Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5695 and GFF5696 are separated by 27 nucleotides GFF5696 and GFF5697 are separated by 50 nucleotides GFF5697 and GFF5698 are separated by 88 nucleotides
GFF5695 - Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9), FabV => refractory to triclosan, at 68,882 to 70,078
GFF5695
GFF5696 - Hemoglobin-like protein HbO, at 70,106 to 70,525
GFF5696
GFF5697 - Ferredoxin reductase, at 70,576 to 71,805
GFF5697
GFF5698 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16) / 3'-nucleotidase (EC 3.1.3.6), at 71,894 to 73,996
GFF5698
Position (kb) 70
71
72 Strain fitness (log2 ratio) -2
-1
0
1
2 at 69.584 kb on + strand, within GFF5695 at 69.584 kb on + strand, within GFF5695 at 69.584 kb on + strand, within GFF5695 at 69.585 kb on - strand, within GFF5695 at 69.585 kb on - strand, within GFF5695 at 69.740 kb on + strand, within GFF5695 at 69.740 kb on + strand, within GFF5695 at 69.740 kb on + strand, within GFF5695 at 69.740 kb on + strand, within GFF5695 at 69.741 kb on - strand, within GFF5695 at 69.741 kb on - strand, within GFF5695 at 69.815 kb on + strand, within GFF5695 at 69.816 kb on - strand, within GFF5695 at 69.816 kb on - strand, within GFF5695 at 69.816 kb on - strand, within GFF5695 at 69.831 kb on - strand, within GFF5695 at 69.878 kb on + strand, within GFF5695 at 69.878 kb on + strand, within GFF5695 at 69.879 kb on - strand, within GFF5695 at 69.879 kb on - strand, within GFF5695 at 69.879 kb on - strand, within GFF5695 at 70.026 kb on - strand at 70.026 kb on - strand at 70.095 kb on + strand at 70.095 kb on + strand at 70.095 kb on + strand at 70.096 kb on - strand at 70.193 kb on + strand, within GFF5696 at 70.193 kb on + strand, within GFF5696 at 70.194 kb on - strand, within GFF5696 at 70.194 kb on - strand, within GFF5696 at 70.194 kb on - strand, within GFF5696 at 70.194 kb on - strand, within GFF5696 at 70.221 kb on - strand, within GFF5696 at 70.316 kb on + strand, within GFF5696 at 70.316 kb on + strand, within GFF5696 at 70.317 kb on - strand, within GFF5696 at 70.317 kb on - strand, within GFF5696 at 70.317 kb on - strand, within GFF5696 at 70.317 kb on - strand, within GFF5696 at 70.547 kb on + strand at 70.548 kb on - strand at 70.551 kb on + strand at 70.551 kb on + strand at 70.551 kb on + strand at 70.551 kb on + strand at 70.551 kb on + strand at 70.552 kb on - strand at 70.552 kb on - strand at 70.583 kb on - strand at 70.693 kb on + strand at 70.694 kb on - strand at 71.804 kb on - strand at 71.804 kb on - strand at 71.804 kb on - strand at 72.125 kb on + strand, within GFF5698 at 72.125 kb on + strand, within GFF5698 at 72.152 kb on + strand, within GFF5698 at 72.152 kb on + strand, within GFF5698 at 72.153 kb on - strand, within GFF5698 at 72.153 kb on - strand, within GFF5698 at 72.158 kb on + strand, within GFF5698 at 72.158 kb on + strand, within GFF5698 at 72.260 kb on + strand, within GFF5698 at 72.260 kb on + strand, within GFF5698 at 72.261 kb on - strand, within GFF5698 at 72.261 kb on - strand, within GFF5698 