Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5357 and GFF5358 are separated by 37 nucleotides GFF5358 and GFF5359 overlap by 4 nucleotides GFF5359 and GFF5360 overlap by 4 nucleotides GFF5360 and GFF5361 are separated by 2 nucleotides GFF5361 and GFF5362 are separated by 65 nucleotides
GFF5357 - Imidazole glycerol phosphate synthase cyclase subunit, at 233,558 to 234,337
GFF5357
GFF5358 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19), at 234,375 to 234,764
GFF5358
GFF5359 - Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 234,761 to 235,162
GFF5359
GFF5360 - Putative transmembrane protein, at 235,159 to 235,530
GFF5360
GFF5361 - FIG146285: Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases, at 235,533 to 235,892
GFF5361
GFF5362 - Twin-arginine translocation protein TatA, at 235,958 to 236,203
GFF5362
Position (kb) 234
235
236 Strain fitness (log2 ratio) -1
0
1
2 at 234.343 kb on - strand at 234.356 kb on + strand at 234.368 kb on + strand at 234.368 kb on + strand at 234.368 kb on + strand at 234.368 kb on + strand at 234.368 kb on + strand at 234.369 kb on - strand at 234.756 kb on + strand at 234.756 kb on + strand at 234.756 kb on + strand at 234.756 kb on + strand at 234.756 kb on + strand at 234.756 kb on + strand at 234.756 kb on + strand at 235.246 kb on + strand, within GFF5360 at 235.246 kb on + strand, within GFF5360 at 235.246 kb on + strand, within GFF5360 at 235.247 kb on - strand, within GFF5360 at 235.247 kb on - strand, within GFF5360 at 235.247 kb on - strand, within GFF5360 at 235.247 kb on - strand, within GFF5360 at 235.247 kb on - strand, within GFF5360 at 235.247 kb on - strand, within GFF5360 at 235.247 kb on - strand, within GFF5360 at 235.255 kb on + strand, within GFF5360 at 235.255 kb on + strand, within GFF5360 at 235.255 kb on + strand, within GFF5360 at 235.255 kb on + strand, within GFF5360 at 235.255 kb on + strand, within GFF5360 at 235.256 kb on - strand, within GFF5360 at 235.256 kb on - strand, within GFF5360 at 235.256 kb on - strand, within GFF5360 at 235.256 kb on - strand, within GFF5360 at 235.256 kb on - strand, within GFF5360 at 235.256 kb on - strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.312 kb on + strand, within GFF5360 at 235.313 kb on - strand, within GFF5360 at 235.313 kb on - strand, within GFF5360 at 235.313 kb on - strand, within GFF5360 at 235.313 kb on - strand, within GFF5360 at 235.313 kb on - strand, within GFF5360 at 235.313 kb on - strand, within GFF5360 at 235.313 kb on - strand, within GFF5360 at 235.313 kb on - strand, within GFF5360 at 235.345 kb on + strand, within GFF5360 at 235.346 kb on - strand, within GFF5360 at 235.346 kb on - strand, within GFF5360 at 235.474 kb on + strand, within GFF5360 at 235.474 kb on + strand, within GFF5360 at 235.475 kb on - strand, within GFF5360 at 235.475 kb on - strand, within GFF5360 at 235.475 kb on - strand, within GFF5360 at 235.600 kb on - strand, within GFF5361 at 235.900 kb on + strand at 235.918 kb on + strand at 235.918 kb on + strand at 235.918 kb on + strand at 235.918 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 234,343 - -0.3 234,356 + +0.0 234,368 + -0.2 234,368 + -0.1 234,368 + -0.5 234,368 + -0.5 234,368 + -0.4 234,369 - +0.8 234,756 + -0.4 234,756 + +0.0 234,756 + -0.4 234,756 + -0.2 234,756 + +0.6 234,756 + -0.3 234,756 + -1.3 235,246 + GFF5360 0.23 -0.6 235,246 + GFF5360 0.23 -0.7 235,246 + GFF5360 0.23 +1.3 235,247 - GFF5360 0.24 -0.1 235,247 - GFF5360 0.24 -0.3 235,247 - GFF5360 0.24 -0.4 235,247 - GFF5360 0.24 -0.6 235,247 - GFF5360 0.24 -0.0 235,247 - GFF5360 0.24 +0.8 235,247 - GFF5360 0.24 -0.2 235,255 + GFF5360 0.26 +0.2 235,255 + GFF5360 0.26 -0.0 235,255 + GFF5360 0.26 +0.3 235,255 + GFF5360 0.26 +0.0 235,255 + GFF5360 0.26 -0.5 235,256 - GFF5360 0.26 -0.3 235,256 - GFF5360 0.26 +0.1 235,256 - GFF5360 0.26 +1.6 235,256 - GFF5360 0.26 -1.1 235,256 - GFF5360 0.26 -1.1 235,256 - GFF5360 0.26 +2.3 235,312 + GFF5360 0.41 -0.3 235,312 + GFF5360 0.41 -0.4 235,312 + GFF5360 0.41 +1.3 235,312 + GFF5360 0.41 +0.8 235,312 + GFF5360 0.41 -1.2 235,312 + GFF5360 0.41 +0.4 235,312 + GFF5360 0.41 -0.2 235,312 + GFF5360 0.41 +0.4 235,312 + GFF5360 0.41 +0.2 235,312 + GFF5360 0.41 -0.2 235,312 + GFF5360 0.41 -0.2 235,313 - GFF5360 0.41 -0.3 235,313 - GFF5360 0.41 +0.2 235,313 - GFF5360 0.41 -0.6 235,313 - GFF5360 0.41 +0.1 235,313 - GFF5360 0.41 -0.7 235,313 - GFF5360 0.41 -0.4 235,313 - GFF5360 0.41 -0.7 235,313 - GFF5360 0.41 +0.5 235,345 + GFF5360 0.50 +0.8 235,346 - GFF5360 0.50 +0.6 235,346 - GFF5360 0.50 -0.1 235,474 + GFF5360 0.85 -0.2 235,474 + GFF5360 0.85 -0.6 235,475 - GFF5360 0.85 +1.6 235,475 - GFF5360 0.85 -0.5 235,475 - GFF5360 0.85 -1.0 235,600 - GFF5361 0.19 +0.1 235,900 + -0.4 235,918 + +1.3 235,918 + -0.6 235,918 + +0.0 235,918 + +0.1
Or see this region's nucleotide sequence