Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5376 and GFF5377 are separated by 71 nucleotides GFF5377 and GFF5378 are separated by 1 nucleotides GFF5378 and GFF5379 are separated by 92 nucleotides GFF5379 and GFF5380 are separated by 13 nucleotides
GFF5376 - hypothetical protein, at 3,177 to 4,010
GFF5376
GFF5377 - Transcriptional regulator, AcrR family, at 4,082 to 4,717
GFF5377
GFF5378 - hypothetical protein, at 4,719 to 4,928
GFF5378
GFF5379 - Putative hydrolase, at 5,021 to 5,893
GFF5379
GFF5380 - BUG/TctC family periplasmic protein, at 5,907 to 6,869
GFF5380
Position (kb) 4
5 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 3.964 kb on + strand at 3.964 kb on + strand at 3.964 kb on + strand at 3.965 kb on - strand at 3.965 kb on - strand at 3.965 kb on - strand at 3.965 kb on - strand at 3.965 kb on - strand at 3.965 kb on - strand at 4.043 kb on - strand at 4.262 kb on + strand, within GFF5377 at 4.262 kb on + strand, within GFF5377 at 4.262 kb on + strand, within GFF5377 at 4.262 kb on + strand, within GFF5377 at 4.263 kb on - strand, within GFF5377 at 4.263 kb on - strand, within GFF5377 at 4.263 kb on - strand, within GFF5377 at 4.263 kb on - strand, within GFF5377 at 4.263 kb on - strand, within GFF5377 at 4.268 kb on + strand, within GFF5377 at 4.268 kb on + strand, within GFF5377 at 4.268 kb on + strand, within GFF5377 at 4.268 kb on + strand, within GFF5377 at 4.268 kb on + strand, within GFF5377 at 4.268 kb on + strand, within GFF5377 at 4.269 kb on - strand, within GFF5377 at 4.451 kb on + strand, within GFF5377 at 4.451 kb on + strand, within GFF5377 at 4.451 kb on + strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.452 kb on - strand, within GFF5377 at 4.488 kb on - strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.499 kb on + strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.500 kb on - strand, within GFF5377 at 4.604 kb on + strand, within GFF5377 at 4.605 kb on - strand, within GFF5377 at 4.605 kb on - strand, within GFF5377 at 4.694 kb on + strand at 4.695 kb on - strand at 4.941 kb on + strand at 4.941 kb on + strand at 4.941 kb on + strand at 4.941 kb on + strand at 4.942 kb on - strand at 4.942 kb on - strand at 4.942 kb on - strand at 4.942 kb on - strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.943 kb on + strand at 4.944 kb on - strand at 4.944 kb on - strand at 4.944 kb on - strand at 4.944 kb on - strand at 4.944 kb on - strand at 4.944 kb on - strand at 4.944 kb on - strand at 4.944 kb on - strand at 4.968 kb on + strand at 4.968 kb on + strand at 4.968 kb on + strand at 4.969 kb on - strand at 4.969 kb on - strand at 4.969 