Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5977 and GFF5978 are separated by 56 nucleotides GFF5978 and GFF5979 are separated by 367 nucleotides GFF5979 and GFF5980 are separated by 1 nucleotides GFF5980 and GFF5981 are separated by 48 nucleotides GFF5981 and GFF5982 are separated by 91 nucleotides
GFF5977 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23), at 32,173 to 33,060
GFF5977
GFF5978 - Nucleoside 2-deoxyribosyltransferase, at 33,117 to 33,677
GFF5978
GFF5979 - Short-chain dehydrogenase/reductase SDR?, at 34,045 to 34,806
GFF5979
GFF5980 - Cyanate hydratase (EC 4.2.1.104), at 34,808 to 35,251
GFF5980
GFF5981 - COGs COG3558, at 35,300 to 35,713
GFF5981
GFF5982 - Transcriptional regulator, AcrR family, at 35,805 to 36,395
GFF5982
Position (kb)
34
35 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 33.093 kb on - strand at 33.331 kb on + strand, within GFF5978 at 33.331 kb on + strand, within GFF5978 at 33.331 kb on + strand, within GFF5978 at 33.332 kb on - strand, within GFF5978 at 33.332 kb on - strand, within GFF5978 at 33.332 kb on - strand, within GFF5978 at 33.352 kb on + strand, within GFF5978 at 33.490 kb on + strand, within GFF5978 at 33.490 kb on + strand, within GFF5978 at 33.490 kb on + strand, within GFF5978 at 33.490 kb on + strand, within GFF5978 at 33.490 kb on + strand, within GFF5978 at 33.490 kb on + strand, within GFF5978 at 33.491 kb on - strand, within GFF5978 at 33.491 kb on - strand, within GFF5978 at 33.637 kb on + strand at 33.637 kb on + strand at 33.637 kb on + strand at 33.637 kb on + strand at 33.638 kb on - strand at 33.638 kb on - strand at 33.693 kb on + strand at 33.693 kb on + strand at 33.693 kb on + strand at 33.693 kb on + strand at 33.693 kb on + strand at 33.693 kb on + strand at 33.694 kb on - strand at 33.694 kb on - strand at 33.694 kb on - strand at 33.698 kb on - strand at 33.698 kb on - strand at 33.851 kb on + strand at 34.067 kb on + strand at 34.068 kb on - strand at 34.507 kb on + strand, within GFF5979 at 34.507 kb on + strand, within GFF5979 at 34.700 kb on + strand, within GFF5979 at 34.732 kb on + strand at 34.795 kb on + strand at 34.796 kb on - strand at 34.796 kb on - strand at 34.812 kb on + strand at 34.812 kb on + strand at 34.812 kb on + strand at 34.813 kb on - strand at 34.813 kb on - strand at 34.813 kb on - strand at 34.821 kb on + strand at 34.822 kb on - strand at 34.965 kb on + strand, within GFF5980 at 34.966 kb on - strand, within GFF5980 at 34.984 kb on - strand, within GFF5980 at 34.984 kb on - strand, within GFF5980 at 34.993 kb on - strand, within GFF5980 at 35.037 kb on + strand, within GFF5980 at 35.037 kb on + strand, within GFF5980 at 35.038 kb on - strand, within GFF5980 at 35.038 kb on - strand, within GFF5980 at 35.038 kb on - strand, within GFF5980 at 35.601 kb on + strand, within GFF5981 at 35.602 kb on - strand, within GFF5981 at 35.713 kb on + strand at 35.793 kb on + strand at 35.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 33,093 - +0.4 33,331 + GFF5978 0.38 +0.2 33,331 + GFF5978 0.38 -0.0 33,331 + GFF5978 0.38 +0.3 33,332 - GFF5978 0.38 +0.5 33,332 - GFF5978 0.38 +0.2 33,332 - GFF5978 0.38 +0.4 33,352 + GFF5978 0.42 +1.3 33,490 + GFF5978 0.66 -0.1 33,490 + GFF5978 0.66 -0.3 33,490 + GFF5978 0.66 +0.4 33,490 + GFF5978 0.66 +0.6 33,490 + GFF5978 0.66 +0.2 33,490 + GFF5978 0.66 +0.2 33,491 - GFF5978 0.67 +0.4 33,491 - GFF5978 0.67 -0.6 33,637 + -0.4 33,637 + -1.0 33,637 + +0.2 33,637 + +0.6 33,638 - +0.4 33,638 - -0.3 33,693 + -0.7 33,693 + +2.6 33,693 + -0.4 33,693 + -0.8 33,693 + -0.4 33,693 + -0.6 33,694 - +0.4 33,694 - +0.0 33,694 - -2.0 33,698 - +0.0 33,698 - -1.0 33,851 + +0.6 34,067 + -0.2 34,068 - +0.4 34,507 + GFF5979 0.61 +0.6 34,507 + GFF5979 0.61 -0.7 34,700 + GFF5979 0.86 +0.6 34,732 + -1.2 34,795 + -0.4 34,796 - -0.6 34,796 - -0.7 34,812 + +0.6 34,812 + -1.3 34,812 + +0.2 34,813 - -0.3 34,813 - -0.6 34,813 - +2.2 34,821 + -0.3 34,822 - -0.5 34,965 + GFF5980 0.35 -0.7 34,966 - GFF5980 0.36 -0.4 34,984 - GFF5980 0.40 -0.3 34,984 - GFF5980 0.40 -0.7 34,993 - GFF5980 0.42 -0.3 35,037 + GFF5980 0.52 -1.0 35,037 + GFF5980 0.52 +1.6 35,038 - GFF5980 0.52 +1.6 35,038 - GFF5980 0.52 -1.1 35,038 - GFF5980 0.52 -0.3 35,601 + GFF5981 0.73 -0.3 35,602 - GFF5981 0.73 -0.4 35,713 + -0.7 35,793 + +1.2 35,794 - -0.1
Or see this region's nucleotide sequence