Strain Fitness in Variovorax sp. SCN45 around GFF5979

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5977 and GFF5978 are separated by 56 nucleotidesGFF5978 and GFF5979 are separated by 367 nucleotidesGFF5979 and GFF5980 are separated by 1 nucleotidesGFF5980 and GFF5981 are separated by 48 nucleotidesGFF5981 and GFF5982 are separated by 91 nucleotides GFF5977 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23), at 32,173 to 33,060 GFF5977 GFF5978 - Nucleoside 2-deoxyribosyltransferase, at 33,117 to 33,677 GFF5978 GFF5979 - Short-chain dehydrogenase/reductase SDR?, at 34,045 to 34,806 GFF5979 GFF5980 - Cyanate hydratase (EC 4.2.1.104), at 34,808 to 35,251 GFF5980 GFF5981 - COGs COG3558, at 35,300 to 35,713 GFF5981 GFF5982 - Transcriptional regulator, AcrR family, at 35,805 to 36,395 GFF5982 Position (kb) 34 35Strain fitness (log2 ratio) -2 -1 0 1 2at 33.093 kb on - strandat 33.331 kb on + strand, within GFF5978at 33.331 kb on + strand, within GFF5978at 33.331 kb on + strand, within GFF5978at 33.332 kb on - strand, within GFF5978at 33.332 kb on - strand, within GFF5978at 33.332 kb on - strand, within GFF5978at 33.352 kb on + strand, within GFF5978at 33.490 kb on + strand, within GFF5978at 33.490 kb on + strand, within GFF5978at 33.490 kb on + strand, within GFF5978at 33.490 kb on + strand, within GFF5978at 33.490 kb on + strand, within GFF5978at 33.490 kb on + strand, within GFF5978at 33.491 kb on - strand, within GFF5978at 33.491 kb on - strand, within GFF5978at 33.637 kb on + strandat 33.637 kb on + strandat 33.637 kb on + strandat 33.637 kb on + strandat 33.638 kb on - strandat 33.638 kb on - strandat 33.693 kb on + strandat 33.693 kb on + strandat 33.693 kb on + strandat 33.693 kb on + strandat 33.693 kb on + strandat 33.693 kb on + strandat 33.694 kb on - strandat 33.694 kb on - strandat 33.694 kb on - strandat 33.698 kb on - strandat 33.698 kb on - strandat 33.851 kb on + strandat 34.067 kb on + strandat 34.068 kb on - strandat 34.507 kb on + strand, within GFF5979at 34.507 kb on + strand, within GFF5979at 34.700 kb on + strand, within GFF5979at 34.732 kb on + strandat 34.795 kb on + strandat 34.796 kb on - strandat 34.796 kb on - strandat 34.812 kb on + strandat 34.812 kb on + strandat 34.812 kb on + strandat 34.813 kb on - strandat 34.813 kb on - strandat 34.813 kb on - strandat 34.821 kb on + strandat 34.822 kb on - strandat 34.965 kb on + strand, within GFF5980at 34.966 kb on - strand, within GFF5980at 34.984 kb on - strand, within GFF5980at 34.984 kb on - strand, within GFF5980at 34.993 kb on - strand, within GFF5980at 35.037 kb on + strand, within GFF5980at 35.037 kb on + strand, within GFF5980at 35.038 kb on - strand, within GFF5980at 35.038 kb on - strand, within GFF5980at 35.038 kb on - strand, within GFF5980at 35.601 kb on + strand, within GFF5981at 35.602 kb on - strand, within GFF5981at 35.713 kb on + strandat 35.793 kb on + strandat 35.794 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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33,093 - +0.4
33,331 + GFF5978 0.38 +0.2
33,331 + GFF5978 0.38 -0.0
33,331 + GFF5978 0.38 +0.3
33,332 - GFF5978 0.38 +0.5
33,332 - GFF5978 0.38 +0.2
33,332 - GFF5978 0.38 +0.4
33,352 + GFF5978 0.42 +1.3
33,490 + GFF5978 0.66 -0.1
33,490 + GFF5978 0.66 -0.3
33,490 + GFF5978 0.66 +0.4
33,490 + GFF5978 0.66 +0.6
33,490 + GFF5978 0.66 +0.2
33,490 + GFF5978 0.66 +0.2
33,491 - GFF5978 0.67 +0.4
33,491 - GFF5978 0.67 -0.6
33,637 + -0.4
33,637 + -1.0
33,637 + +0.2
33,637 + +0.6
33,638 - +0.4
33,638 - -0.3
33,693 + -0.7
33,693 + +2.6
33,693 + -0.4
33,693 + -0.8
33,693 + -0.4
33,693 + -0.6
33,694 - +0.4
33,694 - +0.0
33,694 - -2.0
33,698 - +0.0
33,698 - -1.0
33,851 + +0.6
34,067 + -0.2
34,068 - +0.4
34,507 + GFF5979 0.61 +0.6
34,507 + GFF5979 0.61 -0.7
34,700 + GFF5979 0.86 +0.6
34,732 + -1.2
34,795 + -0.4
34,796 - -0.6
34,796 - -0.7
34,812 + +0.6
34,812 + -1.3
34,812 + +0.2
34,813 - -0.3
34,813 - -0.6
34,813 - +2.2
34,821 + -0.3
34,822 - -0.5
34,965 + GFF5980 0.35 -0.7
34,966 - GFF5980 0.36 -0.4
34,984 - GFF5980 0.40 -0.3
34,984 - GFF5980 0.40 -0.7
34,993 - GFF5980 0.42 -0.3
35,037 + GFF5980 0.52 -1.0
35,037 + GFF5980 0.52 +1.6
35,038 - GFF5980 0.52 +1.6
35,038 - GFF5980 0.52 -1.1
35,038 - GFF5980 0.52 -0.3
35,601 + GFF5981 0.73 -0.3
35,602 - GFF5981 0.73 -0.4
35,713 + -0.7
35,793 + +1.2
35,794 - -0.1

Or see this region's nucleotide sequence