Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5739 and GFF5740 are separated by 11 nucleotides GFF5740 and GFF5741 overlap by 4 nucleotides GFF5741 and GFF5742 overlap by 4 nucleotides GFF5742 and GFF5743 overlap by 8 nucleotides
GFF5739 - Flagellar biosynthesis protein FliQ, at 113,755 to 114,024
GFF5739
GFF5740 - Flagellar biosynthesis protein FliP, at 114,036 to 114,794
GFF5740
GFF5741 - Flagellar biosynthesis protein FliO, at 114,791 to 115,294
GFF5741
GFF5742 - Flagellar motor switch protein FliN, at 115,291 to 115,761
GFF5742
GFF5743 - Flagellar motor switch protein FliM, at 115,754 to 116,758
GFF5743
Position (kb)
114
115
116 Strain fitness (log2 ratio)
-2
-1
0
1 at 113.798 kb on + strand, within GFF5739 at 113.799 kb on - strand, within GFF5739 at 114.038 kb on - strand at 114.041 kb on + strand at 114.041 kb on + strand at 114.041 kb on + strand at 114.042 kb on - strand at 114.043 kb on + strand at 114.043 kb on + strand at 114.043 kb on + strand at 114.043 kb on + strand at 114.043 kb on + strand at 114.043 kb on + strand at 114.044 kb on - strand at 114.044 kb on - strand at 114.044 kb on - strand at 114.044 kb on - strand at 114.044 kb on - strand at 114.437 kb on - strand, within GFF5740 at 114.448 kb on + strand, within GFF5740 at 115.355 kb on + strand, within GFF5742 at 115.356 kb on - strand, within GFF5742 at 115.356 kb on - strand, within GFF5742 at 115.397 kb on + strand, within GFF5742 at 115.819 kb on - strand at 116.215 kb on - strand, within GFF5743 at 116.215 kb on - strand, within GFF5743
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 113,798 + GFF5739 0.16 +1.0 113,799 - GFF5739 0.16 +0.1 114,038 - +1.6 114,041 + -0.4 114,041 + -0.6 114,041 + -0.7 114,042 - -0.5 114,043 + -0.1 114,043 + +0.5 114,043 + -0.7 114,043 + +0.0 114,043 + +0.4 114,043 + -1.2 114,044 - +1.2 114,044 - +0.1 114,044 - -1.7 114,044 - +1.3 114,044 - +1.2 114,437 - GFF5740 0.53 +0.3 114,448 + GFF5740 0.54 -2.1 115,355 + GFF5742 0.14 -0.8 115,356 - GFF5742 0.14 +1.1 115,356 - GFF5742 0.14 +0.0 115,397 + GFF5742 0.23 -1.2 115,819 - +0.1 116,215 - GFF5743 0.46 +0.0 116,215 - GFF5743 0.46 -0.1
Or see this region's nucleotide sequence