Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5844 and GFF5845 are separated by 25 nucleotides GFF5845 and GFF5846 are separated by 87 nucleotides GFF5846 and GFF5847 are separated by 1 nucleotides
GFF5844 - Phospholipase A1 (EC 3.1.1.32) (EC 3.1.1.4) @ Outer membrane phospholipase A, at 21,233 to 22,399
GFF5844
GFF5845 - NAD synthetase (EC 6.3.1.5), at 22,425 to 23,282
GFF5845
GFF5846 - FIG00453548: hypothetical protein, at 23,370 to 24,089
GFF5846
GFF5847 - putative membrane protein, at 24,091 to 26,745
GFF5847
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-1
0
1 at 22.409 kb on + strand at 22.409 kb on - strand at 22.545 kb on + strand, within GFF5845 at 22.545 kb on + strand, within GFF5845 at 22.545 kb on + strand, within GFF5845 at 22.545 kb on + strand, within GFF5845 at 22.545 kb on + strand, within GFF5845 at 22.545 kb on + strand, within GFF5845 at 22.546 kb on - strand, within GFF5845 at 22.546 kb on - strand, within GFF5845 at 22.546 kb on - strand, within GFF5845 at 22.693 kb on - strand, within GFF5845 at 22.693 kb on - strand, within GFF5845 at 23.073 kb on + strand, within GFF5845 at 23.073 kb on + strand, within GFF5845 at 23.074 kb on - strand, within GFF5845 at 23.074 kb on - strand, within GFF5845 at 23.074 kb on - strand, within GFF5845 at 23.074 kb on - strand, within GFF5845 at 23.074 kb on - strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.340 kb on + strand at 23.340 kb on + strand at 23.340 kb on + strand at 23.341 kb on - strand at 23.348 kb on + strand at 23.349 kb on - strand at 24.160 kb on + strand at 24.161 kb on - strand at 24.214 kb on + strand at 24.215 kb on - strand at 24.409 kb on + strand, within GFF5847 at 24.409 kb on + strand, within GFF5847 at 24.409 kb on + strand, within GFF5847 at 24.410 kb on - strand, within GFF5847 at 24.410 kb on - strand, within GFF5847 at 24.410 kb on - strand, within GFF5847 at 24.871 kb on + strand, within GFF5847 at 24.872 kb on - strand, within GFF5847 at 24.872 kb on - strand, within GFF5847 at 24.872 kb on - strand, within GFF5847 at 24.872 kb on - strand, within GFF5847
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 22,409 + +0.2 22,409 - +1.5 22,545 + GFF5845 0.14 +0.3 22,545 + GFF5845 0.14 -0.2 22,545 + GFF5845 0.14 +1.1 22,545 + GFF5845 0.14 -0.4 22,545 + GFF5845 0.14 +1.0 22,545 + GFF5845 0.14 -0.1 22,546 - GFF5845 0.14 +0.0 22,546 - GFF5845 0.14 -0.1 22,546 - GFF5845 0.14 +0.9 22,693 - GFF5845 0.31 +0.2 22,693 - GFF5845 0.31 +1.3 23,073 + GFF5845 0.76 -0.4 23,073 + GFF5845 0.76 -1.0 23,074 - GFF5845 0.76 +0.7 23,074 - GFF5845 0.76 -1.4 23,074 - GFF5845 0.76 +1.7 23,074 - GFF5845 0.76 -0.5 23,074 - GFF5845 0.76 -0.6 23,145 + GFF5845 0.84 -0.6 23,145 + GFF5845 0.84 +0.3 23,145 + GFF5845 0.84 +0.0 23,145 + GFF5845 0.84 -0.9 23,145 + GFF5845 0.84 +0.0 23,146 - GFF5845 0.84 -0.5 23,146 - GFF5845 0.84 -0.2 23,146 - GFF5845 0.84 +0.3 23,146 - GFF5845 0.84 -0.7 23,146 - GFF5845 0.84 +0.4 23,340 + -0.9 23,340 + +0.1 23,340 + +1.7 23,341 - +0.2 23,348 + +0.6 23,349 - -0.4 24,160 + +0.0 24,161 - +0.1 24,214 + -1.6 24,215 - -0.2 24,409 + GFF5847 0.12 -0.4 24,409 + GFF5847 0.12 +0.7 24,409 + GFF5847 0.12 +0.9 24,410 - GFF5847 0.12 +0.1 24,410 - GFF5847 0.12 -0.6 24,410 - GFF5847 0.12 -0.8 24,871 + GFF5847 0.29 -0.7 24,872 - GFF5847 0.29 -0.1 24,872 - GFF5847 0.29 +0.4 24,872 - GFF5847 0.29 -0.2 24,872 - GFF5847 0.29 +0.1
Or see this region's nucleotide sequence