Strain Fitness in Variovorax sp. SCN45 around GFF579

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF578 and GFF579 are separated by 25 nucleotidesGFF579 and GFF580 are separated by 62 nucleotidesGFF580 and GFF581 are separated by 22 nucleotides GFF578 - Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129), at 622,389 to 624,119 GFF578 GFF579 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 624,145 to 627,663 GFF579 GFF580 - Rhodanese domain protein UPF0176, Betaproteobacterial subgroup, at 627,726 to 628,517 GFF580 GFF581 - Tricarboxylate transport transcriptional regulator TctD, at 628,540 to 629,250 GFF581 Position (kb) 624 625 626 627 628Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 623.217 kb on + strand, within GFF578at 623.217 kb on + strand, within GFF578at 623.217 kb on + strand, within GFF578at 623.217 kb on + strand, within GFF578at 623.218 kb on - strand, within GFF578at 623.218 kb on - strand, within GFF578at 623.218 kb on - strand, within GFF578at 623.226 kb on + strand, within GFF578at 623.226 kb on + strand, within GFF578at 623.226 kb on + strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.227 kb on - strand, within GFF578at 623.640 kb on + strand, within GFF578at 623.640 kb on + strand, within GFF578at 623.640 kb on + strand, within GFF578at 623.640 kb on + strand, within GFF578at 623.641 kb on - strand, within GFF578at 623.641 kb on - strand, within GFF578at 623.685 kb on + strand, within GFF578at 623.685 kb on + strand, within GFF578at 623.685 kb on + strand, within GFF578at 623.686 kb on - strand, within GFF578at 623.686 kb on - strand, within GFF578at 623.686 kb on - strand, within GFF578at 623.686 kb on - strand, within GFF578at 623.859 kb on + strand, within GFF578at 623.859 kb on + strand, within GFF578at 623.911 kb on - strand, within GFF578at 623.925 kb on + strand, within GFF578at 623.925 kb on + strand, within GFF578at 623.925 kb on + strand, within GFF578at 623.925 kb on + strand, within GFF578at 623.925 kb on + strand, within GFF578at 623.926 kb on - strand, within GFF578at 623.926 kb on - strand, within GFF578at 623.937 kb on + strand, within GFF578at 623.937 kb on + strand, within GFF578at 623.937 kb on + strand, within GFF578at 623.937 kb on + strand, within GFF578at 623.938 kb on - strand, within GFF578at 623.938 kb on - strand, within GFF578at 623.938 kb on - strand, within GFF578at 624.012 kb on + strandat 624.012 kb on + strandat 624.012 kb on + strandat 624.013 kb on - strandat 624.013 kb on - strandat 624.013 kb on - strandat 624.013 kb on - strandat 624.013 kb on - strandat 627.674 kb on + strandat 627.674 kb on + strandat 627.674 kb on + strandat 627.674 kb on + strandat 627.674 kb on + strandat 627.674 kb on + strandat 627.674 kb on + strandat 627.675 kb on - strandat 627.675 kb on - strandat 627.675 kb on - strandat 627.675 kb on - strandat 627.712 kb on + strandat 627.712 kb on + strandat 627.712 kb on + strandat 627.712 kb on + strandat 627.712 kb on + strandat 627.712 kb on + strandat 627.713 kb on - strandat 627.713 kb on - strandat 627.713 kb on - strandat 627.713 kb on - strandat 627.743 kb on + strandat 627.743 kb on + strandat 627.753 kb on + strandat 627.753 kb on + strandat 627.753 kb on + strandat 627.754 kb on - strandat 627.858 kb on - strand, within GFF580at 627.858 kb on - strand, within GFF580at 627.892 kb on - strand, within GFF580at 627.893 kb on - strand, within GFF580at 628.365 kb on + strand, within GFF580at 628.366 kb on - strand, within GFF580at 628.366 kb on - strand, within GFF580at 628.392 kb on + strand, within GFF580at 628.392 kb on + strand, within GFF580at 628.393 kb on - strand, within GFF580at 628.393 kb on - strand, within GFF580

