Strain Fitness in Variovorax sp. SCN45 around GFF5704

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5702 and GFF5703 overlap by 4 nucleotidesGFF5703 and GFF5704 are separated by 23 nucleotidesGFF5704 and GFF5705 are separated by 231 nucleotidesGFF5705 and GFF5706 are separated by 3 nucleotides GFF5702 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.7.4), at 75,939 to 76,907 GFF5702 GFF5703 - pyridoxal-phosphate dependent enzyme family protein, at 76,904 to 77,869 GFF5703 GFF5704 - Seryl-tRNA synthetase (EC 6.1.1.11), at 77,893 to 79,224 GFF5704 GFF5705 - Transcriptional regulator, AcrR family, at 79,456 to 80,031 GFF5705 GFF5706 - no description, at 80,035 to 80,187 GFF5706 Position (kb) 77 78 79 80Strain fitness (log2 ratio) -2 -1 0 1at 76.941 kb on + strandat 76.942 kb on - strandat 76.945 kb on + strandat 76.945 kb on + strandat 76.945 kb on + strandat 76.945 kb on + strandat 77.306 kb on + strand, within GFF5703at 77.306 kb on + strand, within GFF5703at 77.306 kb on + strand, within GFF5703at 77.306 kb on + strand, within GFF5703at 77.306 kb on + strand, within GFF5703at 77.307 kb on - strand, within GFF5703at 77.372 kb on + strand, within GFF5703at 77.372 kb on + strand, within GFF5703at 77.373 kb on - strand, within GFF5703at 77.373 kb on - strand, within GFF5703at 77.373 kb on - strand, within GFF5703at 77.373 kb on - strand, within GFF5703at 77.373 kb on - strand, within GFF5703at 77.795 kb on + strandat 77.795 kb on + strandat 77.795 kb on + strandat 77.795 kb on + strandat 77.795 kb on + strandat 77.795 kb on + strandat 77.796 kb on - strandat 77.796 kb on - strandat 77.796 kb on - strandat 77.796 kb on - strandat 77.796 kb on - strandat 77.796 kb on - strandat 77.796 kb on - strandat 77.796 kb on - strandat 77.881 kb on + strandat 79.242 kb on + strandat 79.444 kb on + strandat 79.444 kb on + strandat 79.444 kb on + strandat 79.444 kb on + strandat 79.444 kb on + strandat 79.445 kb on - strandat 79.445 kb on - strandat 79.445 kb on - strandat 79.445 kb on - strandat 79.482 kb on - strandat 79.482 kb on - strandat 79.539 kb on + strand, within GFF5705at 79.539 kb on + strand, within GFF5705at 79.539 kb on + strand, within GFF5705at 79.539 kb on + strand, within GFF5705at 79.539 kb on + strand, within GFF5705at 79.539 kb on + strand, within GFF5705at 79.539 kb on + strand, within GFF5705at 79.540 kb on - strand, within GFF5705at 79.540 kb on - strand, within GFF5705at 79.540 kb on - strand, within GFF5705at 79.540 kb on - strand, within GFF5705at 79.540 kb on - strand, within GFF5705at 79.540 kb on - strand, within GFF5705at 79.573 kb on + strand, within GFF5705at 79.573 kb on + strand, within GFF5705at 79.573 kb on + strand, within GFF5705at 79.574 kb on - strand, within GFF5705at 79.574 kb on - strand, within GFF5705at 79.601 kb on - strand, within GFF5705at 79.621 kb on + strand, within GFF5705at 79.621 kb on + strand, within GFF5705at 79.621 kb on + strand, within GFF5705at 79.621 kb on + strand, within GFF5705at 79.621 kb on + strand, within GFF5705at 79.622 kb on - strand, within GFF5705at 79.622 kb on - strand, within GFF5705at 79.622 kb on - strand, within GFF5705at 79.622 kb on - strand, within GFF5705at 79.622 kb on - strand, within GFF5705at 79.622 kb on - strand, within GFF5705at 79.834 kb on + strand, within GFF5705at 79.835 kb on - strand, within GFF5705at 79.835 kb on - strand, within GFF5705at 79.835 kb on - strand, within GFF5705at 80.144 kb on + strand, within GFF5706at 80.144 kb on + strand, within GFF5706at 80.144 kb on + strand, within GFF5706at 80.144 kb on - strand, within GFF5706at 80.145 kb on - strand, within GFF5706at 80.145 kb on - strand, within GFF5706at 80.153 kb on + strand, within GFF5706

