Strain Fitness in Variovorax sp. SCN45 around GFF5658

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5656 and GFF5657 are separated by 13 nucleotidesGFF5657 and GFF5658 are separated by 1 nucleotidesGFF5658 and GFF5659 are separated by 1 nucleotides GFF5656 - Formate dehydrogenase O gamma subunit (EC 1.2.1.2), at 32,592 to 33,215 GFF5656 GFF5657 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 33,229 to 34,191 GFF5657 GFF5658 - no description, at 34,193 to 34,354 GFF5658 GFF5659 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 34,356 to 36,797 GFF5659 Position (kb) 34 35Strain fitness (log2 ratio) -2 -1 0 1 2at 33.202 kb on + strandat 33.202 kb on + strandat 33.202 kb on + strandat 33.202 kb on + strandat 33.203 kb on - strandat 33.205 kb on + strandat 33.205 kb on + strandat 33.205 kb on + strandat 33.206 kb on - strandat 33.206 kb on - strandat 33.206 kb on - strandat 33.340 kb on + strand, within GFF5657at 33.359 kb on + strand, within GFF5657at 33.359 kb on + strand, within GFF5657at 33.360 kb on - strand, within GFF5657at 33.360 kb on - strand, within GFF5657at 33.476 kb on + strand, within GFF5657at 33.476 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.509 kb on + strand, within GFF5657at 33.510 kb on - strand, within GFF5657at 33.510 kb on - strand, within GFF5657at 33.510 kb on - strand, within GFF5657at 33.510 kb on - strand, within GFF5657at 33.510 kb on - strand, within GFF5657at 33.510 kb on - strand, within GFF5657at 33.545 kb on + strand, within GFF5657at 33.546 kb on - strand, within GFF5657at 33.546 kb on - strand, within GFF5657at 33.546 kb on - strand, within GFF5657at 33.546 kb on - strand, within GFF5657at 33.602 kb on + strand, within GFF5657at 33.603 kb on - strand, within GFF5657at 33.713 kb on + strand, within GFF5657at 33.714 kb on - strand, within GFF5657at 33.714 kb on - strand, within GFF5657at 33.714 kb on - strand, within GFF5657at 33.714 kb on - strand, within GFF5657at 33.714 kb on - strand, within GFF5657at 33.714 kb on - strand, within GFF5657at 33.714 kb on - strand, within GFF5657at 33.824 kb on + strand, within GFF5657at 33.825 kb on - strand, within GFF5657at 33.825 kb on - strand, within GFF5657at 33.825 kb on - strand, within GFF5657at 33.992 kb on + strand, within GFF5657at 33.992 kb on + strand, within GFF5657at 33.992 kb on + strand, within GFF5657at 33.992 kb on + strand, within GFF5657at 34.126 kb on + strandat 34.127 kb on - strandat 34.171 kb on + strandat 34.390 kb on + strandat 34.390 kb on + strandat 34.390 kb on + strandat 34.390 kb on + strandat 34.390 kb on + strandat 34.390 kb on + strandat 34.391 kb on - strandat 34.391 kb on - strandat 34.567 kb on + strandat 34.568 kb on - strandat 34.568 kb on - strandat 34.568 kb on - strandat 34.696 kb on + strand, within GFF5659at 34.697 kb on - strand, within GFF5659at 34.697 kb on - strand, within GFF5659at 34.697 kb on - strand, within GFF5659at 34.697 kb on - strand, within GFF5659at 34.720 kb on + strand, within GFF5659at 34.735 kb on + strand, within GFF5659at 34.735 kb on + strand, within GFF5659at 34.864 kb on + strand, within GFF5659at 34.864 kb on + strand, within GFF5659at 34.865 kb on - strand, within GFF5659at 34.865 kb on - strand, within GFF5659at 34.865 kb on - strand, within GFF5659at 34.865 kb on - strand, within GFF5659at 35.090 kb on - strand, within GFF5659at 35.090 kb on - strand, within GFF5659at 35.209 kb on + strand, within GFF5659at 35.209 kb on + strand, within GFF5659at 35.209 kb on + strand, within GFF5659at 35.209 kb on + strand, within GFF5659at 35.344 kb on + strand, within GFF5659at 35.344 kb on + strand, within GFF5659at 35.344 kb on + strand, within GFF5659at 35.345 kb on - strand, within GFF5659at 35.345 kb on - strand, within GFF5659at 35.345 kb on - strand, within GFF5659