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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623,217 + GFF578 0.48 +0.0
623,217 + GFF578 0.48 -0.3
623,217 + GFF578 0.48 -0.7
623,217 + GFF578 0.48 +0.2
623,218 - GFF578 0.48 -0.7
623,218 - GFF578 0.48 +1.2
623,218 - GFF578 0.48 +0.2
623,226 + GFF578 0.48 -0.5
623,226 + GFF578 0.48 +0.5
623,226 + GFF578 0.48 +0.2
623,227 - GFF578 0.48 -0.2
623,227 - GFF578 0.48 +0.1
623,227 - GFF578 0.48 +0.5
623,227 - GFF578 0.48 -0.4
623,227 - GFF578 0.48 -0.0
623,227 - GFF578 0.48 -0.4
623,227 - GFF578 0.48 -0.9
623,227 - GFF578 0.48 -0.0
623,227 - GFF578 0.48 +0.5
623,227 - GFF578 0.48 -0.4
623,227 - GFF578 0.48 -1.1
623,227 - GFF578 0.48 -0.0
623,227 - GFF578 0.48 -1.4
623,640 + GFF578 0.72 -0.1
623,640 + GFF578 0.72 -1.0
623,640 + GFF578 0.72 +0.3
623,640 + GFF578 0.72 +0.6
623,641 - GFF578 0.72 -0.3
623,641 - GFF578 0.72 -1.0
623,685 + GFF578 0.75 -0.0
623,685 + GFF578 0.75 -0.4
623,685 + GFF578 0.75 -0.2
623,686 - GFF578 0.75 +0.7
623,686 - GFF578 0.75 -0.9
623,686 - GFF578 0.75 -1.3
623,686 - GFF578 0.75 +0.3
623,859 + GFF578 0.85 -0.6
623,859 + GFF578 0.85 -0.1
623,911 - GFF578 0.88 +0.1
623,925 + GFF578 0.89 +0.2
623,925 + GFF578 0.89 +0.6
623,925 + GFF578 0.89 -0.5
623,925 + GFF578 0.89 -0.8
623,925 + GFF578 0.89 +0.3
623,926 - GFF578 0.89 +0.2
623,926 - GFF578 0.89 -0.4
623,937 + GFF578 0.89 -1.1
623,937 + GFF578 0.89 -0.2
623,937 + GFF578 0.89 -0.5
623,937 + GFF578 0.89 -0.1
623,938 - GFF578 0.89 -1.8
623,938 - GFF578 0.89 -0.9
623,938 - GFF578 0.89 +0.2
624,012 + -0.7
624,012 + -1.6
624,012 + +0.2
624,013 - +0.3
624,013 - -0.6
624,013 - -0.2
624,013 - +0.5
624,013 - +0.7
627,674 + +0.2
627,674 + -0.3
627,674 + +1.2
627,674 + -0.4
627,674 + +0.4
627,674 + -0.1
627,674 + +0.8
627,675 - +2.5
627,675 - +1.2
627,675 - -0.1
627,675 - +0.7
627,712 + +1.2
627,712 + -0.1
627,712 + -0.0
627,712 + +0.8
627,712 + +0.5
627,712 + -1.1
627,713 - -0.6
627,713 - -0.2
627,713 - -0.8
627,713 - -0.5
627,743 + -0.6
627,743 + -0.1
627,753 + +0.2
627,753 + -0.4
627,753 + +1.4
627,754 - -2.9
627,858 - GFF580 0.17 -0.1
627,858 - GFF580 0.17 +1.8
627,892 - GFF580 0.21 -0.5
627,893 - GFF580 0.21 -1.8
628,365 + GFF580 0.81 -0.4
628,366 - GFF580 0.81 -0.1
628,366 - GFF580 0.81 -1.4
628,392 + GFF580 0.84 -0.7
628,392 + GFF580 0.84 -1.9
628,393 - GFF580 0.84 -0.6
628,393 - GFF580 0.84 +0.1

Or see this region's nucleotide sequence