at 66.710 kb on + strand, within GFF6276 at 66.710 kb on + strand, within GFF6276 at 66.710 kb on + strand, within GFF6276 at 66.710 kb on + strand, within GFF6276 at 66.710 kb on + strand, within GFF6276 at 66.710 kb on + strand, within GFF6276 at 66.710 kb on + strand, within GFF6276 at 66.710 kb on + strand, within GFF6276 at 66.711 kb on - strand, within GFF6276 at 66.711 kb on - strand, within GFF6276 at 66.711 kb on - strand, within GFF6276 at 66.711 kb on - strand, within GFF6276 at 66.711 kb on - strand, within GFF6276 at 66.711 kb on - strand, within GFF6276 at 67.044 kb on + strand at 67.045 kb on - strand at 67.152 kb on + strand, within GFF6277 at 67.152 kb on + strand, within GFF6277 at 67.153 kb on - strand, within GFF6277 at 67.153 kb on - strand, within GFF6277 at 67.153 kb on - strand, within GFF6277 at 67.153 kb on - strand, within GFF6277 at 67.153 kb on - strand, within GFF6277 at 67.153 kb on - strand, within GFF6277 at 67.224 kb on + strand, within GFF6277 at 67.224 kb on + strand, within GFF6277 at 67.224 kb on + strand, within GFF6277 at 67.224 kb on + strand, within GFF6277 at 67.224 kb on + strand, within GFF6277 at 67.224 kb on + strand, within GFF6277 at 67.224 kb on + strand, within GFF6277 at 67.225 kb on - strand, within GFF6277 at 67.225 kb on - strand, within GFF6277 at 67.225 kb on - strand, within GFF6277 at 67.225 kb on - strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.356 kb on + strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.357 kb on - strand, within GFF6277 at 67.611 kb on + strand, within GFF6277 at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.826 kb on + strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.853 kb on + strand at 67.853 kb on + strand at 67.853 kb on + strand at 67.853 kb on + strand at 67.853 kb on + strand at 67.853 kb on + strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.854 kb on - strand at 67.859 kb on + strand at 67.859 kb on + strand at 67.859 kb on + strand at 67.859 kb on + strand at 67.859 kb on + strand at 67.859 kb on + strand at 67.860 kb on - strand at 67.860 kb on - strand at 67.860 kb on - strand at 67.871 kb on + strand at 67.871 kb on + strand at 67.871 kb on + strand at 67.871 kb on + strand at 67.871 kb on + strand at 67.871 kb on + strand at 67.871 kb on + strand at 67.872 kb on - strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.890 kb on + strand at 67.891 kb on - strand at 67.891 kb on - strand at 67.891 kb on - strand at 67.891 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 64,877 + +1.1 64,877 + +0.1 64,878 - -0.3 64,878 - -0.3 64,944 + -0.1 64,945 - -2.0 64,945 - -0.2 64,945 - +2.3 64,945 - +0.2 65,023 + -0.1 65,023 + -1.1 65,023 + -0.1 65,024 - +0.5 65,024 - -0.9 65,024 - +0.1 65,024 - +1.3 65,024 - -0.5 65,024 - +0.4 65,024 - -0.5 65,068 + GFF6274 0.17 +0.4 