Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF53 and GFF54 are separated by 26 nucleotides GFF54 and GFF55 overlap by 4 nucleotides GFF55 and GFF56 overlap by 8 nucleotides
GFF53 - Polyketide synthase modules and related proteins, at 64,080 to 69,116
GFF53
GFF54 - 4'-phosphopantetheinyl transferase (EC 2.7.8.-), at 69,143 to 69,856
GFF54
GFF55 - hypothetical protein, at 69,853 to 70,671
GFF55
GFF56 - hypothetical protein, at 70,664 to 71,593
GFF56
Position (kb)
69
70 Strain fitness (log2 ratio)
-2
-1
0
1 at 68.286 kb on + strand, within GFF53 at 68.287 kb on - strand, within GFF53 at 68.287 kb on - strand, within GFF53 at 68.319 kb on + strand, within GFF53 at 68.320 kb on - strand, within GFF53 at 68.547 kb on + strand, within GFF53 at 68.547 kb on + strand, within GFF53 at 68.548 kb on - strand, within GFF53 at 68.548 kb on - strand, within GFF53 at 68.562 kb on + strand, within GFF53 at 68.562 kb on + strand, within GFF53 at 68.562 kb on + strand, within GFF53 at 68.563 kb on - strand, within GFF53 at 68.563 kb on - strand, within GFF53 at 68.563 kb on - strand, within GFF53 at 68.599 kb on - strand, within GFF53 at 68.599 kb on - strand, within GFF53 at 68.599 kb on - strand, within GFF53 at 68.649 kb on + strand at 68.649 kb on + strand at 68.649 kb on + strand at 68.649 kb on + strand at 68.649 kb on + strand at 68.650 kb on - strand at 68.686 kb on - strand at 68.686 kb on - strand at 68.686 kb on - strand at 68.947 kb on - strand at 70.057 kb on + strand, within GFF55 at 70.058 kb on - strand, within GFF55 at 70.335 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.570 kb on + strand, within GFF55 at 70.571 kb on - strand, within GFF55
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 68,286 + GFF53 0.84 -0.2 68,287 - GFF53 0.84 -1.1 68,287 - GFF53 0.84 +0.4 68,319 + GFF53 0.84 -1.0 68,320 - GFF53 0.84 +0.5 68,547 + GFF53 0.89 +1.6 68,547 + GFF53 0.89 -1.4 68,548 - GFF53 0.89 +0.1 68,548 - GFF53 0.89 -0.4 68,562 + GFF53 0.89 +1.5 68,562 + GFF53 0.89 -0.4 68,562 + GFF53 0.89 -0.1 68,563 - GFF53 0.89 -0.0 68,563 - GFF53 0.89 -0.1 68,563 - GFF53 0.89 +0.3 68,599 - GFF53 0.90 -2.1 68,599 - GFF53 0.90 +0.5 68,599 - GFF53 0.90 -0.3 68,649 + -0.5 68,649 + +0.0 68,649 + +0.9 68,649 + -0.3 68,649 + -0.1 68,650 - -0.2 68,686 - -1.8 68,686 - -0.5 68,686 - -0.5 68,947 - -0.6 70,057 + GFF55 0.25 -0.7 70,058 - GFF55 0.25 +0.1 70,335 + GFF55 0.59 -0.6 70,570 + GFF55 0.88 -0.3 70,570 + GFF55 0.88 -0.2 70,570 + GFF55 0.88 +0.1 70,570 + GFF55 0.88 -0.8 70,570 + GFF55 0.88 -1.1 70,570 + GFF55 0.88 -1.1 70,571 - GFF55 0.88 +1.0
Or see this region's nucleotide sequence