Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF613 and GFF614 overlap by 4 nucleotides GFF614 and GFF615 are separated by 76 nucleotides GFF615 and GFF616 are separated by 21 nucleotides GFF616 and GFF617 are separated by 175 nucleotides GFF617 and GFF618 overlap by 4 nucleotides
GFF613 - hypothetical protein, at 661,149 to 662,114
GFF613
GFF614 - no description, at 662,111 to 662,281
GFF614
GFF615 - beta-glucosidase (EC 3.2.1.21), at 662,358 to 664,643
GFF615
GFF616 - hypothetical protein, at 664,665 to 665,018
GFF616
GFF617 - no description, at 665,194 to 665,487
GFF617
GFF618 - Lactoylglutathione lyase and related lyases, at 665,484 to 665,834
GFF618
Position (kb) 662
663
664
665 Strain fitness (log2 ratio) -1
0
1 at 661.395 kb on + strand, within GFF613 at 661.396 kb on - strand, within GFF613 at 661.443 kb on + strand, within GFF613 at 661.443 kb on + strand, within GFF613 at 661.444 kb on - strand, within GFF613 at 661.444 kb on - strand, within GFF613 at 661.446 kb on + strand, within GFF613 at 661.446 kb on + strand, within GFF613 at 661.447 kb on - strand, within GFF613 at 661.548 kb on - strand, within GFF613 at 661.605 kb on + strand, within GFF613 at 662.090 kb on - strand at 662.095 kb on + strand at 662.095 kb on + strand at 662.095 kb on + strand at 662.095 kb on + strand at 662.095 kb on + strand at 662.095 kb on + strand at 662.095 kb on + strand at 662.095 kb on + strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.096 kb on - strand at 662.296 kb on - strand at 662.350 kb on - strand at 663.039 kb on + strand, within GFF615 at 663.066 kb on + strand, within GFF615 at 663.109 kb on - strand, within GFF615 at 663.264 kb on + strand, within GFF615 at 663.372 kb on + strand, within GFF615 at 663.372 kb on + strand, within GFF615 at 663.372 kb on + strand, within GFF615 at 663.372 kb on + strand, within GFF615 at 663.372 kb on + strand, within GFF615 at 663.373 kb on - strand, within GFF615 at 663.373 kb on - strand, within GFF615 at 663.373 kb on - strand, within GFF615 at 663.382 kb on - strand, within GFF615 at 664.161 kb on + strand, within GFF615 at 664.161 kb on + strand, within GFF615 at 664.161 kb on + strand, within GFF615 at 664.161 kb on + strand, within GFF615 at 664.162 kb on - strand, within GFF615 at 664.162 kb on - strand, within GFF615 at 664.162 kb on - strand, within GFF615 at 664.165 kb on - strand, within GFF615 at 664.296 kb on + strand, within GFF615 at 664.407 kb on + strand, within GFF615 at 664.408 kb on - strand, within GFF615 at 664.408 kb on - strand, within GFF615 at 665.034 kb on + strand at 665.041 kb on - strand at 665.041 kb on - strand at 665.049 kb on + strand at 665.050 kb on - strand at 665.059 kb on - strand at 665.068 kb on + strand at 665.068 kb on + strand at 665.068 kb on + strand at 665.072 kb on + strand at 665.072 kb on + strand at 665.073 kb on - strand at 665.073 kb on - strand at 665.094 kb on + strand at 665.094 kb on + strand at 665.157 kb on + strand at 665.157 kb on + strand at 665.158 kb on - strand at 665.216 kb on + strand at 665.217 kb on - strand at 665.217 kb on - strand at 665.260 kb on + strand, within GFF617 at 665.261 kb on - strand, within GFF617 at 665.261 kb on - strand, within GFF617 at 665.396 kb on + strand, within GFF617 at 665.547 kb on + strand, within GFF618
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 661,395 + GFF613 0.25 +0.6 661,396 - GFF613 0.26 +0.2 661,443 + GFF613 0.30 -0.5 661,443 + GFF613 0.30 +1.5 661,444 - GFF613 0.31 -0.2 661,444 - GFF613 0.31 -0.7 661,446 + GFF613 0.31 -1.6 661,446 + GFF613 0.31 -0.5 661,447 - GFF613 0.31 -0.9 661,548 - GFF613 0.41 +0.5 661,605 + GFF613 0.47 +0.4 662,090 - -0.6 662,095 + -0.4 662,095 + +0.8 662,095 + +1.2 662,095 + +0.5 662,095 + -0.3 662,095 + +0.3 662,095 + +0.1 662,095 + -0.6 662,096 - +0.2 662,096 - +0.7 662,096 - -0.3 662,096 - +0.6 662,096 - -0.0 662,096 - -0.4 662,096 - +0.5 662,096 - +0.3 662,096 - +0.1 662,096 - -0.2 662,096 - -0.8 662,096 - -0.9 662,296 - +0.4 662,350 - +0.2 663,039 + GFF615 0.30 +0.3 663,066 + GFF615 0.31 -0.4 663,109 - GFF615 0.33 +0.0 663,264 + GFF615 0.40 +0.3 663,372 + GFF615 0.44 -0.6 663,372 + GFF615 0.44 +0.2 663,372 + GFF615 0.44 -0.2 663,372 + GFF615 0.44 -0.6 663,372 + GFF615 0.44 +0.6 663,373 - GFF615 0.44 -0.6 663,373 - GFF615 0.44 -0.9 663,373 - GFF615 0.44 +0.5 663,382 - GFF615 0.45 -0.1 664,161 + GFF615 0.79 +1.0 664,161 + GFF615 0.79 -0.6 664,161 + GFF615 0.79 +0.7 664,161 + GFF615 0.79 -0.7 664,162 - GFF615 0.79 +1.4 664,162 - GFF615 0.79 -0.0 664,162 - GFF615 0.79 -0.2 664,165 - GFF615 0.79 -0.0 664,296 + GFF615 0.85 +1.1 664,407 + GFF615 0.90 -0.3 664,408 - GFF615 0.90 -0.1 664,408 - GFF615 0.90 -0.4 665,034 + -0.8 665,041 - -0.3 665,041 - +0.2 665,049 + -1.1 665,050 - -0.9 665,059 - +1.1 665,068 + -0.7 665,068 + -0.2 665,068 + -0.4 665,072 + +0.0 665,072 + +0.0 665,073 - +0.1 665,073 - -0.1 665,094 + -0.1 665,094 + +0.2 665,157 + -0.9 665,157 + +0.0 665,158 - +0.8 665,216 + -1.1 665,217 - -0.6 665,217 - -0.1 665,260 + GFF617 0.22 -0.1 665,261 - GFF617 0.23 +0.3 665,261 - GFF617 0.23 +1.0 665,396 + GFF617 0.69 -0.4 665,547 + GFF618 0.18 -0.9
Or see this region's nucleotide sequence