Strain Fitness in Variovorax sp. SCN45 around GFF5835

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5834 and GFF5835 are separated by 140 nucleotidesGFF5835 and GFF5836 are separated by 26 nucleotidesGFF5836 and GFF5837 are separated by 106 nucleotides GFF5834 - Oxidoreductase, GMC family, at 9,924 to 11,525 GFF5834 GFF5835 - Uroporphyrinogen III decarboxylase (EC 4.1.1.37), at 11,666 to 12,775 GFF5835 GFF5836 - no description, at 12,802 to 13,038 GFF5836 GFF5837 - Helicase PriA essential for oriC/DnaA-independent DNA replication, at 13,145 to 15,325 GFF5837 Position (kb) 11 12 13Strain fitness (log2 ratio) -2 -1 0 1at 10.828 kb on + strand, within GFF5834at 10.829 kb on - strand, within GFF5834at 10.829 kb on - strand, within GFF5834at 10.927 kb on + strand, within GFF5834at 10.927 kb on + strand, within GFF5834at 10.927 kb on + strand, within GFF5834at 10.927 kb on + strand, within GFF5834at 10.928 kb on - strand, within GFF5834at 10.928 kb on - strand, within GFF5834at 10.928 kb on - strand, within GFF5834at 10.928 kb on - strand, within GFF5834at 10.928 kb on - strand, within GFF5834at 10.975 kb on + strand, within GFF5834at 10.975 kb on + strand, within GFF5834at 10.975 kb on + strand, within GFF5834at 10.975 kb on + strand, within GFF5834at 10.976 kb on - strand, within GFF5834at 10.976 kb on - strand, within GFF5834at 10.976 kb on - strand, within GFF5834at 10.976 kb on - strand, within GFF5834at 11.020 kb on + strand, within GFF5834at 11.021 kb on - strand, within GFF5834at 11.219 kb on - strand, within GFF5834at 11.242 kb on + strand, within GFF5834at 11.242 kb on + strand, within GFF5834at 11.242 kb on + strand, within GFF5834at 11.242 kb on + strand, within GFF5834at 11.242 kb on + strand, within GFF5834at 11.243 kb on - strand, within GFF5834at 11.243 kb on - strand, within GFF5834at 11.243 kb on - strand, within GFF5834at 11.243 kb on - strand, within GFF5834at 11.243 kb on - strand, within GFF5834at 11.243 kb on - strand, within GFF5834at 11.243 kb on - strand, within GFF5834at 11.243 kb on - strand, within GFF5834at 11.339 kb on - strand, within GFF5834at 11.339 kb on - strand, within GFF5834at 11.339 kb on - strand, within GFF5834at 12.861 kb on + strand, within GFF5836at 12.861 kb on + strand, within GFF5836at 12.861 kb on + strand, within GFF5836at 12.861 kb on + strand, within GFF5836at 12.861 kb on + strand, within GFF5836at 12.862 kb on - strand, within GFF5836at 12.862 kb on - strand, within GFF5836at 12.862 kb on - strand, within GFF5836at 12.862 kb on - strand, within GFF5836at 12.862 kb on - strand, within GFF5836at 12.862 kb on - strand, within GFF5836at 12.862 kb on - strand, within GFF5836at 12.862 kb on - strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.874 kb on + strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.875 kb on - strand, within