Strain Fitness in Variovorax sp. SCN45 around GFF5349

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5348 and GFF5349 overlap by 4 nucleotidesGFF5349 and GFF5350 are separated by 19 nucleotidesGFF5350 and GFF5351 are separated by 63 nucleotides GFF5348 - Histidinol-phosphate aminotransferase (EC 2.6.1.9), at 225,945 to 227,036 GFF5348 GFF5349 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 227,033 to 227,650 GFF5349 GFF5350 - Imidazole glycerol phosphate synthase amidotransferase subunit HisH, at 227,670 to 228,326 GFF5350 GFF5351 - Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16), at 228,390 to 229,130 GFF5351 Position (kb) 227 228Strain fitness (log2 ratio) -2 -1 0 1 2at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.308 kb on + strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.309 kb on - strand, within GFF5348at 226.428 kb on + strand, within GFF5348at 226.428 kb on + strand, within GFF5348at 226.429 kb on - strand, within GFF5348at 226.438 kb on - strand, within GFF5348at 226.539 kb on + strand, within GFF5348at 226.539 kb on + strand, within GFF5348at 226.539 kb on + strand, within GFF5348at 226.539 kb on + strand, within GFF5348at 226.539 kb on + strand, within GFF5348at 226.539 kb on + strand, within GFF5348at 226.539 kb on + strand, within GFF5348at 226.539 kb on + strand, within GFF5348at 226.540 kb on - strand, within GFF5348at 226.540 kb on - strand, within GFF5348at 226.540 kb on - strand, within GFF5348at 226.540 kb on - strand, within GFF5348at 226.540 kb on - strand, within GFF5348at 226.563 kb on + strand, within GFF5348at 226.563 kb on + strand, within GFF5348at 226.563 kb on + strand, within GFF5348at 226.563 kb on + strand, within GFF5348at 226.563 kb on + strand, within GFF5348at 226.563 kb on + strand, within GFF5348at 226.563 kb on + strand, within GFF5348at 226.563 kb on + strand, within GFF5348at 226.564 kb on - strand, within GFF5348at 226.564 kb on - strand, within GFF5348at 226.564 kb on - strand, within GFF5348at 226.564 kb on - strand, within GFF5348at 226.564 kb on - strand, within GFF5348at 226.564 kb on - strand, within GFF5348at 226.564 kb on - strand, within GFF5348at 226.647 kb on + strand, within GFF5348at 226.647 kb on + strand, within GFF5348at 226.648 kb on - strand, within GFF5348at 226.648 kb on - strand, within GFF5348at 226.702 kb on - strand, within GFF5348at 226.702 kb on - strand, within GFF5348at 226.702 kb on - strand, within GFF5348at 226.702 kb on - strand, within GFF5348at 226.702 kb on - strand, within GFF5348at 226.702 kb on - strand, within GFF5348at 226.940 kb on + strandat 226.940 kb on + strandat 227.032 kb on + strandat 227.032 kb on + strandat 227.032 kb on + strandat 227.032 kb on + strandat 227.032 kb on + strandat 227.032 kb on + strandat 227.032 kb on + strandat 227.032 kb on + strandat 227.033 kb on - strandat 227.033 kb on - strandat 227.669 kb on + strandat 227.669 kb on + strandat 227.670 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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226,308 + GFF5348 0.33 +0.2
226,308 + GFF5348 0.33 -0.4
226,308 + GFF5348 0.33 -1.2
226,308 + GFF5348 0.33 +0.9
226,308 + GFF5348 0.33 +1.2
226,308 + GFF5348 0.33 +0.6
226,308 + GFF5348 0.33 -0.4
226,308 + GFF5348 0.33 +0.5
226,308 + GFF5348 0.33 -0.2
226,308 + GFF5348 0.33 -0.7
226,308 + GFF5348 0.33 +0.0
226,308 + GFF5348 0.33 -1.0
226,308 + GFF5348 0.33 -0.2
226,308 + GFF5348 0.33 -0.8
226,308 + GFF5348 0.33 +0.3
226,308 + GFF5348 0.33 -0.4
226,309 - GFF5348 0.33 -0.5
226,309 - GFF5348 0.33 -0.8
226,309 - GFF5348 0.33 -0.7
226,309 - GFF5348 0.33 +0.9
226,309 - GFF5348 0.33 -0.2
226,309 - GFF5348 0.33 +0.5
226,309 - GFF5348 0.33 +0.3
226,309 - GFF5348 0.33 -0.4
226,309 - GFF5348 0.33 -0.2
226,309 - GFF5348 0.33 -0.0
226,309 - GFF5348 0.33 -0.3
226,309 - GFF5348 0.33 +0.6
226,309 - GFF5348 0.33 -0.3
226,428 + GFF5348 0.44 +1.8
226,428 + GFF5348 0.44 +1.3
226,429 - GFF5348 0.44 +0.1
226,438 - GFF5348 0.45 -1.3
226,539 + GFF5348 0.54 -0.3
226,539 + GFF5348 0.54 -1.9
226,539 + GFF5348 0.54 -0.3
226,539 + GFF5348 0.54 +0.6
226,539 + GFF5348 0.54 +1.1
226,539 + GFF5348 0.54 -0.1
226,539 + GFF5348 0.54 -0.4
226,539 + GFF5348 0.54 +0.3
226,540 - GFF5348 0.54 -0.1
226,540 - GFF5348 0.54 -1.5
226,540 - GFF5348 0.54 -0.2
226,540 - GFF5348 0.54 -1.9
226,540 - GFF5348 0.54 -0.4
226,563 + GFF5348 0.57 -0.1
226,563 + GFF5348 0.57 -2.2
226,563 + GFF5348 0.57 -1.0
226,563 + GFF5348 0.57 -0.4
226,563 + GFF5348 0.57 -1.3
226,563 + GFF5348 0.57 +0.2
226,563 + GFF5348 0.57 +0.1
226,563 + GFF5348 0.57 -0.7
226,564 - GFF5348 0.57 +0.7
226,564 - GFF5348 0.57 -0.3
226,564 - GFF5348 0.57 +0.8
226,564 - GFF5348 0.57 -0.6
226,564 - GFF5348 0.57 -1.0
226,564 - GFF5348 0.57 +0.0
226,564 - GFF5348 0.57 +0.3
226,647 + GFF5348 0.64 -0.1
226,647 + GFF5348 0.64 +0.3
226,648 - GFF5348 0.64 -0.2
226,648 - GFF5348 0.64 -0.3
226,702 - GFF5348 0.69 -1.3
226,702 - GFF5348 0.69 +0.3
226,702 - GFF5348 0.69 +0.5
226,702 - GFF5348 0.69 -0.1
226,702 - GFF5348 0.69 +0.1
226,702 - GFF5348 0.69 +0.1
226,940 + +0.9
226,940 + +1.0
227,032 + +0.1
227,032 + -0.7
227,032 + +0.6
227,032 + +0.2
227,032 + +0.3
227,032 + +0.5
227,032 + -1.4
227,032 + +0.3
227,033 - +0.4
227,033 - +0.8
227,669 + -0.4
227,669 + +0.0
227,670 - -1.3

Or see this region's nucleotide sequence