Strain Fitness in Variovorax sp. SCN45 around GFF5565

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5562 and GFF5563 are separated by 24 nucleotidesGFF5563 and GFF5564 are separated by 11 nucleotidesGFF5564 and GFF5565 are separated by 27 nucleotidesGFF5565 and GFF5566 are separated by 77 nucleotidesGFF5566 and GFF5567 are separated by 23 nucleotidesGFF5567 and GFF5568 are separated by 12 nucleotides GFF5562 - SSU ribosomal protein S5p (S2e), at 186,534 to 187,052 GFF5562 GFF5563 - LSU ribosomal protein L30p (L7e), at 187,077 to 187,259 GFF5563 GFF5564 - LSU ribosomal protein L15p (L27Ae), at 187,271 to 187,702 GFF5564 GFF5565 - Protein translocase subunit SecY, at 187,730 to 189,043 GFF5565 GFF5566 - LSU ribosomal protein L36p @ LSU ribosomal protein L36p, zinc-dependent, at 189,121 to 189,234 GFF5566 GFF5567 - SSU ribosomal protein S13p (S18e), at 189,258 to 189,623 GFF5567 GFF5568 - SSU ribosomal protein S11p (S14e), at 189,636 to 190,040 GFF5568 Position (kb) 187 188 189 190Strain fitness (log2 ratio) -1 0 1at 189.025 kb on + strandat 189.025 kb on + strandat 189.041 kb on + strandat 189.041 kb on + strandat 189.099 kb on + strandat 189.099 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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189,025 + -0.2
189,025 + +0.3
189,041 + -0.6
189,041 + -0.8
189,099 + -1.0
189,099 + -0.2

Or see this region's nucleotide sequence