65,068 + GFF6274 0.17 +0.6 65,068 + GFF6274 0.17 +1.7 65,069 - GFF6274 0.17 -0.0 65,069 - GFF6274 0.17 +0.1 65,069 - GFF6274 0.17 -0.1 65,069 - GFF6274 0.17 -0.4 65,248 + GFF6274 0.53 -0.0 65,248 + GFF6274 0.53 +0.1 65,458 + -0.6 65,458 + +1.4 65,458 + -0.0 65,458 + -0.3 65,458 + -1.0 65,459 - -0.5 65,459 - +1.4 65,459 - -0.1 65,459 - -0.7 65,459 - -0.6 65,459 - +0.3 65,459 - -0.7 65,459 - +1.0 65,459 - -1.1 65,529 + GFF6275 0.17 -1.5 65,530 - GFF6275 0.17 +0.0 65,562 + GFF6275 0.27 -0.4 65,562 + GFF6275 0.27 +0.1 65,562 + GFF6275 0.27 +0.6 65,563 - GFF6275 0.27 -2.1 65,563 - GFF6275 0.27 -0.7 65,563 - GFF6275 0.27 +0.1 65,563 - GFF6275 0.27 +0.4 65,563 - GFF6275 0.27 +0.4 65,571 + GFF6275 0.30 -0.7 65,571 + GFF6275 0.30 -0.6 65,571 + GFF6275 0.30 -0.5 65,571 + GFF6275 0.30 +0.7 65,571 + GFF6275 0.30 -0.3 65,571 + GFF6275 0.30 +0.9 65,572 - GFF6275 0.30 +0.7 65,572 - GFF6275 0.30 -0.7 65,572 - GFF6275 0.30 -0.7 65,572 - GFF6275 0.30 -0.9 65,572 - GFF6275 0.30 +0.7 65,572 - GFF6275 0.30 -0.5 65,572 - GFF6275 0.30 -0.2 65,727 + GFF6275 0.79 +0.0 65,727 + GFF6275 0.79 +2.3 65,727 + GFF6275 0.79 -0.0 65,727 + GFF6275 0.79 +1.1 65,728 - GFF6275 0.79 -0.6 65,728 - GFF6275 0.79 -0.2 65,728 - GFF6275 0.79 -0.0 65,728 - GFF6275 0.79 -1.2 65,728 - GFF6275 0.79 -0.1 65,728 - GFF6275 0.79 +0.2 65,728 - GFF6275 0.79 +0.4 65,728 - GFF6275 0.79 -0.5 65,748 + GFF6275 0.85 -1.4 65,749 - GFF6275 0.85 -0.7 65,749 - GFF6275 0.85 +0.3 65,749 - GFF6275 0.85 -0.5 66,008 + GFF6276 0.19 -0.2 66,009 - GFF6276 0.19 +0.2 66,029 + GFF6276 0.21 -0.2 66,029 + GFF6276 0.21 -1.9 66,030 - GFF6276 0.21 -0.4 66,030 - GFF6276 0.21 +0.1 66,131 + GFF6276 0.29 +0.9 66,131 + GFF6276 0.29 -0.1 66,132 - GFF6276 0.29 +0.4 66,132 - GFF6276 0.29 -2.6 66,132 - GFF6276 0.29 -0.1 66,263 + GFF6276 0.40 -0.6 66,263 + GFF6276 0.40 +0.1 66,264 - GFF6276 0.40 -0.7 66,264 - GFF6276 0.40 -0.9 66,326 + GFF6276 0.46 +0.1 66,326 + GFF6276 0.46 -0.1 66,326 + GFF6276 0.46 +0.3 66,326 + GFF6276 0.46 -0.5 66,326 + GFF6276 0.46 -0.3 66,326 + GFF6276 0.46 +0.3 66,326 + GFF6276 0.46 -1.0 66,326 + GFF6276 0.46 +1.9 66,326 + GFF6276 0.46 -0.8 66,326 + GFF6276 0.46 -0.8 66,326 + GFF6276 0.46 +0.0 66,327 - GFF6276 0.46 +0.1 66,327 - GFF6276 0.46 +1.1 66,327 - GFF6276 0.46 -0.2 66,327 - GFF6276 0.46 -0.9 66,330 + GFF6276 0.46 +0.5 66,330 + GFF6276 0.46 -0.5 66,330 + GFF6276 0.46 +0.9 66,331 - GFF6276 0.46 +0.7 66,455 + GFF6276 0.56 -0.1 66,455 + GFF6276 0.56 -1.0 66,456 - GFF6276 0.56 -0.2 66,456 - GFF6276 0.56 +0.5 66,521 + GFF6276 0.62 +0.7 66,521 + GFF6276 0.62 +0.3 66,521 + GFF6276 0.62 -0.4 66,521 + GFF6276 0.62 +0.1 66,521 + GFF6276 0.62 -0.2 66,521 + GFF6276 0.62 -1.3 66,521 + GFF6276 0.62 -0.3 66,521 + GFF6276 0.62 +1.1 66,522 - GFF6276 0.62 +2.3 66,522 - GFF6276 0.62 +0.5 66,522 - GFF6276 0.62 -1.1 66,522 - GFF6276 0.62 -0.1 66,522 - GFF6276 0.62 -1.5 66,522 - GFF6276 0.62 +1.7 66,522 - GFF6276 0.62 -0.1 66,522 - GFF6276 0.62 -0.0 66,522 - GFF6276 0.62 +0.6 66,522 - GFF6276 0.62 -0.3 66,522 - GFF6276 0.62 +3.3 66,522 - GFF6276 0.62 -2.8 66,522 - GFF6276 0.62 -1.7 66,522 - GFF6276 0.62 -1.0 66,522 - GFF6276 0.62 +0.4 66,710 + GFF6276 0.78 +0.4 66,710 + GFF6276 0.78 +0.5 66,710 + GFF6276 0.78 -0.2 66,710 + GFF6276 0.78 +0.3 66,710 + GFF6276 0.78 +1.7 66,710 + GFF6276 0.78 -0.3 66,710 + GFF6276 0.78 -1.3 66,710 + GFF6276 0.78 +0.0 66,711 - GFF6276 0.78 +0.8 66,711 - GFF6276 0.78 -1.3 66,711 - GFF6276 0.78 +0.1 66,711 - GFF6276 0.78 +0.1 66,711 - GFF6276 0.78 -0.0 66,711 - GFF6276 0.78 +0.7 67,044 + +1.1 67,045 - -1.3 67,152 + GFF6277 0.17 -0.0 67,152 + GFF6277 0.17 -1.5 67,153 - GFF6277 0.17 +1.7 67,153 - GFF6277 0.17 -2.2 67,153 - GFF6277 0.17 -0.3 67,153 - GFF6277 0.17 -0.9 67,153 - GFF6277 0.17 +0.1 67,153 - GFF6277 0.17 -0.0 67,224 + GFF6277 0.26 +0.1 67,224 + GFF6277 0.26 -0.2 67,224 + GFF6277 0.26 -0.5 67,224 + GFF6277 0.26 +0.4 67,224 + GFF6277 0.26 -0.3 67,224 + GFF6277 0.26 -0.6 67,224 + GFF6277 0.26 -0.1 67,225 - GFF6277 0.26 -1.2 67,225 - GFF6277 0.26 -0.2 67,225 - GFF6277 0.26 -0.2 67,225 - GFF6277 0.26 +0.2 67,356 + GFF6277 0.43 +0.4 67,356 + GFF6277 0.43 -1.8 67,356 + GFF6277 0.43 -0.3 67,356 + GFF6277 0.43 -0.2 67,356 + GFF6277 0.43 -0.3 67,356 + GFF6277 0.43 +0.6 67,356 + GFF6277 0.43 -0.1 67,356 + GFF6277 0.43 -0.8 67,356 + GFF6277 0.43 -0.2 67,357 - GFF6277 0.43 -0.9 67,357 - GFF6277 0.43 -1.3 67,357 - GFF6277 0.43 -0.5 67,357 - GFF6277 0.43 -0.6 67,357 - GFF6277 0.43 +0.3 67,357 - GFF6277 0.43 -0.3 67,357 - GFF6277 0.43 -0.5 67,357 - GFF6277 0.43 -1.7 67,357 - GFF6277 0.43 -0.2 67,357 - GFF6277 0.43 +0.1 67,357 - GFF6277 0.43 +2.0 67,357 - GFF6277 0.43 -0.0 67,611 + GFF6277 0.75 -1.3 67,826 + -0.8 67,826 + +0.0 67,826 + -0.9 67,826 + -0.3 67,826 + +1.5 67,826 + +1.1 67,826 + +0.2 67,826 + -0.4 67,826 + +0.9 67,826 + -0.8 67,826 + -0.2 67,826 + -1.5 67,826 + -0.3 67,826 + -1.1 67,826 + -1.1 67,827 - +0.5 67,827 - -0.3 67,827 - -1.3 67,827 - -0.3 67,827 - -0.2 67,827 - +0.4 67,827 - +0.0 67,827 - -0.1 67,827 - -0.0 67,827 - -0.9 67,827 - +0.0 67,827 - +0.6 67,827 - -0.7 67,827 - +0.7 67,827 - +0.7 67,827 - +0.6 67,853 + -0.5 67,853 + -0.3 67,853 + -0.8 67,853 + +0.7 67,853 + -1.3 67,853 + +0.3 67,854 - -0.3 67,854 - -0.9 67,854 - -0.5 67,854 - +1.3 67,854 - -1.2 67,859 + -0.4 67,859 + +0.1 67,859 + -0.5 67,859 + +1.1 67,859 + +0.1 67,859 + -0.6 67,860 - -0.7 67,860 - -0.1 67,860 - -0.3 67,871 + +0.9 67,871 + +0.6 67,871 + -0.4 67,871 + -1.7 67,871 + +0.3 67,871 + +0.0 67,871 + +0.2 67,872 - -1.6 67,890 + -0.2 67,890 + -1.0 67,890 + -0.5 67,890 + +0.3 67,890 + -0.7 67,890 + -0.4 67,890 + -0.2 67,890 + +0.3 67,890 + -1.4 67,890 + +0.1 67,890 + -0.2 67,891 - -2.1 67,891 - +0.1 67,891 - -1.2 67,891 - -0.9
Or see this region's nucleotide sequence