kb on - strand at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.124 kb on + strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.125 kb on - strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.208 kb on + strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.209 kb on - strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.220 kb on + strand, within GFF5379 at 5.221 kb on - strand, within GFF5379 at 5.221 kb on - strand, within GFF5379 at 5.221 kb on - strand, within GFF5379 at 5.221 kb on - strand, within GFF5379 at 5.221 kb on - strand, within GFF5379 at 5.221 kb on - strand, within GFF5379 at 5.232 kb on + strand, within GFF5379 at 5.232 kb on + strand, within GFF5379 at 5.232 kb on + strand, within GFF5379 at 5.398 kb on - strand, within GFF5379 at 5.398 kb on - strand, within GFF5379 at 5.398 kb on - strand, within GFF5379 at 5.448 kb on + strand, within GFF5379 at 5.449 kb on - strand, within GFF5379 at 5.449 kb on - strand, within GFF5379 at 5.449 kb on - strand, within GFF5379 at 5.508 kb on + strand, within GFF5379 at 5.508 kb on + strand, within GFF5379 at 5.508 kb on + strand, within GFF5379 at 5.508 kb on + strand, within GFF5379 at 5.509 kb on - strand, within GFF5379 at 5.509 kb on - strand, within GFF5379 at 5.509 kb on - strand, within GFF5379 at 5.509 kb on - strand, within GFF5379 at 5.509 kb on - strand, within GFF5379 at 5.509 kb on - strand, within GFF5379 at 5.661 kb on + strand, within GFF5379 at 5.661 kb on + strand, within GFF5379 at 5.661 kb on + strand, within GFF5379 at 5.661 kb on + strand, within GFF5379 at 5.662 kb on - strand, within GFF5379 at 5.662 kb on - strand, within GFF5379 at 5.662 kb on - strand, within GFF5379 at 5.662 kb on - strand, within GFF5379 at 5.662 kb on - strand, within GFF5379 at 5.662 kb on - strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.718 kb on + strand, within GFF5379 at 5.719 kb on - strand, within GFF5379 at 5.719 kb on - strand, within GFF5379 at 5.719 kb on - strand, within GFF5379 at 5.719 kb on - strand, within GFF5379 at 5.719 kb on - strand, within GFF5379 at 5.719 kb on - strand, within GFF5379 at 5.719 kb on - strand, within GFF5379 at 5.719 kb on - strand, within GFF5379 at 5.794 kb on - strand, within GFF5379 at 5.893 kb on + strand at 5.893 kb on + strand at 5.893 kb on + strand at 5.893 kb on + strand at 5.893 kb on + strand at 5.893 kb on + strand at 5.894 kb on - strand at 5.894 kb on - strand at 5.894 kb on - strand at 5.