at 72.261 kb on - strand, within GFF5698 at 72.261 kb on - strand, within GFF5698 at 72.261 kb on - strand, within GFF5698 at 72.308 kb on + strand, within GFF5698 at 72.309 kb on - strand, within GFF5698 at 72.369 kb on - strand, within GFF5698 at 72.369 kb on - strand, within GFF5698 at 72.369 kb on - strand, within GFF5698 at 72.516 kb on - strand, within GFF5698 at 72.516 kb on - strand, within GFF5698 at 72.639 kb on + strand, within GFF5698 at 72.640 kb on - strand, within GFF5698 at 72.640 kb on - strand, within GFF5698 at 72.686 kb on + strand, within GFF5698 at 72.686 kb on + strand, within GFF5698 at 72.687 kb on - strand, within GFF5698 at 72.791 kb on + strand, within GFF5698 at 72.791 kb on + strand, within GFF5698 at 72.792 kb on - strand, within GFF5698 at 72.792 kb on - strand, within GFF5698 at 72.792 kb on - strand, within GFF5698
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 69,584 + GFF5695 0.59 +0.2 69,584 + GFF5695 0.59 +0.3 69,584 + GFF5695 0.59 -1.2 69,585 - GFF5695 0.59 -1.0 69,585 - GFF5695 0.59 -0.8 69,740 + GFF5695 0.72 +0.0 69,740 + GFF5695 0.72 +0.4 69,740 + GFF5695 0.72 +0.0 69,740 + GFF5695 0.72 -0.3 69,741 - GFF5695 0.72 -0.7 69,741 - GFF5695 0.72 +0.2 69,815 + GFF5695 0.78 +0.6 69,816 - GFF5695 0.78 -0.7 69,816 - GFF5695 0.78 -0.4 69,816 - GFF5695 0.78 +0.2 69,831 - GFF5695 0.79 -0.4 69,878 + GFF5695 0.83 +0.6 69,878 + GFF5695 0.83 -1.6 69,879 - GFF5695 0.83 +0.0 69,879 - GFF5695 0.83 +0.5 69,879 - GFF5695 0.83 +0.1 70,026 - +1.0 70,026 - +0.0 70,095 + -2.0 70,095 + -0.3 70,095 + -2.7 70,096 - +0.1 70,193 + GFF5696 0.21 -0.7 70,193 + GFF5696 0.21 -0.7 70,194 - GFF5696 0.21 -0.5 70,194 - GFF5696 0.21 -0.1 70,194 - GFF5696 0.21 -0.9 70,194 - GFF5696 0.21 +0.1 70,221 - GFF5696 0.27 -0.8 70,316 + GFF5696 0.50 -0.1 70,316 + GFF5696 0.50 -1.2 70,317 - GFF5696 0.50 -0.1 70,317 - GFF5696 0.50 +0.2 70,317 - GFF5696 0.50 -0.4 70,317 - GFF5696 0.50 -0.1 70,547 + -0.3 70,548 - +0.2 70,551 + +0.9 70,551 + -0.2 70,551 + +0.9 70,551 + -0.0 70,551 + +0.2 70,552 - -0.3 70,552 - +0.0 70,583 - -2.1 70,693 + -0.1 70,694 - +0.9 71,804 - +0.8 71,804 - -0.4 71,804 - +1.6 72,125 + GFF5698 0.11 -0.3 72,125 + GFF5698 0.11 -0.3 72,152 + GFF5698 0.12 -1.0 72,152 + GFF5698 0.12 +0.8 72,153 - GFF5698 0.12 +1.6 72,153 - GFF5698 0.12 -0.2 72,158 + GFF5698 0.13 -0.2 72,158 + GFF5698 0.13 +2.2 72,260 + GFF5698 0.17 +0.0 72,260 + GFF5698 0.17 +0.0 72,261 - GFF5698 0.17 -0.4 72,261 - GFF5698 0.17 -0.2 72,261 - GFF5698 0.17 -0.1 72,261 - GFF5698 0.17 -0.1 72,261 - GFF5698 0.17 -0.2 72,308 + GFF5698 0.20 -0.0 72,309 - GFF5698 0.20 +0.3 72,369 - GFF5698 0.23 +0.3 72,369 - GFF5698 0.23 -0.3 72,369 - GFF5698 0.23 +0.3 72,516 - GFF5698 0.30 +0.2 72,516 - GFF5698 0.30 +2.6 72,639 + GFF5698 0.35 +0.1 72,640 - GFF5698 0.35 +0.8 72,640 - GFF5698 0.35 +0.0 72,686 + GFF5698 0.38 +0.5 72,686 + GFF5698 0.38 -0.7 72,687 - GFF5698 0.38 -0.1 72,791 + GFF5698 0.43 -0.2 72,791 + GFF5698 0.43 -0.5 72,792 - GFF5698 0.43 +0.0 72,792 - GFF5698 0.43 +1.4 72,792 - GFF5698 0.43 -1.3
Or see this region's nucleotide sequence