Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF545 and GFF546 are separated by 290 nucleotides GFF546 and GFF547 are separated by 54 nucleotides
GFF545 - hypothetical protein, at 586,158 to 587,525
GFF545
GFF546 - Aspartokinase (EC 2.7.2.4), at 587,816 to 589,084
GFF546
GFF547 - tRNA(Ile)-lysidine synthetase (EC 6.3.4.19), at 589,139 to 590,116
GFF547
Position (kb) 587
588
589
590 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 586.818 kb on + strand, within GFF545 at 586.818 kb on + strand, within GFF545 at 586.818 kb on + strand, within GFF545 at 586.818 kb on + strand, within GFF545 at 586.819 kb on - strand, within GFF545 at 586.819 kb on - strand, within GFF545 at 586.819 kb on - strand, within GFF545 at 586.885 kb on + strand, within GFF545 at 586.885 kb on + strand, within GFF545 at 586.886 kb on - strand, within GFF545 at 586.886 kb on - strand, within GFF545 at 586.895 kb on + strand, within GFF545 at 586.896 kb on - strand, within GFF545 at 586.896 kb on - strand, within GFF545 at 586.980 kb on + strand, within GFF545 at 586.980 kb on + strand, within GFF545 at 586.980 kb on + strand, within GFF545 at 586.980 kb on + strand, within GFF545 at 586.980 kb on + strand, within GFF545 at 586.980 kb on + strand, within GFF545 at 586.981 kb on - strand, within GFF545 at 586.981 kb on - strand, within GFF545 at 586.981 kb on - strand, within GFF545 at 586.981 kb on - strand, within GFF545 at 586.981 kb on - strand, within GFF545 at 587.067 kb on + strand, within GFF545 at 587.067 kb on + strand, within GFF545 at 587.067 kb on + strand, within GFF545 at 587.068 kb on - strand, within GFF545 at 587.100 kb on + strand, within GFF545 at 587.109 kb on + strand, within GFF545 at 587.110 kb on - strand, within GFF545 at 587.115 kb on - strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.301 kb on + strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.302 kb on - strand, within GFF545 at 587.305 kb on + strand, within GFF545 at 587.305 kb on + strand, within GFF545 at 587.305 kb on + strand, within GFF545 at 587.305 kb on + strand, within GFF545 at 587.306 kb on - strand, within GFF545 at 587.306 kb on - strand, within GFF545 at 587.306 kb on - strand, within GFF545 at 587.306 kb on - strand, within GFF545 at 587.306 kb on - strand, within GFF545 at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.466 kb on + strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.467 kb on - strand at 587.671 kb on + strand at 587.671 kb on + strand at 587.671 kb on + strand at 587.671 kb on + strand at 587.671 kb on + strand at 587.671 kb on + strand at 587.683 kb on + strand at 587.683 kb on + strand at 587.683 kb on + strand at 587.683 kb on + strand at 587.683 kb on + strand at 587.683 kb on + strand at 587.684 kb on - strand at 587.684 kb on - strand at 587.684 kb on - strand at 587.684 kb on - strand at 587.806 kb on - strand at 587.814 kb on - strand at 587.814 kb on - strand at 587.814 kb on - strand at 587.814 kb on - strand at 587.814 kb on - strand at 587.814 kb on - strand at 587.818 kb on - strand at 587.818 kb on - strand at 587.818 kb on - strand at 587.818 kb on - strand at 587.818 kb on - strand at 587.849 kb on - strand at 587.849 kb on - strand at 587.849 kb on - strand at 587.849 kb on - strand at 587.849 kb on - strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.173 kb on + strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand at 589.174 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 586,818 + GFF545 0.48 -0.1 586,818 + GFF545 0.48 -0.1 586,818 + GFF545 0.48 -0.8 586,818 + GFF545 0.48 +3.2 586,819 - GFF545 0.48 +0.4 586,819 - GFF545 0.48 -1.1 586,819 - GFF545 0.48 -0.6 586,885 + GFF545 0.53 -0.1 586,885 + GFF545 0.53 +0.6 586,886 - GFF545 0.53 +0.0 586,886 - GFF545 0.53 -2.0 586,895 + GFF545 0.54 -1.6 