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 3,964 + +0.7 3,964 + -0.8 3,964 + -0.6 3,965 - -0.4 3,965 - -0.0 3,965 - -0.1 3,965 - -0.3 3,965 - +1.3 3,965 - +0.9 4,043 - -0.4 4,262 + GFF5377 0.28 +0.5 4,262 + GFF5377 0.28 +0.2 4,262 + GFF5377 0.28 -0.6 4,262 + GFF5377 0.28 -0.2 4,263 - GFF5377 0.28 -0.2 4,263 - GFF5377 0.28 +0.0 4,263 - GFF5377 0.28 -0.9 4,263 - GFF5377 0.28 +1.8 4,263 - GFF5377 0.28 -0.7 4,268 + GFF5377 0.29 -0.6 4,268 + GFF5377 0.29 -1.0 4,268 + GFF5377 0.29 -0.2 4,268 + GFF5377 0.29 +0.6 4,268 + GFF5377 0.29 +0.2 4,268 + GFF5377 0.29 +0.7 4,269 - GFF5377 0.29 +0.2 4,451 + GFF5377 0.58 -0.6 4,451 + GFF5377 0.58 -0.6 4,451 + GFF5377 0.58 -0.4 4,452 - GFF5377 0.58 -1.5 4,452 - GFF5377 0.58 -0.0 4,452 - GFF5377 0.58 +0.1 4,452 - GFF5377 0.58 +0.2 4,452 - GFF5377 0.58 -0.3 4,452 - GFF5377 0.58 -0.9 4,452 - GFF5377 0.58 -0.1 4,452 - GFF5377 0.58 +0.5 4,452 - GFF5377 0.58 +0.8 4,452 - GFF5377 0.58 -0.3 4,488 - GFF5377 0.64 +0.1 4,499 + GFF5377 0.66 -0.2 4,499 + GFF5377 0.66 +0.0 4,499 + GFF5377 0.66 +0.4 4,499 + GFF5377 0.66 +0.4 4,499 + GFF5377 0.66 +1.2 4,499 + GFF5377 0.66 +0.4 4,499 + GFF5377 0.66 -0.1 4,499 + GFF5377 0.66 -0.1 4,499 + GFF5377 0.66 -0.1 4,500 - GFF5377 0.66 +0.0 4,500 - GFF5377 0.66 +0.2 4,500 - GFF5377 0.66 -0.8 4,500 - GFF5377 0.66 -0.4 4,500 - GFF5377 0.66 -0.4 4,500 - GFF5377 0.66 +0.8 4,500 - GFF5377 0.66 -0.1 4,500 - GFF5377 0.66 -0.4 4,500 - GFF5377 0.66 -0.3 4,500 - GFF5377 0.66 +0.3 4,500 - GFF5377 0.66 +0.2 4,500 - GFF5377 0.66 +0.7 4,500 - GFF5377 0.66 -1.1 4,500 - GFF5377 0.66 -0.2 4,500 - GFF5377 0.66 -0.3 4,500 - GFF5377 0.66 -0.5 4,500 - GFF5377 0.66 +0.2 4,500 - GFF5377 0.66 +0.8 4,500 - GFF5377 0.66 +0.6 4,500 - GFF5377 0.66 -2.2 4,604 + GFF5377 0.82 -0.0 4,605 - GFF5377 0.82 +0.0 4,605 - GFF5377 0.82 -0.5 4,694 + -0.6 4,695 - +0.2 4,941 + -0.4 4,941 + -1.2 4,941 + -0.2 4,941 + -0.3 4,942 - -0.3 4,942 - -0.2 4,942 - +1.6 4,942 - +0.4 4,943 + +0.8 4,943 + +1.0 4,943 + +0.4 4,943 + -0.5 4,943 + -0.1 4,943 + -1.3 4,943 + +0.3 4,943 + +0.3 4,943 + +0.7 4,943 + +0.5 4,943 + -0.1 4,943 + -0.0 4,943 + -0.3 4,943 + -0.6 4,944 - +0.5 4,944 - +0.3 4,944 - +0.2 4,944 - -0.5 4,944 - -1.1 4,944 - +1.6 4,944 - +0.6 4,944 - +0.2 4,968 + -0.3 4,968 + +0.2 4,968 + -1.4 4,969 - -0.7 4,969 - -0.3 4,969 - +0.4 5,124 + GFF5379 0.12 -0.0 5,124 + GFF5379 0.12 -0.4 5,124 + GFF5379 0.12 -0.4 5,124 + GFF5379 0.12 +1.3 5,124 + GFF5379 0.12 -0.0 5,124 + GFF5379 0.12 -0.5 5,124 + GFF5379 0.12 +0.2 5,124 + GFF5379 0.12 -0.4 5,124 + GFF5379 0.12 +0.3 5,124 + GFF5379 0.12 -0.4 5,124 + GFF5379 0.12 -1.6 5,124 + GFF5379 0.12 +0.3 5,124 + GFF5379 0.12 -0.1 5,125 - GFF5379 0.12 -1.1 5,125 - GFF5379 0.12 -0.2 5,125 - GFF5379 0.12 -0.3 5,125 - GFF5379 0.12 -0.6 5,125 - GFF5379 0.12 -0.4 5,125 - GFF5379 0.12 +1.0 5,125 - GFF5379 0.12 +0.3 