Strain Fitness in Variovorax sp. SCN45 around GFF5571

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5570 and GFF5571 are separated by 92 nucleotidesGFF5571 and GFF5572 are separated by 218 nucleotidesGFF5572 and GFF5573 are separated by 110 nucleotides GFF5570 - DNA-directed RNA polymerase alpha subunit (EC 2.7.7.6), at 190,939 to 191,928 GFF5570 GFF5571 - LSU ribosomal protein L17p, at 192,021 to 192,419 GFF5571 GFF5572 - FIG00984604: hypothetical protein, at 192,638 to 193,288 GFF5572 GFF5573 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 193,399 to 194,373 GFF5573 Position (kb) 192 193Strain fitness (log2 ratio) -2 -1 0 1 2at 191.983 kb on + strandat 192.417 kb on + strandat 192.417 kb on + strandat 192.518 kb on + strandat 192.620 kb on + strandat 192.621 kb on - strandat 192.621 kb on - strandat 192.621 kb on - strandat 192.621 kb on - strandat 192.624 kb on + strandat 192.624 kb on + strandat 192.624 kb on + strandat 192.624 kb on + strandat 192.624 kb on + strandat 192.624 kb on + strandat 192.624 kb on + strandat 192.625 kb on - strandat 192.625 kb on - strandat 192.625 kb on - strandat 192.625 kb on - strandat 192.628 kb on + strandat 192.629 kb on - strandat 192.768 kb on + strand, within GFF5572at 192.768 kb on + strand, within GFF5572at 192.768 kb on + strand, within GFF5572at 192.768 kb on + strand, within GFF5572at 192.768 kb on + strand, within GFF5572at 192.768 kb on + strand, within GFF5572at 192.769 kb on - strand, within GFF5572at 192.769 kb on - strand, within GFF5572at 192.769 kb on - strand, within GFF5572at 192.769 kb on - strand, within GFF5572at 192.769 kb on - strand, within GFF5572at 192.805 kb on + strand, within GFF5572at 192.849 kb on + strand, within GFF5572at 192.849 kb on + strand, within GFF5572at 192.849 kb on + strand, within GFF5572at 192.849 kb on + strand, within GFF5572at 192.849 kb on + strand, within GFF5572at 192.849 kb on + strand, within GFF5572at 192.850 kb on - strand, within GFF5572at 192.858 kb on + strand, within GFF5572at 192.858 kb on + strand, within GFF5572at 192.858 kb on + strand, within GFF5572at 192.858 kb on + strand, within GFF5572at 192.858 kb on + strand, within GFF5572at 192.859 kb on - strand, within GFF5572at 192.859 kb on - strand, within GFF5572at 192.859 kb on - strand, within GFF5572at 192.859 kb on - strand, within GFF5572at 192.859 kb on - strand, within GFF5572at 192.859 kb on - strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.919 kb on + strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.920 kb on - strand, within GFF5572at 192.931 kb on + strand, within GFF5572at 192.932 kb on - strand, within GFF5572at 193.073 kb on + strand, within GFF5572at 193.073 kb on + strand, within GFF5572at 193.074 kb on - strand, within GFF5572at 193.104 kb on + strand, within GFF5572at 193.104 kb on + strand, within GFF5572at 193.161 kb on + strand, within GFF5572at 193.256 kb on + strandat 193.256 kb on + strandat 193.256 kb on + strandat 193.257 kb on - strandat 193.321 kb on + strandat 193.321 kb on + strandat 193.373 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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191,983 + +0.6
192,417 + +0.1
192,417 + -0.4
192,518 + +0.2
192,620 + -1.2
192,621 - -0.5
192,621 - -1.0
192,621 - -0.9
192,621 - -0.2
192,624 + -0.0
192,624 + -0.6
192,624 + -0.0
192,624 + -0.4
192,624 + -1.3
192,624 + +0.6
192,624 + +1.1
192,625 - +0.0
192,625 - -0.2
192,625 - -0.1
192,625 - -1.0
192,628 + +0.8
192,629 - -0.1
192,768 + GFF5572 0.20 -0.8
192,768 + GFF5572 0.20 -0.2
192,768 + GFF5572 0.20 +0.3
192,768 + GFF5572 0.20 -1.0
192,768 + GFF5572 0.20 +0.2
192,768 + GFF5572 0.20 +0.3
192,769 - GFF5572 0.20 -0.0
192,769 - GFF5572 0.20 -0.4
192,769 - GFF5572 0.20 +0.2
192,769 - GFF5572 0.20 -0.0
192,769 - GFF5572 0.20 -0.1
192,805 + GFF5572 0.26 +1.6
192,849 + GFF5572 0.32 -0.1
192,849 + GFF5572 0.32 -0.2
192,849 + GFF5572 0.32 +0.5
192,849 + GFF5572 0.32 +1.1
192,849 + GFF5572 0.32 +1.6
192,849 + GFF5572 0.32 -0.9
192,850 - GFF5572 0.33 -0.1
192,858 + GFF5572 0.34 +0.1
192,858 + GFF5572 0.34 +1.8
192,858 + GFF5572 0.34 -1.0
192,858 + GFF5572 0.34 +0.2
192,858 + GFF5572 0.34 +0.5
192,859 - GFF5572 0.34 -0.2
192,859 - GFF5572 0.34 -1.9
192,859 - GFF5572 0.34 +0.2
192,859 - GFF5572 0.34 -0.1
192,859 - GFF5572 0.34 +1.2
192,859 - GFF5572 0.34 -0.5
192,919 + GFF5572 0.43 +0.0
192,919 + GFF5572 0.43 +0.2
192,919 + GFF5572 0.43 -1.0
192,919 + GFF5572 0.43 +0.3
192,919 + GFF5572 0.43 +0.2
192,919 + GFF5572 0.43 -1.0
192,919 + GFF5572 0.43 -0.0
192,919 + GFF5572 0.43 -1.3
192,919 + GFF5572 0.43 -0.4
192,920 - GFF5572 0.43 -0.2
192,920 - GFF5572 0.43 +0.6
192,920 - GFF5572 0.43 +0.0
192,920 - GFF5572 0.43 +0.0
192,920 - GFF5572 0.43 -0.4
192,920 - GFF5572 0.43 -0.1
192,920 - GFF5572 0.43 -0.7
192,920 - GFF5572 0.43 -0.1
192,920 - GFF5572 0.43 -2.0
192,920 - GFF5572 0.43 -0.5
192,920 - GFF5572 0.43 -0.2
192,920 - GFF5572 0.43 +0.3
192,920 - GFF5572 0.43 -1.0
192,931 + GFF5572 0.45 +0.6
192,932 - GFF5572 0.45 +0.4
193,073 + GFF5572 0.67 +1.0
193,073 + GFF5572 0.67 +0.2
193,074 - GFF5572 0.67 -0.2
193,104 + GFF5572 0.72 -0.0
193,104 + GFF5572 0.72 -1.1
193,161 + GFF5572 0.80 +1.4
193,256 + -0.7
193,256 + -0.0
193,256 + +0.2
193,257 - -0.3
193,321 + +0.8
193,321 + -1.5
193,373 + +1.8

Or see this region's nucleotide sequence