Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6255 and GFF6256 overlap by 4 nucleotides GFF6256 and GFF6257 overlap by 4 nucleotides GFF6257 and GFF6258 overlap by 4 nucleotides GFF6258 and GFF6259 overlap by 4 nucleotides
GFF6255 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (EC 3.6.1.63), at 48,834 to 49,988
GFF6255
GFF6256 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnL (EC 2.7.8.37), at 49,985 to 50,698
GFF6256
GFF6257 - Phosphonates utilization ATP-binding protein PhnK, at 50,695 to 51,483
GFF6257
GFF6258 - Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase (EC 4.7.1.1), at 51,480 to 52,352
GFF6258
GFF6259 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI (EC 2.7.8.37), at 52,349 to 53,446
GFF6259
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 50.451 kb on - strand, within GFF6256 at 50.451 kb on - strand, within GFF6256 at 50.514 kb on + strand, within GFF6256 at 50.515 kb on - strand, within GFF6256 at 50.523 kb on + strand, within GFF6256 at 50.523 kb on + strand, within GFF6256 at 50.523 kb on + strand, within GFF6256 at 50.625 kb on - strand, within GFF6256 at 50.732 kb on + strand at 50.732 kb on + strand at 50.732 kb on + strand at 50.733 kb on - strand at 51.426 kb on - strand at 51.697 kb on + strand, within GFF6258 at 51.698 kb on - strand, within GFF6258 at 51.713 kb on - strand, within GFF6258 at 51.713 kb on - strand, within GFF6258 at 51.713 kb on - strand, within GFF6258 at 51.713 kb on - strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.841 kb on + strand, within GFF6258 at 51.842 kb on - strand, within GFF6258 at 51.842 kb on - strand, within GFF6258 at 51.880 kb on + strand, within GFF6258 at 51.986 kb on - strand, within GFF6258 at 52.322 kb on - strand at 52.322 kb on - strand at 52.333 kb on + strand at 52.333 kb on + strand at 52.333 kb on + strand at 52.334 kb on - strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand at 52.419 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 50,451 - GFF6256 0.65 -0.1 50,451 - GFF6256 0.65 -0.1 50,514 + GFF6256 0.74 +0.5 50,515 - GFF6256 0.74 +1.1 50,523 + GFF6256 0.75 -1.5 50,523 + GFF6256 0.75 -0.5 50,523 + GFF6256 0.75 +0.4 50,625 - GFF6256 0.90 -0.0 50,732 + +0.7 50,732 + +0.3 50,732 + -0.4 50,733 - -0.6 51,426 - -0.2 51,697 + GFF6258 0.25 -0.3 51,698 - GFF6258 0.25 +0.3 51,713 - GFF6258 0.27 +0.4 51,713 - GFF6258 0.27 -0.5 51,713 - GFF6258 0.27 -0.1 51,713 - GFF6258 0.27 -1.0 51,841 + GFF6258 0.41 -0.6 51,841 + GFF6258 0.41 -0.2 51,841 + GFF6258 0.41 -1.6 51,841 + GFF6258 0.41 -1.1 51,841 + GFF6258 0.41 +0.0 51,841 + GFF6258 0.41 -0.9 51,842 - GFF6258 0.41 -0.1 51,842 - GFF6258 0.41 -1.0 51,880 + GFF6258 0.46 -0.3 51,986 - GFF6258 0.58 +3.0 52,322 - -0.2 52,322 - +2.1 52,333 + +0.4 52,333 + -0.3 52,333 + +0.8 52,334 - +0.5 52,419 + -1.9 52,419 + +0.3 52,419 + +0.3 52,419 + -0.1 52,419 + +0.7 52,419 + -0.2 52,419 + -0.3 52,419 + -1.1
Or see this region's nucleotide sequence