Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6230 and GFF6231 are separated by 100 nucleotides GFF6231 and GFF6232 are separated by 9 nucleotides GFF6232 and GFF6233 are separated by 2 nucleotides
GFF6230 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 14,563 to 15,540
GFF6230
GFF6231 - Amidases related to nicotinamidase, at 15,641 to 16,273
GFF6231
GFF6232 - hypothetical protein, at 16,283 to 21,721
GFF6232
GFF6233 - hypothetical protein, at 21,724 to 23,433
GFF6233
Position (kb)
16
17
18
19
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 15.810 kb on + strand, within GFF6231 at 15.811 kb on - strand, within GFF6231 at 15.998 kb on + strand, within GFF6231 at 15.999 kb on - strand, within GFF6231 at 15.999 kb on - strand, within GFF6231 at 16.031 kb on + strand, within GFF6231 at 16.032 kb on - strand, within GFF6231 at 16.032 kb on - strand, within GFF6231 at 16.032 kb on - strand, within GFF6231 at 16.095 kb on - strand, within GFF6231 at 16.095 kb on - strand, within GFF6231 at 16.455 kb on + strand at 16.455 kb on + strand at 16.456 kb on - strand at 16.456 kb on - strand at 16.551 kb on + strand at 16.552 kb on - strand at 16.552 kb on - strand at 16.758 kb on + strand at 16.759 kb on - strand at 16.759 kb on - strand at 16.821 kb on + strand at 16.821 kb on + strand at 16.833 kb on + strand, within GFF6232 at 16.833 kb on + strand, within GFF6232 at 16.833 kb on + strand, within GFF6232 at 16.834 kb on - strand, within GFF6232 at 16.917 kb on + strand, within GFF6232 at 16.918 kb on - strand, within GFF6232 at 16.918 kb on - strand, within GFF6232 at 16.918 kb on - strand, within GFF6232 at 17.130 kb on + strand, within GFF6232 at 17.130 kb on + strand, within GFF6232 at 17.131 kb on - strand, within GFF6232 at 17.131 kb on - strand, within GFF6232 at 17.895 kb on + strand, within GFF6232 at 17.895 kb on + strand, within GFF6232 at 17.896 kb on - strand, within GFF6232 at 17.896 kb on - strand, within GFF6232 at 17.901 kb on + strand, within GFF6232 at 17.901 kb on + strand, within GFF6232 at 17.901 kb on + strand, within GFF6232 at 17.901 kb on + strand, within GFF6232 at 17.902 kb on - strand, within GFF6232 at 17.902 kb on - strand, within GFF6232 at 17.902 kb on - strand, within GFF6232 at 18.262 kb on - strand, within GFF6232 at 18.262 kb on - strand, within GFF6232 at 18.262 kb on - strand, within GFF6232 at 18.262 kb on - strand, within GFF6232 at 18.262 kb on - strand, within GFF6232 at 18.924 kb on + strand, within GFF6232 at 19.128 kb on + strand, within GFF6232 at 19.497 kb on + strand, within GFF6232 at 19.498 kb on - strand, within GFF6232 at 19.707 kb on + strand, within GFF6232 at 19.707 kb on + strand, within GFF6232 at 19.708 kb on - strand, within GFF6232 at 19.708 kb on - strand, within GFF6232 at 19.710 kb on + strand, within GFF6232 at 19.711 kb on - strand, within GFF6232 at 19.711 kb on - strand, within GFF6232 at 19.914 kb on + strand, within GFF6232 at 19.915 