Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5565 and GFF5566 are separated by 77 nucleotides GFF5566 and GFF5567 are separated by 23 nucleotides GFF5567 and GFF5568 are separated by 12 nucleotides GFF5568 and GFF5569 are separated by 132 nucleotides GFF5569 and GFF5570 are separated by 142 nucleotides
GFF5565 - Protein translocase subunit SecY, at 187,730 to 189,043
GFF5565
GFF5566 - LSU ribosomal protein L36p @ LSU ribosomal protein L36p, zinc-dependent, at 189,121 to 189,234
GFF5566
GFF5567 - SSU ribosomal protein S13p (S18e), at 189,258 to 189,623
GFF5567
GFF5568 - SSU ribosomal protein S11p (S14e), at 189,636 to 190,040
GFF5568
GFF5569 - SSU ribosomal protein S4p (S9e) @ SSU ribosomal protein S4p (S9e), zinc-independent, at 190,173 to 190,796
GFF5569
GFF5570 - DNA-directed RNA polymerase alpha subunit (EC 2.7.7.6), at 190,939 to 191,928
GFF5570
Position (kb) 189
190
191 Strain fitness (log2 ratio) -1
0
1 at 189.025 kb on + strand at 189.025 kb on + strand at 189.041 kb on + strand at 189.041 kb on + strand at 189.099 kb on + strand at 189.099 kb on + strand at 190.841 kb on + strand at 190.841 kb on + strand at 190.841 kb on + strand at 190.841 kb on + strand at 190.841 kb on + strand at 190.841 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 189,025 + -0.2 189,025 + +0.3 189,041 + -0.6 189,041 + -0.8 189,099 + -1.0 189,099 + -0.2 190,841 + -1.6 190,841 + -0.2 190,841 + -0.2 190,841 + +0.3 190,841 + +1.4 190,841 + -0.3
Or see this region's nucleotide sequence