586,896 - GFF545 0.54 +0.3 586,896 - GFF545 0.54 +1.8 586,980 + GFF545 0.60 -1.4 586,980 + GFF545 0.60 -1.0 586,980 + GFF545 0.60 +0.0 586,980 + GFF545 0.60 -1.1 586,980 + GFF545 0.60 -0.6 586,980 + GFF545 0.60 -1.4 586,981 - GFF545 0.60 -3.1 586,981 - GFF545 0.60 -0.7 586,981 - GFF545 0.60 +0.5 586,981 - GFF545 0.60 +0.9 586,981 - GFF545 0.60 +0.6 587,067 + GFF545 0.66 +1.2 587,067 + GFF545 0.66 -0.1 587,067 + GFF545 0.66 +0.4 587,068 - GFF545 0.67 -0.3 587,100 + GFF545 0.69 +0.0 587,109 + GFF545 0.70 +1.1 587,110 - GFF545 0.70 -1.0 587,115 - GFF545 0.70 +0.1 587,301 + GFF545 0.84 +0.4 587,301 + GFF545 0.84 -0.5 587,301 + GFF545 0.84 -0.3 587,301 + GFF545 0.84 -0.1 587,301 + GFF545 0.84 -0.8 587,301 + GFF545 0.84 -0.5 587,301 + GFF545 0.84 +1.2 587,301 + GFF545 0.84 +0.0 587,301 + GFF545 0.84 +0.9 587,301 + GFF545 0.84 -0.2 587,301 + GFF545 0.84 -0.1 587,301 + GFF545 0.84 -0.4 587,301 + GFF545 0.84 -0.6 587,301 + GFF545 0.84 -0.1 587,301 + GFF545 0.84 -0.7 587,301 + GFF545 0.84 +0.8 587,301 + GFF545 0.84 -0.2 587,301 + GFF545 0.84 +0.1 587,301 + GFF545 0.84 -0.2 587,301 + GFF545 0.84 -0.9 587,301 + GFF545 0.84 +0.0 587,301 + GFF545 0.84 +0.4 587,301 + GFF545 0.84 -1.1 587,302 - GFF545 0.84 -0.2 587,302 - GFF545 0.84 -0.7 587,302 - GFF545 0.84 -1.1 587,302 - GFF545 0.84 -0.3 587,302 - GFF545 0.84 -0.3 587,302 - GFF545 0.84 -0.8 587,302 - GFF545 0.84 +0.5 587,302 - GFF545 0.84 +0.9 587,302 - GFF545 0.84 -0.7 587,302 - GFF545 0.84 -0.2 587,302 - GFF545 0.84 -0.5 587,302 - GFF545 0.84 +0.3 587,302 - GFF545 0.84 -1.0 587,302 - GFF545 0.84 -0.2 587,302 - GFF545 0.84 +0.9 587,302 - GFF545 0.84 +1.2 587,302 - GFF545 0.84 -0.0 587,302 - GFF545 0.84 -0.5 587,302 - GFF545 0.84 +0.5 587,302 - GFF545 0.84 -0.1 587,302 - GFF545 0.84 -0.6 587,302 - GFF545 0.84 -0.4 587,302 - GFF545 0.84 +0.1 587,302 - GFF545 0.84 +0.6 587,302 - GFF545 0.84 -0.8 587,302 - GFF545 0.84 -0.6 587,302 - GFF545 0.84 -1.8 587,302 - GFF545 0.84 -1.0 587,302 - GFF545 0.84 -0.7 587,302 - GFF545 0.84 -0.5 587,302 - GFF545 0.84 +0.2 587,305 + GFF545 0.84 -0.7 587,305 + GFF545 0.84 -0.6 587,305 + GFF545 0.84 -0.1 587,305 + GFF545 0.84 +0.4 587,306 - GFF545 0.84 -1.4 587,306 - GFF545 0.84 +0.0 587,306 - GFF545 0.84 -1.8 587,306 - GFF545 0.84 +0.5 587,306 - GFF545 0.84 +1.3 587,466 + -0.5 587,466 + -0.5 587,466 + -0.1 587,466 + -0.5 587,466 + -0.9 587,466 + -3.1 587,466 + +0.2 587,466 + -0.6 587,466 + -0.0 587,466 + -0.6 587,466 + -0.5 587,466 + +0.4 587,466 + -1.2 587,466 + -1.4 587,467 - +0.1 587,467 - -0.3 587,467 - -0.4 587,467 - -1.3 587,467 - -0.1 587,467 - +0.2 587,467 - -0.8 587,467 - +0.6 587,467 - -1.4 587,467 - +0.9 587,467 - -0.9 587,467 - -0.4 587,467 - -1.0 587,467 - +0.9 587,467 - -0.1 587,467 - -0.7 587,467 - +1.7 587,467 - -0.5 587,467 - +1.4 587,467 - -0.2 587,467 - -1.0 587,467 - -0.1 587,467 - -0.9 587,467 - -0.7 587,467 - -0.1 587,467 - -0.6 587,467 - -0.3 587,467 - -0.3 587,671 + +0.2 587,671 + -0.6 587,671 + -0.4 587,671 + -0.0 587,671 + +0.3 587,671 + +0.8 587,683 + +0.4 587,683 + -1.1 587,683 + -0.2 587,683 + +0.0 587,683 + +0.4 587,683 + -1.0 587,684 - -0.7 587,684 - -0.3 587,684 - +0.2 587,684 - -0.2 587,806 - -0.4 587,814 - -1.3 587,814 - -0.2 587,814 - +1.2 587,814 - -0.3 587,814 - -0.6 587,814 - -1.4 587,818 - -0.3 587,818 - -1.3 587,818 - +0.3 587,818 - +0.9 587,818 - -0.1 587,849 - -3.6 587,849 - -2.2 587,849 - -2.1 587,849 - -1.4 587,849 - -2.5 589,173 + -0.5 589,173 + -0.0 589,173 + +0.1 589,173 + +0.6 589,173 + +0.0 589,173 + -2.3 589,173 + +0.2 589,173 + -1.1 589,173 + +0.9 589,173 + -0.2 589,173 + -0.5 589,173 + +1.1 589,173 + -0.9 589,173 + +0.5 589,174 - -0.7 589,174 - -1.6 589,174 - -0.9 589,174 - +0.7 589,174 - +0.8 589,174 - -0.2 589,174 - -0.0 589,174 - +0.1 589,174 - +0.6 589,174 - +0.9 589,174 - -1.1 589,174 - -0.1
Or see this region's nucleotide sequence