Strain Fitness in Variovorax sp. SCN45 around GFF5881

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5880 and GFF5881 are separated by 21 nucleotidesGFF5881 and GFF5882 are separated by 250 nucleotidesGFF5882 and GFF5883 are separated by 41 nucleotides GFF5880 - DNA-directed RNA polymerase beta' subunit (EC 2.7.7.6), at 94,841 to 99,076 GFF5880 GFF5881 - DNA-directed RNA polymerase beta subunit (EC 2.7.7.6), at 99,098 to 103,222 GFF5881 GFF5882 - LSU ribosomal protein L7p/L12p (P1/P2), at 103,473 to 103,847 GFF5882 GFF5883 - LSU ribosomal protein L10p (P0), at 103,889 to 104,428 GFF5883 Position (kb) 99 100 101 102 103 104Strain fitness (log2 ratio) -1 0 1at 103.461 kb on - strandat 103.461 kb on - strandat 103.474 kb on + strandat 103.474 kb on + strandat 103.475 kb on - strandat 103.475 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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103,461 - -0.0
103,461 - -1.1
103,474 + +1.7
103,474 + +0.2
103,475 - -1.6
103,475 - -0.9

Or see this region's nucleotide sequence