Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5743 and GFF5744 are separated by 27 nucleotides GFF5744 and GFF5745 are separated by 154 nucleotides GFF5745 and GFF5746 are separated by 16 nucleotides GFF5746 and GFF5747 overlap by 4 nucleotides
GFF5743 - Flagellar motor switch protein FliM, at 115,754 to 116,758
GFF5743
GFF5744 - Flagellar basal body-associated protein FliL, at 116,786 to 117,289
GFF5744
GFF5745 - Flagellar hook-length control protein FliK, at 117,444 to 118,883
GFF5745
GFF5746 - Flagellar protein FliJ, at 118,900 to 119,355
GFF5746
GFF5747 - Flagellum-specific ATP synthase FliI, at 119,352 to 120,821
GFF5747
Position (kb) 117
118
119 Strain fitness (log2 ratio) -1
0
1
2 at 116.752 kb on - strand at 117.284 kb on - strand at 117.315 kb on + strand at 117.315 kb on + strand at 118.220 kb on + strand, within GFF5745 at 119.210 kb on + strand, within GFF5746 at 119.211 kb on - strand, within GFF5746 at 119.222 kb on + strand, within GFF5746 at 119.506 kb on + strand, within GFF5747 at 119.506 kb on + strand, within GFF5747 at 119.692 kb on + strand, within GFF5747 at 119.803 kb on + strand, within GFF5747 at 119.803 kb on + strand, within GFF5747 at 119.804 kb on - strand, within GFF5747
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 116,752 - -0.4 117,284 - -0.3 117,315 + +0.3 117,315 + -0.4 118,220 + GFF5745 0.54 +0.0 119,210 + GFF5746 0.68 -1.0 119,211 - GFF5746 0.68 +0.1 119,222 + GFF5746 0.71 +1.8 119,506 + GFF5747 0.10 -0.2 119,506 + GFF5747 0.10 -0.6 119,692 + GFF5747 0.23 -0.4 119,803 + GFF5747 0.31 -1.1 119,803 + GFF5747 0.31 -0.3 119,804 - GFF5747 0.31 +0.0
Or see this region's nucleotide sequence