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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76,941 + -0.2
76,942 - +0.0
76,945 + -0.7
76,945 + -0.4
76,945 + -0.3
76,945 + -1.2
77,306 + GFF5703 0.42 +0.9
77,306 + GFF5703 0.42 -1.9
77,306 + GFF5703 0.42 +0.4
77,306 + GFF5703 0.42 +0.1
77,306 + GFF5703 0.42 -0.3
77,307 - GFF5703 0.42 -1.3
77,372 + GFF5703 0.48 +0.1
77,372 + GFF5703 0.48 -1.2
77,373 - GFF5703 0.49 -0.0
77,373 - GFF5703 0.49 +1.7
77,373 - GFF5703 0.49 -0.8
77,373 - GFF5703 0.49 +0.2
77,373 - GFF5703 0.49 +0.1
77,795 + -0.1
77,795 + -0.4
77,795 + +0.8
77,795 + -1.0
77,795 + -0.8
77,795 + -0.9
77,796 - +0.5
77,796 - +0.4
77,796 - -0.8
77,796 - -0.1
77,796 - +0.9
77,796 - -0.0
77,796 - +0.3
77,796 - -1.3
77,881 + -0.2
79,242 + -0.3
79,444 + +0.0
79,444 + -0.5
79,444 + +0.0
79,444 + +0.6
79,444 + +0.4
79,445 - +1.0
79,445 - -0.3
79,445 - +1.2
79,445 - -0.2
79,482 - +0.4
79,482 - -0.9
79,539 + GFF5705 0.14 +0.1
79,539 + GFF5705 0.14 -0.8
79,539 + GFF5705 0.14 -0.1
79,539 + GFF5705 0.14 -0.4
79,539 + GFF5705 0.14 +0.3
79,539 + GFF5705 0.14 +0.0
79,539 + GFF5705 0.14 -0.4
79,540 - GFF5705 0.15 +0.1
79,540 - GFF5705 0.15 +0.0
79,540 - GFF5705 0.15 +0.4
79,540 - GFF5705 0.15 -0.3
79,540 - GFF5705 0.15 +1.2
79,540 - GFF5705 0.15 -0.0
79,573 + GFF5705 0.20 -1.6
79,573 + GFF5705 0.20 -1.6
79,573 + GFF5705 0.20 -0.6
79,574 - GFF5705 0.20 -1.4
79,574 - GFF5705 0.20 +0.0
79,601 - GFF5705 0.25 -0.2
79,621 + GFF5705 0.29 -0.5
79,621 + GFF5705 0.29 -1.0
79,621 + GFF5705 0.29 -2.3
79,621 + GFF5705 0.29 -0.2
79,621 + GFF5705 0.29 +0.4
79,622 - GFF5705 0.29 -0.2
79,622 - GFF5705 0.29 -0.2
79,622 - GFF5705 0.29 +0.6
79,622 - GFF5705 0.29 -0.1
79,622 - GFF5705 0.29 +0.4
79,622 - GFF5705 0.29 -0.5
79,834 + GFF5705 0.66 -0.4
79,835 - GFF5705 0.66 +1.0
79,835 - GFF5705 0.66 +0.2
79,835 - GFF5705 0.66 +1.0
80,144 + GFF5706 0.71 -0.7
80,144 + GFF5706 0.71 +1.0
80,144 + GFF5706 0.71 +0.2
80,144 - GFF5706 0.71 +0.5
80,145 - GFF5706 0.72 -0.1
80,145 - GFF5706 0.72 +0.9
80,153 + GFF5706 0.77 +1.0

Or see this region's nucleotide sequence