Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5230 and GFF5231 are separated by 121 nucleotides GFF5231 and GFF5232 are separated by 21 nucleotides GFF5232 and GFF5233 are separated by 25 nucleotides
GFF5230 - Xanthine-uracil permease, at 94,465 to 95,880
GFF5230
GFF5231 - Transcriptional regulator, LysR family, at 96,002 to 96,904
GFF5231
GFF5232 - 5'-nucleotidase (EC 3.1.3.5), at 96,926 to 97,825
GFF5232
GFF5233 - General secretion pathway protein F / Type II secretory pathway, component PulF, at 97,851 to 99,089
GFF5233
Position (kb)
96
97 Strain fitness (log2 ratio)
-1
0
1 at 95.771 kb on + strand at 95.772 kb on - strand at 95.772 kb on - strand at 95.865 kb on + strand at 95.953 kb on + strand at 95.954 kb on - strand at 96.459 kb on - strand, within GFF5231 at 96.882 kb on + strand at 96.922 kb on + strand at 96.922 kb on + strand at 97.491 kb on + strand, within GFF5232 at 97.492 kb on - strand, within GFF5232 at 97.666 kb on - strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.719 kb on + strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 97.720 kb on - strand, within GFF5232 at 97.720 kb on - strand, within GFF5232
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 95,771 + +0.1 95,772 - -0.7 95,772 - +0.2 95,865 + +0.0 95,953 + +0.1 95,954 - +0.5 96,459 - GFF5231 0.51 +1.6 96,882 + -0.2 96,922 + -0.2 96,922 + -1.2 97,491 + GFF5232 0.63 +0.4 97,492 - GFF5232 0.63 -1.0 97,666 - GFF5232 0.82 -0.3 97,719 + GFF5232 0.88 +0.4 97,719 + GFF5232 0.88 -0.3 97,719 + GFF5232 0.88 +0.2 97,719 + GFF5232 0.88 +0.4 97,719 + GFF5232 0.88 -0.7 97,720 - GFF5232 0.88 -0.1 97,720 - GFF5232 0.88 -0.0 97,720 - GFF5232 0.88 -0.3 97,720 - GFF5232 0.88 -0.0 97,720 - GFF5232 0.88 +0.3
Or see this region's nucleotide sequence