Strain Fitness in Variovorax sp. SCN45 around GFF6257

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6255 and GFF6256 overlap by 4 nucleotidesGFF6256 and GFF6257 overlap by 4 nucleotidesGFF6257 and GFF6258 overlap by 4 nucleotidesGFF6258 and GFF6259 overlap by 4 nucleotides GFF6255 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (EC 3.6.1.63), at 48,834 to 49,988 GFF6255 GFF6256 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnL (EC 2.7.8.37), at 49,985 to 50,698 GFF6256 GFF6257 - Phosphonates utilization ATP-binding protein PhnK, at 50,695 to 51,483 GFF6257 GFF6258 - Alpha-D-ribose 1-methylphosphonate 5-phosphate C-P lyase (EC 4.7.1.1), at 51,480 to 52,352 GFF6258 GFF6259 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnI (EC 2.7.8.37), at 52,349 to 53,446 GFF6259 Position (kb) 50 51 52Strain fitness (log2 ratio) -2 -1 0 1 2 3at 50.451 kb on - strand, within GFF6256at 50.451 kb on - strand, within GFF6256at 50.514 kb on + strand, within GFF6256at 50.515 kb on - strand, within GFF6256at 50.523 kb on + strand, within GFF6256at 50.523 kb on + strand, within GFF6256at 50.523 kb on + strand, within GFF6256at 50.625 kb on - strand, within GFF6256at 50.732 kb on + strandat 50.732 kb on + strandat 50.732 kb on + strandat 50.733 kb on - strandat 51.426 kb on - strandat 51.697 kb on + strand, within GFF6258at 51.698 kb on - strand, within GFF6258at 51.713 kb on - strand, within GFF6258at 51.713 kb on - strand, within GFF6258at 51.713 kb on - strand, within GFF6258at 51.713 kb on - strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.841 kb on + strand, within GFF6258at 51.842 kb on - strand, within GFF6258at 51.842 kb on - strand, within GFF6258at 51.880 kb on + strand, within GFF6258at 51.986 kb on - strand, within GFF6258at 52.322 kb on - strandat 52.322 kb on - strandat 52.333 kb on + strandat 52.333 kb on + strandat 52.333 kb on + strandat 52.334 kb on - strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strandat 52.419 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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50,451 - GFF6256 0.65 -0.1
50,451 - GFF6256 0.65 -0.1
50,514 + GFF6256 0.74 +0.5
50,515 - GFF6256 0.74 +1.1
50,523 + GFF6256 0.75 -1.5
50,523 + GFF6256 0.75 -0.5
50,523 + GFF6256 0.75 +0.4
50,625 - GFF6256 0.90 -0.0
50,732 + +0.7
50,732 + +0.3
50,732 + -0.4
50,733 - -0.6
51,426 - -0.2
51,697 + GFF6258 0.25 -0.3
51,698 - GFF6258 0.25 +0.3
51,713 - GFF6258 0.27 +0.4
51,713 - GFF6258 0.27 -0.5
51,713 - GFF6258 0.27 -0.1
51,713 - GFF6258 0.27 -1.0
51,841 + GFF6258 0.41 -0.6
51,841 + GFF6258 0.41 -0.2
51,841 + GFF6258 0.41 -1.6
51,841 + GFF6258 0.41 -1.1
51,841 + GFF6258 0.41 +0.0
51,841 + GFF6258 0.41 -0.9
51,842 - GFF6258 0.41 -0.1
51,842 - GFF6258 0.41 -1.0
51,880 + GFF6258 0.46 -0.3
51,986 - GFF6258 0.58 +3.0
52,322 - -0.2
52,322 - +2.1
52,333 + +0.4
52,333 + -0.3
52,333 + +0.8
52,334 - +0.5
52,419 + -1.9
52,419 + +0.3
52,419 + +0.3
52,419 + -0.1
52,419 + +0.7
52,419 + -0.2
52,419 + -0.3
52,419 + -1.1

Or see this region's nucleotide sequence