kb on - strand, within GFF6232 at 19.915 kb on - strand, within GFF6232 at 20.266 kb on - strand, within GFF6232 at 20.629 kb on - strand, within GFF6232 at 20.629 kb on - strand, within GFF6232 at 21.750 kb on - strand at 21.899 kb on + strand, within GFF6233 at 21.900 kb on - strand, within GFF6233 at 22.121 kb on + strand, within GFF6233 at 22.121 kb on + strand, within GFF6233 at 22.122 kb on - strand, within GFF6233 at 22.122 kb on - strand, within GFF6233 at 22.454 kb on + strand, within GFF6233 at 22.526 kb on + strand, within GFF6233 at 22.526 kb on + strand, within GFF6233 at 22.586 kb on + strand, within GFF6233 at 22.587 kb on - strand, within GFF6233 at 22.698 kb on - strand, within GFF6233 at 22.721 kb on + strand, within GFF6233 at 22.721 kb on + strand, within GFF6233
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 15,810 + GFF6231 0.27 -0.3 15,811 - GFF6231 0.27 -0.3 15,998 + GFF6231 0.56 +0.5 15,999 - GFF6231 0.57 +1.7 15,999 - GFF6231 0.57 +0.8 16,031 + GFF6231 0.62 -0.7 16,032 - GFF6231 0.62 -0.9 16,032 - GFF6231 0.62 +0.9 16,032 - GFF6231 0.62 +0.7 16,095 - GFF6231 0.72 -0.4 16,095 - GFF6231 0.72 -1.5 16,455 + -0.0 16,455 + +0.5 16,456 - +1.1 16,456 - -0.2 16,551 + -1.0 16,552 - -0.4 16,552 - -0.2 16,758 + -0.2 16,759 - -0.2 16,759 - -0.8 16,821 + -0.4 16,821 + +0.1 16,833 + GFF6232 0.10 -0.8 16,833 + GFF6232 0.10 -0.8 16,833 + GFF6232 0.10 -0.9 16,834 - GFF6232 0.10 +0.4 16,917 + GFF6232 0.12 -0.0 16,918 - GFF6232 0.12 -0.3 16,918 - GFF6232 0.12 -0.0 16,918 - GFF6232 0.12 +0.7 17,130 + GFF6232 0.16 -0.2 17,130 + GFF6232 0.16 +0.0 17,131 - GFF6232 0.16 -0.2 17,131 - GFF6232 0.16 -1.6 17,895 + GFF6232 0.30 -0.0 17,895 + GFF6232 0.30 -0.3 17,896 - GFF6232 0.30 -1.1 17,896 - GFF6232 0.30 -0.9 17,901 + GFF6232 0.30 -0.5 17,901 + GFF6232 0.30 -2.2 17,901 + GFF6232 0.30 -1.2 17,901 + GFF6232 0.30 -1.1 17,902 - GFF6232 0.30 +1.9 17,902 - GFF6232 0.30 -0.7 17,902 - GFF6232 0.30 -0.2 18,262 - GFF6232 0.36 -1.2 18,262 - GFF6232 0.36 +0.5 18,262 - GFF6232 0.36 -0.2 18,262 - GFF6232 0.36 +0.2 18,262 - GFF6232 0.36 -0.1 18,924 + GFF6232 0.49 +0.7 19,128 + GFF6232 0.52 -0.6 19,497 + GFF6232 0.59 -0.0 19,498 - GFF6232 0.59 -0.1 19,707 + GFF6232 0.63 -1.1 19,707 + GFF6232 0.63 -0.1 19,708 - GFF6232 0.63 -0.1 19,708 - GFF6232 0.63 -0.6 19,710 + GFF6232 0.63 -1.1 19,711 - GFF6232 0.63 -1.1 19,711 - GFF6232 0.63 -1.6 19,914 + GFF6232 0.67 -1.3 19,915 - GFF6232 0.67 -0.6 19,915 - GFF6232 0.67 -0.1 20,266 - GFF6232 0.73 +0.5 20,629 - GFF6232 0.80 -0.6 20,629 - GFF6232 0.80 -0.2 21,750 - +1.9 21,899 + GFF6233 0.10 +0.2 21,900 - GFF6233 0.10 -0.9 22,121 + GFF6233 0.23 -0.5 22,121 + GFF6233 0.23 +0.2 22,122 - GFF6233 0.23 -0.3 22,122 - GFF6233 0.23 +0.5 22,454 + GFF6233 0.43 +1.3 22,526 + GFF6233 0.47 +1.7 22,526 + GFF6233 0.47 -0.5 22,586 + GFF6233 0.50 +0.3 22,587 - GFF6233 0.50 -0.8 22,698 - GFF6233 0.57 -1.1 22,721 + GFF6233 0.58 +0.6 22,721 + GFF6233 0.58 +1.5
Or see this region's nucleotide sequence