GFF5836at 12.952 kb on - strand, within GFF5836at 12.966 kb on + strand, within GFF5836at 12.967 kb on - strand, within GFF5836at 12.969 kb on - strand, within GFF5836at 13.036 kb on + strandat 13.036 kb on + strandat 13.036 kb on + strandat 13.036 kb on + strandat 13.037 kb on - strandat 13.062 kb on - strandat 13.111 kb on + strandat 13.111 kb on + strandat 13.112 kb on - strandat 13.112 kb on - strandat 13.112 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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10,828 + GFF5834 0.56 +0.3
10,829 - GFF5834 0.56 -0.0
10,829 - GFF5834 0.56 -0.5
10,927 + GFF5834 0.63 +0.1
10,927 + GFF5834 0.63 -0.9
10,927 + GFF5834 0.63 -1.1
10,927 + GFF5834 0.63 +0.3
10,928 - GFF5834 0.63 +0.4
10,928 - GFF5834 0.63 -1.1
10,928 - GFF5834 0.63 -0.0
10,928 - GFF5834 0.63 -0.2
10,928 - GFF5834 0.63 -0.4
10,975 + GFF5834 0.66 +1.7
10,975 + GFF5834 0.66 -0.7
10,975 + GFF5834 0.66 +0.0
10,975 + GFF5834 0.66 +0.3
10,976 - GFF5834 0.66 +1.2
10,976 - GFF5834 0.66 +1.2
10,976 - GFF5834 0.66 -0.7
10,976 - GFF5834 0.66 +1.0
11,020 + GFF5834 0.68 -0.4
11,021 - GFF5834 0.68 -0.8
11,219 - GFF5834 0.81 -1.4
11,242 + GFF5834 0.82 +0.3
11,242 + GFF5834 0.82 -0.2
11,242 + GFF5834 0.82 -0.8
11,242 + GFF5834 0.82 -1.1
11,242 + GFF5834 0.82 -0.1
11,243 - GFF5834 0.82 -0.3
11,243 - GFF5834 0.82 +0.7
11,243 - GFF5834 0.82 -0.0
11,243 - GFF5834 0.82 -0.0
11,243 - GFF5834 0.82 -0.3
11,243 - GFF5834 0.82 +0.2
11,243 - GFF5834 0.82 +0.8
11,243 - GFF5834 0.82 -0.3
11,339 - GFF5834 0.88 -0.7
11,339 - GFF5834 0.88 +0.3
11,339 - GFF5834 0.88 -2.2
12,861 + GFF5836 0.25 -1.3
12,861 + GFF5836 0.25 -0.7
12,861 + GFF5836 0.25 -0.9
12,861 + GFF5836 0.25 -0.6
12,861 + GFF5836 0.25 +0.0
12,862 - GFF5836 0.25 +0.2
12,862 - GFF5836 0.25 -2.0
12,862 - GFF5836 0.25 -0.3
12,862 - GFF5836 0.25 -0.1
12,862 - GFF5836 0.25 +0.2
12,862 - GFF5836 0.25 -0.0
12,862 - GFF5836 0.25 -1.0
12,862 - GFF5836 0.25 -1.5
12,874 + GFF5836 0.30 -0.8
12,874 + GFF5836 0.30 +0.3
12,874 + GFF5836 0.30 -0.7
12,874 + GFF5836 0.30 +0.2
12,874 + GFF5836 0.30 +0.2
12,874 + GFF5836 0.30 +0.8
12,874 + GFF5836 0.30 -0.4
12,874 + GFF5836 0.30 -0.3
12,874 + GFF5836 0.30 +1.3
12,874 + GFF5836 0.30 +1.2
12,874 + GFF5836 0.30 +0.4
12,875 - GFF5836 0.31 -2.3
12,875 - GFF5836 0.31 -0.1
12,875 - GFF5836 0.31 -0.5
12,875 - GFF5836 0.31 +0.1
12,875 - GFF5836 0.31 -0.1
12,875 - GFF5836 0.31 -0.3
12,875 - GFF5836 0.31 +1.3
12,875 - GFF5836 0.31 +0.4
12,875 - GFF5836 0.31 +0.7
12,875 - GFF5836 0.31 -0.2
12,875 - GFF5836 0.31 +0.3
12,875 - GFF5836 0.31 -0.5
12,952 - GFF5836 0.63 +0.3
12,966 + GFF5836 0.69 -0.9
12,967 - GFF5836 0.70 -0.3
12,969 - GFF5836 0.70 -0.1
13,036 + +0.0
13,036 + +1.3
13,036 + +0.7
13,036 + -0.3
13,037 - +0.5
13,062 - +0.2
13,111 + -0.3
13,111 + -0.7
13,112 - -1.1
13,112 - +0.5
13,112 - -0.2

Or see this region's nucleotide sequence