Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5249 and GFF5250 are separated by 20 nucleotides GFF5250 and GFF5251 are separated by 395 nucleotides GFF5251 and GFF5252 are separated by 184 nucleotides
GFF5249 - Protein YhfA, at 113,664 to 114,113
GFF5249
GFF5250 - 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, coq7 type, at 114,134 to 114,649
GFF5250
GFF5251 - Outer membrane porin protein 32 precursor, at 115,045 to 116,331
GFF5251
GFF5252 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 116,516 to 117,496
GFF5252
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 114.818 kb on + strand at 114.819 kb on - strand at 114.820 kb on + strand at 114.821 kb on - strand at 114.821 kb on - strand at 114.821 kb on - strand at 114.839 kb on + strand at 114.839 kb on + strand at 114.839 kb on + strand at 114.839 kb on + strand at 114.839 kb on + strand at 114.840 kb on - strand at 114.840 kb on - strand at 114.840 kb on - strand at 114.840 kb on - strand at 114.840 kb on - strand at 114.840 kb on - strand at 115.619 kb on - strand, within GFF5251 at 116.011 kb on + strand, within GFF5251 at 116.330 kb on - strand at 116.346 kb on - strand at 116.346 kb on - strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.488 kb on + strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.489 kb on - strand at 116.529 kb on + strand at 116.529 kb on + strand at 116.529 kb on + strand at 116.529 kb on + strand at 116.529 kb on + strand at 116.530 kb on - strand at 116.530 kb on - strand at 116.592 kb on + strand at 116.592 kb on + strand at 116.592 kb on + strand at 116.592 kb on + strand at 116.592 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.600 kb on + strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.601 kb on - strand at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.867 kb on + strand, within GFF5252 at 116.868 kb on - strand, within GFF5252 at 116.868 kb on - strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.170 kb on + strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252 at 117.171 kb on - strand, within GFF5252
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 114,818 + +0.4 114,819 - -0.4 114,820 + -0.2 114,821 - -0.3 114,821 - +0.5 114,821 - +1.0 114,839 + +0.2 114,839 + -0.2 114,839 + -1.4 114,839 + -0.5 114,839 + -0.1 114,840 - +0.2 114,840 - +2.8 114,840 - +0.4 114,840 - -0.7 114,840 - +0.2 114,840 - -0.4 115,619 - GFF5251 0.45 -2.1 116,011 + GFF5251 0.75 -0.2 116,330 - -0.9 116,346 - +0.2 116,346 - -0.6 116,488 + -0.5 116,488 + -0.2 116,488 + +0.1 116,488 + -0.8 116,488 + -0.6 116,488 + -0.1 116,488 + -0.2 116,488 + -0.1 116,488 + +0.3 116,488 + -1.6 116,488 + +1.2 116,488 + -0.3 116,488 + -0.5 116,488 + -0.4 116,488 + +0.1 116,488 + -0.8 116,488 + +0.2 116,488 + -0.7 116,488 + +0.5 116,489 - -0.2 116,489 - -0.3 116,489 - -0.5 116,489 - -0.7 116,489 - -0.8 116,489 - +1.6 116,489 - -0.4 116,489 - -1.4 116,489 - -0.3 116,489 - -0.1 116,489 - -1.2 116,489 - +0.2 116,489 - +0.0 116,489 - +0.9 116,529 + +0.1 116,529 + +0.2 116,529 + -0.3 116,529 + +0.3 116,529 + -0.2 116,530 - +0.9 116,530 - +0.6 116,592 + +0.3 116,592 + -0.8 116,592 + -0.2 116,592 + +0.1 116,592 + +0.4 116,600 + -0.4 116,600 + +0.0 116,600 + +0.3 116,600 + -0.5 116,600 + -0.5 116,600 + +1.6 116,600 + +0.7 116,600 + +0.2 116,600 + +0.4 116,600 + -0.6 116,601 - -0.5 116,601 - -0.3 116,601 - +0.2 116,601 - -1.1 116,601 - +0.0 116,601 - -2.6 116,601 - -1.6 116,601 - -0.6 116,867 + GFF5252 0.36 -0.6 116,867 + GFF5252 0.36 -0.1 116,867 + GFF5252 0.36 -0.6 116,867 + GFF5252 0.36 +0.4 116,867 + GFF5252 0.36 -0.2 116,867 + GFF5252 0.36 +0.2 116,868 - GFF5252 0.36 -0.3 116,868 - GFF5252 0.36 +0.4 117,170 + GFF5252 0.67 +0.2 117,170 + GFF5252 0.67 +0.4 117,170 + GFF5252 0.67 -2.8 117,170 + GFF5252 0.67 -0.8 117,170 + GFF5252 0.67 -0.2 117,170 + GFF5252 0.67 +0.1 117,170 + GFF5252 0.67 +0.4 117,170 + GFF5252 0.67 -0.9 117,171 - GFF5252 0.67 -0.1 117,171 - GFF5252 0.67 +0.1 117,171 - GFF5252 0.67 -0.5 117,171 - GFF5252 0.67 -0.2 117,171 - GFF5252 0.67 -0.2 117,171 - GFF5252 0.67 +0.9 117,171 - GFF5252 0.67 +0.1 117,171 - GFF5252 0.67 -0.1 117,171 - GFF5252 0.67 -0.7 117,171 - GFF5252 0.67 +0.8
Or see this region's nucleotide sequence