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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33,202 + +0.0
33,202 + +0.2
33,202 + -0.9
33,202 + -0.5
33,203 - +0.2
33,205 + -0.3
33,205 + -0.3
33,205 + -0.6
33,206 - +0.1
33,206 - +1.0
33,206 - +0.1
33,340 + GFF5657 0.12 -0.2
33,359 + GFF5657 0.13 -0.9
33,359 + GFF5657 0.13 -0.7
33,360 - GFF5657 0.14 +0.0
33,360 - GFF5657 0.14 -0.4
33,476 + GFF5657 0.26 -0.4
33,476 + GFF5657 0.26 +1.1
33,509 + GFF5657 0.29 -0.2
33,509 + GFF5657 0.29 -0.3
33,509 + GFF5657 0.29 -0.2
33,509 + GFF5657 0.29 -0.9
33,509 + GFF5657 0.29 -2.0
33,509 + GFF5657 0.29 +0.0
33,509 + GFF5657 0.29 -0.5
33,509 + GFF5657 0.29 -0.7
33,509 + GFF5657 0.29 -0.3
33,509 + GFF5657 0.29 -0.4
33,510 - GFF5657 0.29 -0.0
33,510 - GFF5657 0.29 -0.8
33,510 - GFF5657 0.29 -0.2
33,510 - GFF5657 0.29 -1.3
33,510 - GFF5657 0.29 +0.5
33,510 - GFF5657 0.29 -2.1
33,545 + GFF5657 0.33 +0.8
33,546 - GFF5657 0.33 +0.8
33,546 - GFF5657 0.33 +0.3
33,546 - GFF5657 0.33 -0.4
33,546 - GFF5657 0.33 -0.0
33,602 + GFF5657 0.39 -0.2
33,603 - GFF5657 0.39 +1.0
33,713 + GFF5657 0.50 -0.4
33,714 - GFF5657 0.50 +0.3
33,714 - GFF5657 0.50 +0.9
33,714 - GFF5657 0.50 -0.1
33,714 - GFF5657 0.50 -0.7
33,714 - GFF5657 0.50 -0.3
33,714 - GFF5657 0.50 +1.6
33,714 - GFF5657 0.50 +0.2
33,824 + GFF5657 0.62 +0.2
33,825 - GFF5657 0.62 +0.0
33,825 - GFF5657 0.62 -0.0
33,825 - GFF5657 0.62 +0.2
33,992 + GFF5657 0.79 -0.4
33,992 + GFF5657 0.79 -0.8
33,992 + GFF5657 0.79 +0.2
33,992 + GFF5657 0.79 +0.6
34,126 + +0.0
34,127 - -0.2
34,171 + -0.3
34,390 + -0.1
34,390 + +0.3
34,390 + -1.1
34,390 + +0.1
34,390 + -2.0
34,390 + -0.3
34,391 - -0.2
34,391 - -0.1
34,567 + -0.8
34,568 - -0.5
34,568 - +0.3
34,568 - -0.6
34,696 + GFF5659 0.14 -0.3
34,697 - GFF5659 0.14 -0.4
34,697 - GFF5659 0.14 -0.7
34,697 - GFF5659 0.14 +0.3
34,697 - GFF5659 0.14 -0.9
34,720 + GFF5659 0.15 -1.0
34,735 + GFF5659 0.16 -1.4
34,735 + GFF5659 0.16 -1.1
34,864 + GFF5659 0.21 +0.4
34,864 + GFF5659 0.21 -0.3
34,865 - GFF5659 0.21 -1.5
34,865 - GFF5659 0.21 +2.2
34,865 - GFF5659 0.21 +0.1
34,865 - GFF5659 0.21 -0.5
35,090 - GFF5659 0.30 -0.6
35,090 - GFF5659 0.30 +0.6
35,209 + GFF5659 0.35 -0.6
35,209 + GFF5659 0.35 -0.5
35,209 + GFF5659 0.35 +0.4
35,209 + GFF5659 0.35 -0.3
35,344 + GFF5659 0.40 +0.2
35,344 + GFF5659 0.40 -0.9
35,344 + GFF5659 0.40 +0.0
35,345 - GFF5659 0.40 -0.1
35,345 - GFF5659 0.40 +0.7
35,345 - GFF5659 0.40 -1.1

Or see this region's nucleotide sequence