Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5511 and GFF5512 are separated by 36 nucleotides GFF5512 and GFF5513 overlap by 4 nucleotides GFF5513 and GFF5514 overlap by 4 nucleotides
GFF5511 - FIG000875: Thioredoxin domain-containing protein EC-YbbN, at 141,849 to 142,769
GFF5511
GFF5512 - N5-carboxyaminoimidazole ribonucleotide mutase (EC 5.4.99.18), at 142,806 to 143,360
GFF5512
GFF5513 - N5-carboxyaminoimidazole ribonucleotide synthase (EC 6.3.4.18), at 143,357 to 144,547
GFF5513
GFF5514 - Threonylcarbamoyl-AMP synthase (EC 2.7.7.87) / SUA5 domain with internal deletion, at 144,544 to 145,545
GFF5514
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 142.627 kb on + strand, within GFF5511 at 142.627 kb on + strand, within GFF5511 at 142.628 kb on - strand, within GFF5511 at 142.628 kb on - strand, within GFF5511 at 142.793 kb on + strand at 142.793 kb on + strand at 142.794 kb on - strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.831 kb on + strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.832 kb on - strand at 142.956 kb on + strand, within GFF5512 at 142.957 kb on - strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.109 kb on + strand, within GFF5512 at 143.110 kb on - strand, within GFF5512 at 143.110 kb on - strand, within GFF5512 at 143.459 kb on + strand at 143.459 kb on + strand at 143.534 kb on + strand, within GFF5513 at 143.534 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.107 kb on + strand, within GFF5513 at 144.200 kb on + strand, within GFF5513 at 144.200 kb on + strand, within GFF5513 at 144.404 kb on + strand, within GFF5513 at 144.404 kb on + strand, within GFF5513 at 144.404 kb on + strand, within GFF5513 at 144.405 kb on - strand, within GFF5513
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 142,627 + GFF5511 0.84 +0.8 142,627 + GFF5511 0.84 +0.4 142,628 - GFF5511 0.85 +0.5 142,628 - GFF5511 0.85 +0.2 142,793 + +0.7 142,793 + +0.4 142,794 - +0.1 142,831 + -0.2 142,831 + +0.8 142,831 + -0.2 142,831 + +0.2 142,831 + -1.2 142,832 - +1.8 142,832 - +1.2 142,832 - +0.8 142,832 - +0.2 142,832 - -1.3 142,832 - +0.8 142,832 - -0.2 142,956 + GFF5512 0.27 +0.2 142,957 - GFF5512 0.27 -2.0 143,109 + GFF5512 0.55 -0.8 143,109 + GFF5512 0.55 -1.2 143,109 + GFF5512 0.55 -0.8 143,109 + GFF5512 0.55 -1.3 143,109 + GFF5512 0.55 -2.3 143,109 + GFF5512 0.55 -2.6 143,109 + GFF5512 0.55 -2.0 143,109 + GFF5512 0.55 -0.5 143,110 - GFF5512 0.55 +0.2 143,110 - GFF5512 0.55 +0.2 143,459 + -1.5 143,459 + -0.5 143,534 + GFF5513 0.15 -1.5 143,534 + GFF5513 0.15 +0.2 144,107 + GFF5513 0.63 -0.2 144,107 + GFF5513 0.63 -1.2 144,107 + GFF5513 0.63 -1.6 144,107 + GFF5513 0.63 -1.9 144,107 + GFF5513 0.63 -0.2 144,200 + GFF5513 0.71 -1.5 144,200 + GFF5513 0.71 -0.5 144,404 + GFF5513 0.88 -1.0 144,404 + GFF5513 0.88 -1.6 144,404 + GFF5513 0.88 -0.2 144,405 - GFF5513 0.88 -1.5
Or see this region's nucleotide sequence