Strain Fitness in Variovorax sp. SCN45 around GFF5741

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5739 and GFF5740 are separated by 11 nucleotidesGFF5740 and GFF5741 overlap by 4 nucleotidesGFF5741 and GFF5742 overlap by 4 nucleotidesGFF5742 and GFF5743 overlap by 8 nucleotides GFF5739 - Flagellar biosynthesis protein FliQ, at 113,755 to 114,024 GFF5739 GFF5740 - Flagellar biosynthesis protein FliP, at 114,036 to 114,794 GFF5740 GFF5741 - Flagellar biosynthesis protein FliO, at 114,791 to 115,294 GFF5741 GFF5742 - Flagellar motor switch protein FliN, at 115,291 to 115,761 GFF5742 GFF5743 - Flagellar motor switch protein FliM, at 115,754 to 116,758 GFF5743 Position (kb) 114 115 116Strain fitness (log2 ratio) -2 -1 0 1at 113.798 kb on + strand, within GFF5739at 113.799 kb on - strand, within GFF5739at 114.038 kb on - strandat 114.041 kb on + strandat 114.041 kb on + strandat 114.041 kb on + strandat 114.042 kb on - strandat 114.043 kb on + strandat 114.043 kb on + strandat 114.043 kb on + strandat 114.043 kb on + strandat 114.043 kb on + strandat 114.043 kb on + strandat 114.044 kb on - strandat 114.044 kb on - strandat 114.044 kb on - strandat 114.044 kb on - strandat 114.044 kb on - strandat 114.437 kb on - strand, within GFF5740at 114.448 kb on + strand, within GFF5740at 115.355 kb on + strand, within GFF5742at 115.356 kb on - strand, within GFF5742at 115.356 kb on - strand, within GFF5742at 115.397 kb on + strand, within GFF5742at 115.819 kb on - strandat 116.215 kb on - strand, within GFF5743at 116.215 kb on - strand, within GFF5743

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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113,798 + GFF5739 0.16 +1.0
113,799 - GFF5739 0.16 +0.1
114,038 - +1.6
114,041 + -0.4
114,041 + -0.6
114,041 + -0.7
114,042 - -0.5
114,043 + -0.1
114,043 + +0.5
114,043 + -0.7
114,043 + +0.0
114,043 + +0.4
114,043 + -1.2
114,044 - +1.2
114,044 - +0.1
114,044 - -1.7
114,044 - +1.3
114,044 - +1.2
114,437 - GFF5740 0.53 +0.3
114,448 + GFF5740 0.54 -2.1
115,355 + GFF5742 0.14 -0.8
115,356 - GFF5742 0.14 +1.1
115,356 - GFF5742 0.14 +0.0
115,397 + GFF5742 0.23 -1.2
115,819 - +0.1
116,215 - GFF5743 0.46 +0.0
116,215 - GFF5743 0.46 -0.1

Or see this region's nucleotide sequence