Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6173 and GFF6174 are separated by 76 nucleotides GFF6174 and GFF6175 are separated by 84 nucleotides GFF6175 and GFF6176 are separated by 138 nucleotides GFF6176 and GFF6177 overlap by 11 nucleotides GFF6177 and GFF6178 are separated by 57 nucleotides GFF6178 and GFF6179 are separated by 71 nucleotides
GFF6173 - Na(+) H(+) antiporter subunit A / Na(+) H(+) antiporter subunit B, at 76,731 to 79,679
GFF6173
GFF6174 - Fluoride ion transporter CrcB, at 79,756 to 80,133
GFF6174
GFF6175 - hypothetical protein, at 80,218 to 80,535
GFF6175
GFF6176 - Lipopolysaccharide assembly protein LapB, at 80,674 to 81,843
GFF6176
GFF6177 - Putative transmembrane protein, at 81,833 to 82,171
GFF6177
GFF6178 - Integration host factor beta subunit, at 82,229 to 82,555
GFF6178
GFF6179 - SSU ribosomal protein S1p, at 82,627 to 84,315
GFF6179
Position (kb) 80
81
82 Strain fitness (log2 ratio) -2
-1
0
1
2 at 79.705 kb on + strand at 79.705 kb on + strand at 79.706 kb on - strand at 79.717 kb on + strand at 79.717 kb on + strand at 79.718 kb on - strand at 79.718 kb on - strand at 79.721 kb on + strand at 79.722 kb on - strand at 79.722 kb on - strand at 79.736 kb on + strand at 79.737 kb on - strand at 79.737 kb on - strand at 79.737 kb on - strand at 79.737 kb on - strand at 79.737 kb on - strand at 80.001 kb on - strand, within GFF6174 at 80.001 kb on - strand, within GFF6174 at 80.378 kb on + strand, within GFF6175 at 80.535 kb on + strand at 80.535 kb on + strand at 80.536 kb on - strand at 80.536 kb on - strand at 80.536 kb on - strand at 80.536 kb on - strand at 80.536 kb on - strand at 80.540 kb on - strand at 80.656 kb on + strand at 80.656 kb on + strand at 80.656 kb on + strand at 80.656 kb on + strand at 80.656 kb on + strand at 80.656 kb on + strand at 80.657 kb on - strand at 80.657 kb on - strand at 80.657 kb on - strand at 80.657 kb on - strand at 81.851 kb on + strand at 81.851 kb on + strand at 81.852 kb on - strand at 81.852 kb on - strand at 81.852 kb on - strand at 81.852 kb on - strand at 81.852 kb on - strand at 82.065 kb on + strand, within GFF6177 at 82.065 kb on + strand, within GFF6177 at 82.066 kb on - strand, within GFF6177 at 82.066 kb on - strand, within GFF6177 at 82.066 kb on - strand, within GFF6177 at 82.066 kb on - strand, within GFF6177 at 82.066 kb on - strand, within GFF6177 at 82.083 kb on + strand, within GFF6177 at 82.083 kb on + strand, within GFF6177 at 82.083 kb on + strand, within GFF6177 at 82.083 kb on + strand, within GFF6177 at 82.083 kb on + strand, within GFF6177 at 82.084 kb on - strand, within GFF6177 at 82.084 kb on - strand, within GFF6177 at 82.084 kb on - strand, within GFF6177 at 82.084 kb on - strand, within GFF6177 at 82.164 kb on + strand at 82.164 kb on + strand at 82.164 kb on + strand at 82.164 kb on + strand at 82.165 kb on - strand at 82.165 kb on - strand at 82.198 kb on + strand at 82.198 kb on + strand at 82.198 kb on + strand at 82.555 kb on + strand at 82.555 kb on + strand at 82.556 kb on - strand at 82.556 kb on - strand at 82.628 kb on + strand at 82.628 kb on + strand at 82.629 kb on - strand at 82.629 kb on - strand at 82.629 kb on - strand at 82.629 kb on - strand at 82.629 kb on - strand at 82.629 kb on - strand at 82.629 kb on - strand at 82.629 kb on - strand at 82.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 79,705 + +0.0 79,705 + +0.5 79,706 - -0.2 79,717 + +0.4 79,717 + -0.5 79,718 - -0.8 79,718 - +0.3 79,721 + +0.0 79,722 - -0.8 79,722 - +0.7 79,736 + -0.8 79,737 - -0.3 79,737 - -0.3 79,737 - -0.5 79,737 - -0.3 79,737 - -1.3 80,001 - GFF6174 0.65 -0.1 80,001 - GFF6174 0.65 -0.6 80,378 + GFF6175 0.50 -0.2 80,535 + -0.2 80,535 + -0.4 80,536 - +0.5 80,536 - -0.4 80,536 - -0.2 80,536 - +1.5 80,536 - +0.5 80,540 - +0.6 80,656 + -1.4 80,656 + -0.4 80,656 + -0.1 80,656 + -0.0 80,656 + +0.3 80,656 + -0.5 80,657 - -0.1 80,657 - +0.3 80,657 - -0.2 80,657 - +1.3 81,851 + +0.2 81,851 + -0.3 81,852 - +0.4 81,852 - -0.3 81,852 - +0.4 81,852 - -0.3 81,852 - -1.1 82,065 + GFF6177 0.68 +0.1 82,065 + GFF6177 0.68 +1.5 82,066 - GFF6177 0.69 +2.2 82,066 - GFF6177 0.69 -0.7 82,066 - GFF6177 0.69 -0.7 82,066 - GFF6177 0.69 -0.7 82,066 - GFF6177 0.69 +0.9 82,083 + GFF6177 0.74 -0.5 82,083 + GFF6177 0.74 -0.4 82,083 + GFF6177 0.74 -0.4 82,083 + GFF6177 0.74 -0.3 82,083 + GFF6177 0.74 +0.4 82,084 - GFF6177 0.74 +0.4 82,084 - GFF6177 0.74 +0.2 82,084 - GFF6177 0.74 +0.8 82,084 - GFF6177 0.74 -0.4 82,164 + +0.1 82,164 + +0.6 82,164 + +0.4 82,164 + +1.5 82,165 - +0.3 82,165 - +1.1 82,198 + +0.2 82,198 + -0.5 82,198 + -0.7 82,555 + -0.3 82,555 + +2.1 82,556 - -2.0 82,556 - -0.5 82,628 + +1.5 82,628 + +0.7 82,629 - -0.2 82,629 - -0.3 82,629 - +0.7 82,629 - -1.2 82,629 - +0.9 82,629 - -0.8 82,629 - -0.7 82,629 - -1.1 82,629 - -0.5
Or see this region's nucleotide sequence