Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5349 and GFF5350 are separated by 19 nucleotides GFF5350 and GFF5351 are separated by 63 nucleotides GFF5351 and GFF5352 are separated by 34 nucleotides
GFF5349 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 227,033 to 227,650
GFF5349
GFF5350 - Imidazole glycerol phosphate synthase amidotransferase subunit HisH, at 227,670 to 228,326
GFF5350
GFF5351 - Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16), at 228,390 to 229,130
GFF5351
GFF5352 - Split AAA-ATPase protein PA0787, at 229,165 to 230,337
GFF5352
Position (kb) 228
229
230 Strain fitness (log2 ratio) -1
0
1
2 at 227.669 kb on + strand at 227.669 kb on + strand at 227.670 kb on - strand at 229.145 kb on + strand at 229.145 kb on + strand at 229.145 kb on + strand at 229.145 kb on + strand at 229.192 kb on + strand at 229.192 kb on + strand at 229.192 kb on + strand at 229.192 kb on + strand at 229.193 kb on - strand at 229.193 kb on - strand at 229.276 kb on + strand at 229.468 kb on - strand, within GFF5352 at 229.489 kb on + strand, within GFF5352 at 229.489 kb on + strand, within GFF5352 at 229.489 kb on + strand, within GFF5352 at 229.489 kb on + strand, within GFF5352 at 229.490 kb on - strand, within GFF5352 at 229.490 kb on - strand, within GFF5352 at 229.490 kb on - strand, within GFF5352 at 229.490 kb on - strand, within GFF5352 at 229.566 kb on + strand, within GFF5352 at 229.566 kb on + strand, within GFF5352 at 229.567 kb on - strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.768 kb on + strand, within GFF5352 at 229.769 kb on - strand, within GFF5352 at 229.769 kb on - strand, within GFF5352 at 230.047 kb on + strand, within GFF5352 at 230.047 kb on + strand, within GFF5352 at 230.047 kb on + strand, within GFF5352 at 230.047 kb on + strand, within GFF5352 at 230.048 kb on - strand, within GFF5352 at 230.048 kb on - strand, within GFF5352
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 227,669 + -0.4 227,669 + +0.0 227,670 - -1.3 229,145 + -1.4 229,145 + +0.5 229,145 + +0.7 229,145 + +0.4 229,192 + -0.3 229,192 + +0.3 229,192 + -1.2 229,192 + -0.0 229,193 - -1.7 229,193 - +0.4 229,276 + +0.5 229,468 - GFF5352 0.26 +0.0 229,489 + GFF5352 0.28 +0.0 229,489 + GFF5352 0.28 -0.6 229,489 + GFF5352 0.28 -0.2 229,489 + GFF5352 0.28 +0.1 229,490 - GFF5352 0.28 -0.2 229,490 - GFF5352 0.28 -0.4 229,490 - GFF5352 0.28 -0.3 229,490 - GFF5352 0.28 -0.9 229,566 + GFF5352 0.34 +0.1 229,566 + GFF5352 0.34 -0.6 229,567 - GFF5352 0.34 +0.1 229,768 + GFF5352 0.51 +0.2 229,768 + GFF5352 0.51 -0.3 229,768 + GFF5352 0.51 +0.5 229,768 + GFF5352 0.51 -0.3 229,768 + GFF5352 0.51 +2.6 229,768 + GFF5352 0.51 -0.4 229,768 + GFF5352 0.51 -1.6 229,768 + GFF5352 0.51 -0.3 229,768 + GFF5352 0.51 -0.9 229,768 + GFF5352 0.51 -0.7 229,769 - GFF5352 0.51 +0.0 229,769 - GFF5352 0.51 -0.5 230,047 + GFF5352 0.75 -0.2 230,047 + GFF5352 0.75 +0.8 230,047 + GFF5352 0.75 -0.1 230,047 + GFF5352 0.75 -0.2 230,048 - GFF5352 0.75 -0.7 230,048 - GFF5352 0.75 -0.3
Or see this region's nucleotide sequence