5,125 - GFF5379 0.12 -0.1 5,125 - GFF5379 0.12 +0.8 5,125 - GFF5379 0.12 +1.8 5,125 - GFF5379 0.12 +0.0 5,208 + GFF5379 0.21 +0.5 5,208 + GFF5379 0.21 -1.2 5,208 + GFF5379 0.21 -0.3 5,208 + GFF5379 0.21 +0.4 5,208 + GFF5379 0.21 +0.8 5,208 + GFF5379 0.21 -0.5 5,208 + GFF5379 0.21 -0.8 5,208 + GFF5379 0.21 +0.2 5,208 + GFF5379 0.21 -0.9 5,208 + GFF5379 0.21 -0.9 5,208 + GFF5379 0.21 -1.0 5,208 + GFF5379 0.21 -0.2 5,209 - GFF5379 0.22 -0.2 5,209 - GFF5379 0.22 +0.8 5,209 - GFF5379 0.22 -0.5 5,209 - GFF5379 0.22 +0.0 5,209 - GFF5379 0.22 +1.1 5,209 - GFF5379 0.22 +0.5 5,209 - GFF5379 0.22 -0.4 5,209 - GFF5379 0.22 +0.2 5,209 - GFF5379 0.22 -0.3 5,220 + GFF5379 0.23 +0.1 5,220 + GFF5379 0.23 -0.2 5,220 + GFF5379 0.23 +2.4 5,220 + GFF5379 0.23 -0.1 5,220 + GFF5379 0.23 +0.2 5,220 + GFF5379 0.23 -0.8 5,220 + GFF5379 0.23 -1.2 5,220 + GFF5379 0.23 +0.0 5,220 + GFF5379 0.23 -0.2 5,220 + GFF5379 0.23 +0.8 5,220 + GFF5379 0.23 -1.1 5,220 + GFF5379 0.23 -0.0 5,221 - GFF5379 0.23 +0.5 5,221 - GFF5379 0.23 -1.3 5,221 - GFF5379 0.23 -0.4 5,221 - GFF5379 0.23 +0.5 5,221 - GFF5379 0.23 -0.1 5,221 - GFF5379 0.23 -0.0 5,232 + GFF5379 0.24 +0.4 5,232 + GFF5379 0.24 -0.0 5,232 + GFF5379 0.24 +0.3 5,398 - GFF5379 0.43 +0.0 5,398 - GFF5379 0.43 -0.3 5,398 - GFF5379 0.43 +0.1 5,448 + GFF5379 0.49 -0.2 5,449 - GFF5379 0.49 +0.2 5,449 - GFF5379 0.49 +0.3 5,449 - GFF5379 0.49 -1.6 5,508 + GFF5379 0.56 +0.1 5,508 + GFF5379 0.56 -1.5 5,508 + GFF5379 0.56 -0.3 5,508 + GFF5379 0.56 -0.2 5,509 - GFF5379 0.56 -0.3 5,509 - GFF5379 0.56 -1.9 5,509 - GFF5379 0.56 -0.3 5,509 - GFF5379 0.56 +0.1 5,509 - GFF5379 0.56 +0.0 5,509 - GFF5379 0.56 -0.2 5,661 + GFF5379 0.73 +0.1 5,661 + GFF5379 0.73 -0.6 5,661 + GFF5379 0.73 +0.4 5,661 + GFF5379 0.73 +0.1 5,662 - GFF5379 0.73 -1.6 5,662 - GFF5379 0.73 -0.7 5,662 - GFF5379 0.73 +0.5 5,662 - GFF5379 0.73 +1.6 5,662 - GFF5379 0.73 -0.0 5,662 - GFF5379 0.73 -0.6 5,718 + GFF5379 0.80 -0.1 5,718 + GFF5379 0.80 +0.5 5,718 + GFF5379 0.80 +0.3 5,718 + GFF5379 0.80 +0.8 5,718 + GFF5379 0.80 +0.6 5,718 + GFF5379 0.80 +0.3 5,718 + GFF5379 0.80 +0.4 5,718 + GFF5379 0.80 -0.1 5,718 + GFF5379 0.80 -0.4 5,718 + GFF5379 0.80 +0.7 5,718 + GFF5379 0.80 +0.2 5,718 + GFF5379 0.80 -0.2 5,718 + GFF5379 0.80 +0.4 5,719 - GFF5379 0.80 -0.6 5,719 - GFF5379 0.80 -0.5 5,719 - GFF5379 0.80 -0.7 5,719 - GFF5379 0.80 +0.4 5,719 - GFF5379 0.80 -0.2 5,719 - GFF5379 0.80 -0.3 5,719 - GFF5379 0.80 -0.0 5,719 - GFF5379 0.80 +0.8 5,794 - GFF5379 0.89 +3.1 5,893 + -1.1 5,893 + -0.1 5,893 + +0.6 5,893 + -0.6 5,893 + -0.4 5,893 + -0.6 5,894 - -0.2 5,894 - +0.3 5,894 - -0.1 5,894 - +0.1
Or see this region's nucleotide sequence