Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5343 and GFF5344 overlap by 4 nucleotides GFF5344 and GFF5345 are separated by 20 nucleotides GFF5345 and GFF5346 are separated by 32 nucleotides GFF5346 and GFF5347 are separated by 10 nucleotides
GFF5343 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 221,469 to 222,761
GFF5343
GFF5344 - ATP phosphoribosyltransferase (EC 2.4.2.17) => HisGs, at 222,758 to 223,399
GFF5344
GFF5345 - Histidinol dehydrogenase (EC 1.1.1.23), at 223,420 to 224,742
GFF5345
GFF5346 - Putative outer membrane lipoprotein, at 224,775 to 225,587
GFF5346
GFF5347 - hypothetical protein, at 225,598 to 225,948
GFF5347
Position (kb)
223
224
225 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 222.669 kb on + strand at 222.669 kb on + strand at 222.669 kb on + strand at 222.669 kb on + strand at 222.669 kb on + strand at 222.669 kb on + strand at 222.669 kb on + strand at 222.669 kb on + strand at 222.669 kb on + strand at 222.669 kb on + strand at 222.670 kb on - strand at 222.670 kb on - strand at 222.670 kb on - strand at 224.733 kb on + strand at 224.733 kb on + strand at 224.734 kb on - strand at 224.734 kb on - strand at 224.790 kb on + strand at 224.790 kb on + strand at 224.790 kb on + strand at 224.791 kb on - strand at 224.791 kb on - strand at 225.015 kb on + strand, within GFF5346 at 225.015 kb on + strand, within GFF5346 at 225.016 kb on - strand, within GFF5346 at 225.016 kb on - strand, within GFF5346 at 225.016 kb on - strand, within GFF5346 at 225.208 kb on - strand, within GFF5346 at 225.208 kb on - strand, within GFF5346 at 225.243 kb on + strand, within GFF5346 at 225.243 kb on + strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.287 kb on + strand, within GFF5346 at 225.287 kb on + strand, within GFF5346 at 225.287 kb on + strand, within GFF5346 at 225.315 kb on + strand, within GFF5346 at 225.315 kb on + strand, within GFF5346 at 225.315 kb on + strand, within GFF5346 at 225.315 kb on + strand, within GFF5346 at 225.316 kb on - strand, within GFF5346 at 225.316 kb on - strand, within GFF5346 at 225.411 kb on + strand, within GFF5346 at 225.411 kb on + strand, within GFF5346 at 225.411 kb on + strand, within GFF5346 at 225.411 kb on + strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.558 kb on + strand at 225.558 kb on + strand at 225.558 kb on + strand at 225.558 kb on + strand at 225.558 kb on + strand at 225.558 kb on + strand at 225.559 kb on - strand at 225.559 kb on - strand at 225.559 kb on - strand at 225.559 kb on - strand at 225.712 kb on + strand, within GFF5347 at 225.712 kb on + strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.721 kb on + strand, within GFF5347 at 225.721 kb on + strand, within GFF5347 at 225.721 kb on + strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 222,669 + -0.7 222,669 + +0.3 222,669 + +0.3 222,669 + -0.1 222,669 + -0.7 222,669 + -0.7 222,669 + +0.0 222,669 + -1.2 222,669 + +0.0 222,669 + +0.5 222,670 - +0.6 222,670 - -0.3 222,670 - -1.9 224,733 + -0.2 224,733 + -0.1 224,734 - +0.6 224,734 - +1.6 224,790 + +0.4 224,790 + -0.6 224,790 + -1.0 224,791 - +0.5 224,791 - -0.9 225,015 + GFF5346 0.30 -0.4 225,015 + GFF5346 0.30 -0.4 225,016 - GFF5346 0.30 -1.6 225,016 - GFF5346 0.30 -0.1 225,016 - GFF5346 0.30 +0.3 225,208 - GFF5346 0.53 -0.4 225,208 - GFF5346 0.53 +0.1 225,243 + GFF5346 0.58 -1.7 225,243 + GFF5346 0.58 +0.2 225,244 - GFF5346 0.58 +1.3 225,244 - GFF5346 0.58 +0.4 225,244 - GFF5346 0.58 -0.0 225,244 - GFF5346 0.58 +0.5 225,244 - GFF5346 0.58 -0.2 225,244 - GFF5346 0.58 -0.2 225,287 + GFF5346 0.63 +1.0 225,287 + GFF5346 0.63 -0.4 225,287 + GFF5346 0.63 +1.6 225,315 + GFF5346 0.66 -0.6 225,315 + GFF5346 0.66 +0.2 225,315 + GFF5346 0.66 -1.5 225,315 + GFF5346 0.66 -0.2 225,316 - GFF5346 0.67 -1.1 225,316 - GFF5346 0.67 -0.7 225,411 + GFF5346 0.78 +0.6 225,411 + GFF5346 0.78 +1.1 225,411 + GFF5346 0.78 -0.4 225,411 + GFF5346 0.78 -1.2 225,412 - GFF5346 0.78 -1.6 225,412 - GFF5346 0.78 -0.9 225,412 - GFF5346 0.78 -0.5 225,412 - GFF5346 0.78 -0.7 225,412 - GFF5346 0.78 +0.4 225,558 + -1.6 225,558 + +0.6 225,558 + +0.2 225,558 + -1.4 225,558 + +0.4 225,558 + +0.1 225,559 - +0.8 225,559 - +2.1 225,559 - -1.1 225,559 - +0.2 225,712 + GFF5347 0.32 +0.9 225,712 + GFF5347 0.32 +0.1 225,713 - GFF5347 0.33 +0.3 225,713 - GFF5347 0.33 +0.5 225,713 - GFF5347 0.33 -0.3 225,713 - GFF5347 0.33 -0.8 225,713 - GFF5347 0.33 -0.6 225,713 - GFF5347 0.33 -0.0 225,721 + GFF5347 0.35 +1.6 225,721 + GFF5347 0.35 -1.1 225,721 + GFF5347 0.35 +0.1 225,722 - GFF5347 0.35 -0.7 225,722 - GFF5347 0.35 -0.2 225,722 - GFF5347 0.35 +1.9 225,722 - GFF5347 0.35 +0.9 225,722 - GFF5347 0.35 +0.2 225,724 + GFF5347 0.36 -0.7 225,724 + GFF5347 0.36 -2.4 225,724 + GFF5347 0.36 +0.6 225,724 + GFF5347 0.36 +0.2 225,724 + GFF5347 0.36 -0.2 225,724 + GFF5347 0.36 -0.9 225,724 + GFF5347 0.36 -0.4 225,724 + GFF5347 0.36 -1.0 225,725 - GFF5347 0.36 +0.8 225,725 - GFF5347 0.36 +0.2 225,725 - GFF5347 0.36 -0.6 225,725 - GFF5347 0.36 -0.3 225,725 - GFF5347 0.36 -1.5 225,725 - GFF5347 0.36 -0.6 225,725 - GFF5347 0.36 -0.4
Or see this region's nucleotide sequence