Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5702 and GFF5703 overlap by 4 nucleotides GFF5703 and GFF5704 are separated by 23 nucleotides GFF5704 and GFF5705 are separated by 231 nucleotides GFF5705 and GFF5706 are separated by 3 nucleotides
GFF5702 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.7.4), at 75,939 to 76,907
GFF5702
GFF5703 - pyridoxal-phosphate dependent enzyme family protein, at 76,904 to 77,869
GFF5703
GFF5704 - Seryl-tRNA synthetase (EC 6.1.1.11), at 77,893 to 79,224
GFF5704
GFF5705 - Transcriptional regulator, AcrR family, at 79,456 to 80,031
GFF5705
GFF5706 - no description, at 80,035 to 80,187
GFF5706
Position (kb)
77
78
79
80 Strain fitness (log2 ratio)
-2
-1
0
1 at 76.941 kb on + strand at 76.942 kb on - strand at 76.945 kb on + strand at 76.945 kb on + strand at 76.945 kb on + strand at 76.945 kb on + strand at 77.306 kb on + strand, within GFF5703 at 77.306 kb on + strand, within GFF5703 at 77.306 kb on + strand, within GFF5703 at 77.306 kb on + strand, within GFF5703 at 77.306 kb on + strand, within GFF5703 at 77.307 kb on - strand, within GFF5703 at 77.372 kb on + strand, within GFF5703 at 77.372 kb on + strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.795 kb on + strand at 77.795 kb on + strand at 77.795 kb on + strand at 77.795 kb on + strand at 77.795 kb on + strand at 77.795 kb on + strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.881 kb on + strand at 79.242 kb on + strand at 79.444 kb on + strand at 79.444 kb on + strand at 79.444 kb on + strand at 79.444 kb on + strand at 79.444 kb on + strand at 79.445 kb on - strand at 79.445 kb on - strand at 79.445 kb on - strand at 79.445 kb on - strand at 79.482 kb on - strand at 79.482 kb on - strand at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.539 kb on + strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.540 kb on - strand, within GFF5705 at 79.573 kb on + strand, within GFF5705 at 79.573 kb on + strand, within GFF5705 at 79.573 kb on + strand, within GFF5705 at 79.574 kb on - strand, within GFF5705 at 79.574 kb on - strand, within GFF5705 at 79.601 kb on - strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.621 kb on + strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.622 kb on - strand, within GFF5705 at 79.834 kb on + strand, within GFF5705 at 79.835 kb on - strand, within GFF5705 at 79.835 kb on - strand, within GFF5705 at 79.835 kb on - strand, within GFF5705 at 80.144 kb on + strand, within GFF5706 at 80.144 kb on + strand, within GFF5706 at 80.144 kb on + strand, within GFF5706 at 80.144 kb on - strand, within GFF5706 at 80.145 kb on - strand, within GFF5706 at 80.145 kb on - strand, within GFF5706 at 80.153 kb on + strand, within GFF5706
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 76,941 + -0.2 76,942 - +0.0 76,945 + -0.7 76,945 + -0.4 76,945 + -0.3 76,945 + -1.2 77,306 + GFF5703 0.42 +0.9 77,306 + GFF5703 0.42 -1.9 77,306 + GFF5703 0.42 +0.4 77,306 + GFF5703 0.42 +0.1 77,306 + GFF5703 0.42 -0.3 77,307 - GFF5703 0.42 -1.3 77,372 + GFF5703 0.48 +0.1 77,372 + GFF5703 0.48 -1.2 77,373 - GFF5703 0.49 -0.0 77,373 - GFF5703 0.49 +1.7 77,373 - GFF5703 0.49 -0.8 77,373 - GFF5703 0.49 +0.2 77,373 - GFF5703 0.49 +0.1 77,795 + -0.1 77,795 + -0.4 77,795 + +0.8 77,795 + -1.0 77,795 + -0.8 77,795 + -0.9 77,796 - +0.5 77,796 - +0.4 77,796 - -0.8 77,796 - -0.1 77,796 - +0.9 77,796 - -0.0 77,796 - +0.3 77,796 - -1.3 77,881 + -0.2 79,242 + -0.3 79,444 + +0.0 79,444 + -0.5 79,444 + +0.0 79,444 + +0.6 79,444 + +0.4 79,445 - +1.0 79,445 - -0.3 79,445 - +1.2 79,445 - -0.2 79,482 - +0.4 79,482 - -0.9 79,539 + GFF5705 0.14 +0.1 79,539 + GFF5705 0.14 -0.8 79,539 + GFF5705 0.14 -0.1 79,539 + GFF5705 0.14 -0.4 79,539 + GFF5705 0.14 +0.3 79,539 + GFF5705 0.14 +0.0 79,539 + GFF5705 0.14 -0.4 79,540 - GFF5705 0.15 +0.1 79,540 - GFF5705 0.15 +0.0 79,540 - GFF5705 0.15 +0.4 79,540 - GFF5705 0.15 -0.3 79,540 - GFF5705 0.15 +1.2 79,540 - GFF5705 0.15 -0.0 79,573 + GFF5705 0.20 -1.6 79,573 + GFF5705 0.20 -1.6 79,573 + GFF5705 0.20 -0.6 79,574 - GFF5705 0.20 -1.4 79,574 - GFF5705 0.20 +0.0 79,601 - GFF5705 0.25 -0.2 79,621 + GFF5705 0.29 -0.5 79,621 + GFF5705 0.29 -1.0 79,621 + GFF5705 0.29 -2.3 79,621 + GFF5705 0.29 -0.2 79,621 + GFF5705 0.29 +0.4 79,622 - GFF5705 0.29 -0.2 79,622 - GFF5705 0.29 -0.2 79,622 - GFF5705 0.29 +0.6 79,622 - GFF5705 0.29 -0.1 79,622 - GFF5705 0.29 +0.4 79,622 - GFF5705 0.29 -0.5 79,834 + GFF5705 0.66 -0.4 79,835 - GFF5705 0.66 +1.0 79,835 - GFF5705 0.66 +0.2 79,835 - GFF5705 0.66 +1.0 80,144 + GFF5706 0.71 -0.7 80,144 + GFF5706 0.71 +1.0 80,144 + GFF5706 0.71 +0.2 80,144 - GFF5706 0.71 +0.5 80,145 - GFF5706 0.72 -0.1 80,145 - GFF5706 0.72 +0.9 80,153 + GFF5706 0.77 +1.0
Or see this region's nucleotide sequence