Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5824 and GFF5825 are separated by 20 nucleotides GFF5825 and GFF5826 are separated by 10 nucleotides GFF5826 and GFF5827 overlap by 4 nucleotides
GFF5824 - no description, at 1,236 to 1,538
GFF5824
GFF5825 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 1,559 to 2,266
GFF5825
GFF5826 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,277 to 2,984
GFF5826
GFF5827 - FIG00452956: hypothetical protein, at 2,981 to 4,054
GFF5827
Position (kb)
2
3 Strain fitness (log2 ratio)
-2
-1
0
1 at 1.536 kb on + strand at 1.537 kb on - strand at 1.537 kb on - strand at 1.575 kb on + strand at 1.575 kb on + strand at 1.589 kb on + strand at 1.589 kb on + strand at 1.590 kb on - strand at 1.590 kb on - strand at 1.656 kb on + strand, within GFF5825 at 1.656 kb on + strand, within GFF5825 at 1.657 kb on - strand, within GFF5825 at 1.657 kb on - strand, within GFF5825 at 1.657 kb on - strand, within GFF5825 at 1.994 kb on + strand, within GFF5825 at 1.994 kb on + strand, within GFF5825 at 2.294 kb on - strand at 2.294 kb on - strand at 3.120 kb on + strand, within GFF5827 at 3.121 kb on - strand, within GFF5827 at 3.121 kb on - strand, within GFF5827 at 3.201 kb on + strand, within GFF5827 at 3.201 kb on + strand, within GFF5827 at 3.201 kb on + strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.489 kb on + strand, within GFF5827 at 3.490 kb on - strand, within GFF5827 at 3.490 kb on - strand, within GFF5827 at 3.490 kb on - strand, within GFF5827 at 3.645 kb on + strand, within GFF5827 at 3.645 kb on + strand, within GFF5827 at 3.645 kb on + strand, within GFF5827 at 3.646 kb on - strand, within GFF5827 at 3.646 kb on - strand, within GFF5827 at 3.795 kb on + strand, within GFF5827 at 3.795 kb on + strand, within GFF5827
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 1,536 + -0.3 1,537 - +0.1 1,537 - -1.1 1,575 + +0.0 1,575 + -0.7 1,589 + -0.4 1,589 + -0.4 1,590 - +0.4 1,590 - -0.5 1,656 + GFF5825 0.14 -1.1 1,656 + GFF5825 0.14 -0.4 1,657 - GFF5825 0.14 +0.4 1,657 - GFF5825 0.14 -0.4 1,657 - GFF5825 0.14 +0.5 1,994 + GFF5825 0.61 +0.2 1,994 + GFF5825 0.61 +0.7 2,294 - -1.1 2,294 - +0.5 3,120 + GFF5827 0.13 -0.4 3,121 - GFF5827 0.13 -0.4 3,121 - GFF5827 0.13 -2.3 3,201 + GFF5827 0.20 +0.1 3,201 + GFF5827 0.20 +0.5 3,201 + GFF5827 0.20 -1.1 3,202 - GFF5827 0.21 -0.7 3,202 - GFF5827 0.21 -0.3 3,202 - GFF5827 0.21 -0.3 3,202 - GFF5827 0.21 +0.1 3,202 - GFF5827 0.21 +0.3 3,202 - GFF5827 0.21 -0.0 3,489 + GFF5827 0.47 +0.5 3,490 - GFF5827 0.47 +0.3 3,490 - GFF5827 0.47 -0.1 3,490 - GFF5827 0.47 +0.5 3,645 + GFF5827 0.62 +0.4 3,645 + GFF5827 0.62 -0.3 3,645 + GFF5827 0.62 -0.3 3,646 - GFF5827 0.62 -0.6 3,646 - GFF5827 0.62 -0.5 3,795 + GFF5827 0.76 +0.3 3,795 + GFF5827 0.76 +0.9
Or see this region's nucleotide sequence