Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF548 and GFF549 are separated by 15 nucleotides GFF549 and GFF550 are separated by 17 nucleotides GFF550 and GFF551 are separated by 189 nucleotides GFF551 and GFF552 are separated by 86 nucleotides
GFF548 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 590,130 to 591,110
GFF548
GFF549 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 591,126 to 591,776
GFF549
GFF550 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 591,794 to 593,170
GFF550
GFF551 - Uncharacterized protein Tbd_1903, at 593,360 to 593,947
GFF551
GFF552 - Peptidyl-prolyl cis-trans isomerase PpiA precursor (EC 5.2.1.8), at 594,034 to 594,627
GFF552
Position (kb) 591
592
593
594 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 591.166 kb on + strand at 591.166 kb on + strand at 591.166 kb on + strand at 591.166 kb on + strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.167 kb on - strand at 591.188 kb on - strand at 591.460 kb on + strand, within GFF549 at 591.461 kb on - strand, within GFF549 at 591.461 kb on - strand, within GFF549 at 591.461 kb on - strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.519 kb on + strand, within GFF549 at 591.520 kb on - strand, within GFF549 at 591.520 kb on - strand, within GFF549 at 591.520 kb on - strand, within GFF549 at 591.520 kb on - strand, within GFF549 at 591.730 kb on + strand at 591.730 kb on + strand at 591.731 kb on - strand at 591.731 kb on - strand at 591.731 kb on - strand at 591.740 kb on + strand at 591.740 kb on + strand at 591.740 kb on + strand at 591.741 kb on - strand at 591.741 kb on - strand at 591.741 kb on - strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.742 kb on + strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.743 kb on - strand at 591.750 kb on + strand at 591.750 kb on + strand at 591.750 kb on + strand at 591.750 kb on + strand at 591.751 kb on - strand at 591.751 kb on - strand at 591.751 kb on - strand at 591.751 kb on - strand at 591.751 kb on - strand at 591.751 kb on - strand at 591.788 kb on + strand at 591.789 kb on - strand at 591.789 kb on - strand at 593.279 kb on - strand at 593.279 kb on - strand at 593.291 kb on - strand at 593.291 kb on - strand at 593.291 kb on - strand at 593.291 kb on - strand at 593.291 kb on - strand at 593.291 kb on - strand at 593.291 kb on - strand at 593.291 kb on - strand at 593.294 kb on - strand at 593.295 kb on + strand at 593.295 kb on + strand at 593.295 kb on + strand at 593.295 kb on + strand at 593.296 kb on - strand at 593.296 kb on - strand at 593.296 kb on - strand at 593.296 kb on - strand at 593.296 kb on - strand at 593.296 kb on - strand at 593.296 kb on - strand at 593.296 kb on - strand at 593.313 kb on + strand at 593.313 kb on + strand at 593.313 kb on + strand at 593.313 kb on + strand at 593.313 kb on + strand at 593.313 kb on + strand at 593.314 kb on - strand at 593.314 kb on - strand at 593.400 kb on + strand at 593.400 kb on + strand at 593.400 kb on + strand at 593.400 kb on + strand at 593.400 kb on + strand at 593.400 kb on + strand at 593.400 kb on + strand at 593.400 kb on + strand at 593.400 kb on + strand at 593.401 kb on - strand at 593.401 kb on - strand at 593.401 kb on - strand at 593.441 kb on + strand, within GFF551 at 593.441 kb on + strand, within GFF551 at 593.441 kb on + strand, within GFF551 at 593.441 kb on + strand, within GFF551 at 593.441 kb on + strand, within GFF551 at 593.442 kb on - strand, within GFF551 at 593.442 kb on - strand, within GFF551 at 593.442 kb on - strand, within GFF551 at 593.455 kb on + strand, within GFF551 at 593.455 kb on + strand, within GFF551 at 593.455 kb on + strand, within GFF551 at 593.455 kb on + strand, within GFF551 at 593.456 kb on - strand, within GFF551 at 593.456 kb on - strand, within GFF551 at 593.456 kb on - strand, within GFF551 at 593.456 kb on - strand, within GFF551 at 593.456 kb on - strand, within GFF551 at 593.456 kb on - strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.507 kb on + strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.508 kb on - strand, within GFF551 at 593.642 kb on + strand, within GFF551 at 593.642 kb on + strand, within GFF551 at 593.642 kb on + strand, within GFF551 at 593.642 kb on + strand, within GFF551 at 593.642 kb on + strand, within GFF551 at 593.642 kb on + strand, within GFF551 at 593.642 kb on + strand, within GFF551 at 593.643 kb on - strand, within GFF551 at 593.643 kb on - strand, within GFF551 at 593.643 kb on - strand, within GFF551 at 593.643 kb on - strand, within GFF551 at 593.663 kb on + strand, within GFF551 at 593.663 kb on + strand, within GFF551 at 593.663 kb on + strand, within GFF551 at 593.664 kb on - strand, within GFF551 at 593.664 kb on - strand, within GFF551 at 593.664 kb on - strand, within GFF551 at 593.664 kb on - strand, within GFF551 at 593.664 kb on - strand, within GFF551 at 593.664 kb on - strand, within GFF551 at 593.664 kb on - strand, within GFF551 at 593.732 kb on + strand, within GFF551 at 593.732 kb on + strand, within GFF551 at 593.732 kb on + strand, within GFF551 at 593.732 kb on + strand, within GFF551 at 593.732 kb on + strand, within GFF551 at 593.732 kb on + strand, within GFF551 at 593.732 kb on + strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.733 kb on - strand, within GFF551 at 593.765 kb on + strand, within GFF551 at 593.765 kb on + strand, within GFF551 at 593.766 kb on - strand, within GFF551 at 593.766 kb on - strand, within GFF551 at 593.766 kb on - strand, within GFF551 at 593.766 kb on - strand, within GFF551 at 593.766 kb on - strand, within GFF551 at 593.766 kb on - strand, within GFF551 at 593.766 kb on - strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.789 kb on + strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.790 kb on - strand, within GFF551 at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.945 kb on + strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 593.946 kb on - strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.015 kb on + strand at 594.016 kb on - strand at 594.016 kb on - strand at 594.016 kb on - strand at 594.016 kb on - strand at 594.016 kb on - strand at 594.016 kb on - strand at 594.016 kb on - strand at 594.016 kb on - strand at 594.021 kb on + strand at 594.021 kb on + strand at 594.021 kb on + strand at 594.022 kb on - strand at 594.022 kb on - strand at 594.022 kb on - strand at 594.022 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 591,166 + -2.0 591,166 + -1.4 591,166 + +0.5 591,166 + -0.2 591,167 - +1.9 591,167 - +3.5 591,167 - -0.7 591,167 - +0.5 591,167 - -0.3 591,167 - -2.0 591,167 - +0.8 591,167 - -1.1 591,167 - -0.3 591,167 - -0.7 591,167 - -0.5 591,188 - -0.6 591,460 + GFF549 0.51 -1.5 591,461 - GFF549 0.51 +0.5 591,461 - GFF549 0.51 -1.4 591,461 - GFF549 0.51 -1.1 591,519 + GFF549 0.60 +0.8 591,519 + GFF549 0.60 +0.2 591,519 + GFF549 0.60 +0.6 591,519 + GFF549 0.60 -0.0 591,519 + GFF549 0.60 -0.4 591,519 + GFF549 0.60 -0.1 591,519 + GFF549 0.60 +0.3 591,519 + GFF549 0.60 +0.2 591,519 + GFF549 0.60 -0.3 591,520 - GFF549 0.61 +0.4 591,520 - GFF549 0.61 -0.8 591,520 - GFF549 0.61 -2.7 591,520 - GFF549 0.61 +0.9 591,730 + +0.1 591,730 + +0.4 591,731 - +0.2 591,731 - -0.0 591,731 - -0.8 591,740 + +0.9 591,740 + -0.6 591,740 + +0.3 591,741 - -0.4 591,741 - +0.1 591,741 - +0.6 591,742 + -0.4 591,742 + +0.2 591,742 + +0.2 591,742 + -0.1 591,742 + +1.8 591,742 + +0.1 591,742 + -0.8 591,742 + +1.3 591,742 + -0.1 591,742 + +0.4 591,742 + +1.0 591,742 + +0.5 591,742 + +0.1 591,742 + +0.1 591,742 + +0.2 591,742 + -0.9 591,742 + +1.2 591,742 + +0.1 591,743 - +0.5 591,743 - +2.8 591,743 - +0.2 591,743 - +0.1 591,743 - -1.6 591,743 - +0.4 591,743 - -2.0 591,743 - -0.6 591,743 - +0.6 591,743 - +0.4 591,743 - +0.9 591,743 - +1.2 591,743 - +1.2 591,743 - -1.3 591,743 - -0.0 591,750 + +0.2 591,750 + +0.2 591,750 + -0.2 591,750 + +0.5 591,751 - +0.6 591,751 - -0.5 591,751 - +0.8 591,751 - -0.1 591,751 - -1.1 591,751 - -0.1 591,788 + -0.2 591,789 - +0.2 591,789 - -0.0 593,279 - -0.2 593,279 - +0.1 593,291 - -0.5 593,291 - -0.5 593,291 - +1.4 593,291 - -0.4 593,291 - -0.8 593,291 - +0.7 593,291 - -1.0 593,291 - -1.2 593,294 - +0.6 593,295 + -0.0 593,295 + +0.6 593,295 + -0.7 593,295 + +0.3 593,296 - -1.0 593,296 - -0.5 593,296 - +0.5 593,296 - -0.2 593,296 - +0.9 593,296 - -1.0 593,296 - +0.2 593,296 - -0.3 593,313 + +0.3 593,313 + -0.2 593,313 + +0.3 593,313 + -0.4 593,313 + +0.6 593,313 + -1.1 593,314 - +1.0 593,314 - +0.3 593,400 + +0.8 593,400 + -0.7 593,400 + +1.2 593,400 + -1.1 593,400 + -0.8 593,400 + +0.0 593,400 + +0.7 593,400 + -2.3 593,400 + -0.7 593,401 - +0.3 593,401 - -2.1 593,401 - -0.4 593,441 + GFF551 0.14 -1.7 593,441 + GFF551 0.14 -0.6 593,441 + GFF551 0.14 -0.1 593,441 + GFF551 0.14 -0.4 593,441 + GFF551 0.14 +0.2 593,442 - GFF551 0.14 -0.5 593,442 - GFF551 0.14 -0.7 593,442 - GFF551 0.14 -0.6 593,455 + GFF551 0.16 -1.6 593,455 + GFF551 0.16 -0.6 593,455 + GFF551 0.16 -0.5 593,455 + GFF551 0.16 -0.5 593,456 - GFF551 0.16 -0.5 593,456 - GFF551 0.16 +1.2 593,456 - GFF551 0.16 -0.5 593,456 - GFF551 0.16 -0.2 593,456 - GFF551 0.16 -0.6 593,456 - GFF551 0.16 +0.5 593,507 + GFF551 0.25 -0.5 593,507 + GFF551 0.25 +0.1 593,507 + GFF551 0.25 +0.2 593,507 + GFF551 0.25 -0.4 593,507 + GFF551 0.25 -0.3 593,507 + GFF551 0.25 +0.2 593,507 + GFF551 0.25 +0.7 593,507 + GFF551 0.25 +0.3 593,507 + GFF551 0.25 -0.9 593,507 + GFF551 0.25 -1.1 593,507 + GFF551 0.25 -0.4 593,507 + GFF551 0.25 -0.5 593,507 + GFF551 0.25 -0.0 593,507 + GFF551 0.25 -1.5 593,507 + GFF551 0.25 -0.9 593,508 - GFF551 0.25 +3.2 593,508 - GFF551 0.25 -0.7 593,508 - GFF551 0.25 -0.9 593,508 - GFF551 0.25 -0.2 593,508 - GFF551 0.25 -1.1 593,508 - GFF551 0.25 +1.0 593,508 - GFF551 0.25 -0.0 593,508 - GFF551 0.25 -0.5 593,508 - GFF551 0.25 -0.0 593,508 - GFF551 0.25 +0.1 593,508 - GFF551 0.25 -0.6 593,508 - GFF551 0.25 +0.7 593,508 - GFF551 0.25 -0.9 593,508 - GFF551 0.25 -0.2 593,508 - GFF551 0.25 -0.0 593,508 - GFF551 0.25 -0.2 593,642 + GFF551 0.48 +0.4 593,642 + GFF551 0.48 +0.3 593,642 + GFF551 0.48 +0.4 593,642 + GFF551 0.48 -0.1 593,642 + GFF551 0.48 +0.1 593,642 + GFF551 0.48 +1.3 593,642 + GFF551 0.48 -0.8 593,643 - GFF551 0.48 -0.2 593,643 - GFF551 0.48 +0.8 593,643 - GFF551 0.48 -0.2 593,643 - GFF551 0.48 -0.1 593,663 + GFF551 0.52 -0.2 593,663 + GFF551 0.52 +0.8 593,663 + GFF551 0.52 +0.2 593,664 - GFF551 0.52 -0.3 593,664 - GFF551 0.52 +0.6 593,664 - GFF551 0.52 +0.0 593,664 - GFF551 0.52 -0.9 593,664 - GFF551 0.52 -0.3 593,664 - GFF551 0.52 -0.4 593,664 - GFF551 0.52 +0.5 593,732 + GFF551 0.63 -1.6 593,732 + GFF551 0.63 -0.0 593,732 + GFF551 0.63 +0.3 593,732 + GFF551 0.63 -0.2 593,732 + GFF551 0.63 -0.2 593,732 + GFF551 0.63 -0.3 593,732 + GFF551 0.63 +0.9 593,733 - GFF551 0.63 +0.3 593,733 - GFF551 0.63 -0.1 593,733 - GFF551 0.63 -0.4 593,733 - GFF551 0.63 -0.7 593,733 - GFF551 0.63 +0.0 593,733 - GFF551 0.63 -1.4 593,733 - GFF551 0.63 +0.2 593,733 - GFF551 0.63 -1.4 593,733 - GFF551 0.63 -1.3 593,733 - GFF551 0.63 +0.6 593,733 - GFF551 0.63 -0.4 593,733 - GFF551 0.63 +0.9 593,733 - GFF551 0.63 +0.1 593,733 - GFF551 0.63 +1.8 593,765 + GFF551 0.69 -0.8 593,765 + GFF551 0.69 -0.6 593,766 - GFF551 0.69 -0.5 593,766 - GFF551 0.69 -1.1 593,766 - GFF551 0.69 +0.9 593,766 - GFF551 0.69 -2.0 593,766 - GFF551 0.69 -0.2 593,766 - GFF551 0.69 +0.7 593,766 - GFF551 0.69 -0.9 593,789 + GFF551 0.73 -0.6 593,789 + GFF551 0.73 -0.0 593,789 + GFF551 0.73 +1.5 593,789 + GFF551 0.73 -0.9 593,789 + GFF551 0.73 +0.3 593,789 + GFF551 0.73 +0.3 593,789 + GFF551 0.73 +0.9 593,789 + GFF551 0.73 -1.0 593,789 + GFF551 0.73 -0.6 593,789 + GFF551 0.73 -0.8 593,790 - GFF551 0.73 +1.2 593,790 - GFF551 0.73 +0.3 593,790 - GFF551 0.73 -0.2 593,790 - GFF551 0.73 +0.9 593,790 - GFF551 0.73 -0.3 593,790 - GFF551 0.73 -1.6 593,790 - GFF551 0.73 -0.8 593,790 - GFF551 0.73 +0.6 593,790 - GFF551 0.73 +2.8 593,790 - GFF551 0.73 +0.8 593,945 + -0.6 593,945 + +0.1 593,945 + +0.1 593,945 + -1.0 593,945 + -0.3 593,945 + -0.3 593,945 + +0.1 593,945 + +0.5 593,945 + +1.4 593,945 + +0.4 593,945 + -0.6 593,945 + -0.7 593,945 + -0.7 593,946 - -0.6 593,946 - +0.3 593,946 - -0.2 593,946 - -0.7 593,946 - +0.2 593,946 - +1.3 593,946 - +0.1 593,946 - -1.6 593,946 - +0.9 593,946 - +1.8 593,946 - +0.3 593,946 - -0.4 593,946 - +1.0 594,015 + -0.7 594,015 + -0.2 594,015 + +0.4 594,015 + -0.5 594,015 + +0.9 594,015 + -1.3 594,015 + +0.6 594,015 + -0.3 594,015 + +0.2 594,015 + +1.5 594,016 - +0.3 594,016 - -0.3 594,016 - +0.6 594,016 - +0.9 594,016 - -0.4 594,016 - +0.2 594,016 - -1.4 594,016 - -0.2 594,021 + -1.4 594,021 + -0.3 594,021 + -0.2 594,022 - -0.5 594,022 - +0.5 594,022 - -1.6 594,022 - -0.4
Or see this region's nucleotide sequence