Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5889 and GFF5890 overlap by 4 nucleotides GFF5890 and GFF5891 are separated by 31 nucleotides GFF5891 and GFF5892 are separated by 21 nucleotides GFF5892 and GFF5893 are separated by 17 nucleotides
GFF5889 - Uracil-DNA glycosylase, family 1 (EC 3.2.2.27), at 108,872 to 109,579
GFF5889
GFF5890 - Indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 109,576 to 110,376
GFF5890
GFF5891 - Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 110,408 to 111,430
GFF5891
GFF5892 - Lysine exporter protein LysE/YggA, at 111,452 to 112,111
GFF5892
GFF5893 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 112,129 to 113,187
GFF5893
Position (kb) 110
111
112 Strain fitness (log2 ratio) -2
-1
0
1 at 110.377 kb on - strand at 110.377 kb on - strand at 111.864 kb on + strand, within GFF5892 at 111.864 kb on + strand, within GFF5892 at 111.879 kb on + strand, within GFF5892 at 111.879 kb on + strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 111.880 kb on - strand, within GFF5892 at 112.032 kb on + strand, within GFF5892 at 112.032 kb on + strand, within GFF5892 at 112.032 kb on + strand, within GFF5892 at 112.032 kb on + strand, within GFF5892 at 112.033 kb on - strand, within GFF5892 at 112.217 kb on + strand at 112.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 110,377 - +0.3 110,377 - +0.7 111,864 + GFF5892 0.62 +1.3 111,864 + GFF5892 0.62 -0.3 111,879 + GFF5892 0.65 +0.5 111,879 + GFF5892 0.65 -0.8 111,880 - GFF5892 0.65 +1.1 111,880 - GFF5892 0.65 +0.4 111,880 - GFF5892 0.65 -0.3 111,880 - GFF5892 0.65 +1.1 111,880 - GFF5892 0.65 +0.2 111,880 - GFF5892 0.65 +0.3 111,880 - GFF5892 0.65 +0.3 112,032 + GFF5892 0.88 -0.8 112,032 + GFF5892 0.88 -0.8 112,032 + GFF5892 0.88 +0.5 112,032 + GFF5892 0.88 +0.7 112,033 - GFF5892 0.88 -2.3 112,217 + -0.4 112,218 - +1.3
Or see this region's nucleotide sequence