Strain Fitness in Variovorax sp. SCN45 around GFF5744

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5743 and GFF5744 are separated by 27 nucleotidesGFF5744 and GFF5745 are separated by 154 nucleotides GFF5743 - Flagellar motor switch protein FliM, at 115,754 to 116,758 GFF5743 GFF5744 - Flagellar basal body-associated protein FliL, at 116,786 to 117,289 GFF5744 GFF5745 - Flagellar hook-length control protein FliK, at 117,444 to 118,883 GFF5745 Position (kb) 116 117 118Strain fitness (log2 ratio) -1 0 1at 115.819 kb on - strandat 116.215 kb on - strand, within GFF5743at 116.215 kb on - strand, within GFF5743at 116.340 kb on + strand, within GFF5743at 116.341 kb on - strand, within GFF5743at 116.752 kb on - strandat 117.284 kb on - strandat 117.315 kb on + strandat 117.315 kb on + strandat 118.220 kb on + strand, within GFF5745

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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115,819 - +0.1
116,215 - GFF5743 0.46 +0.0
116,215 - GFF5743 0.46 -0.1
116,340 + GFF5743 0.58 -0.7
116,341 - GFF5743 0.58 +0.1
116,752 - -0.4
117,284 - -0.3
117,315 + +0.3
117,315 + -0.4
118,220 + GFF5745 0.54 +0.0

Or see this region's nucleotide sequence