Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF509 and GFF510 overlap by 4 nucleotides GFF510 and GFF511 overlap by 4 nucleotides GFF511 and GFF512 overlap by 4 nucleotides GFF512 and GFF513 are separated by 44 nucleotides
GFF509 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 550,666 to 551,646
GFF509
GFF510 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 551,643 to 552,371
GFF510
GFF511 - no description, at 552,368 to 552,514
GFF511
GFF512 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 552,511 to 553,278
GFF512
GFF513 - Chitinase (EC 3.2.1.14), at 553,323 to 554,780
GFF513
Position (kb) 552
553 Strain fitness (log2 ratio) -2
-1
0
1
2 at 551.647 kb on - strand at 551.647 kb on - strand at 551.647 kb on - strand at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.118 kb on + strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.119 kb on - strand, within GFF510 at 552.316 kb on + strand at 552.317 kb on - strand at 552.375 kb on + strand at 552.375 kb on + strand at 552.376 kb on - strand at 552.542 kb on + strand at 552.542 kb on + strand at 552.542 kb on + strand at 552.543 kb on - strand at 552.543 kb on - strand at 553.151 kb on + strand, within GFF512 at 553.151 kb on + strand, within GFF512 at 553.151 kb on + strand, within GFF512 at 553.151 kb on + strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.152 kb on - strand, within GFF512 at 553.342 kb on + strand at 553.342 kb on + strand at 553.342 kb on + strand at 553.342 kb on + strand at 553.343 kb on - strand at 553.343 kb on - strand at 553.343 kb on - strand at 553.345 kb on + strand at 553.345 kb on + strand at 553.345 kb on + strand at 553.345 kb on + strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.346 kb on - strand at 553.381 kb on + strand at 553.381 kb on + strand at 553.381 kb on + strand at 553.381 kb on + strand at 553.417 kb on + strand at 553.417 kb on + strand at 553.418 kb on - strand at 553.423 kb on + strand at 553.423 kb on + strand at 553.423 kb on + strand at 553.423 kb on + strand at 553.424 kb on - strand at 553.424 kb on - strand at 553.424 kb on - strand at 553.424 kb on - strand at 553.435 kb on + strand at 553.435 kb on + strand at 553.435 kb on + strand at 553.436 kb on - strand at 553.445 kb on - strand at 553.445 kb on - strand at 553.445 kb on - strand at 553.463 kb on + strand at 553.463 kb on + strand at 553.463 kb on + strand at 553.464 kb on - strand at 553.464 kb on - strand at 553.464 kb on - strand at 553.464 kb on - strand at 553.464 kb on - strand at 553.471 kb on + strand, within GFF513 at 553.471 kb on + strand, within GFF513 at 553.472 kb on - strand, within GFF513 at 553.472 kb on - strand, within GFF513 at 553.472 kb on - strand, within GFF513 at 553.472 kb on - strand, within GFF513 at 553.472 kb on - strand, within GFF513 at 553.472 kb on - strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.486 kb on + strand, within GFF513 at 553.487 kb on - strand, within GFF513 at 553.487 kb on - strand, within GFF513 at 553.487 kb on - strand, within GFF513 at 553.487 kb on - strand, within GFF513 at 553.487 kb on - strand, within GFF513 at 553.487 kb on - strand, within GFF513 at 553.487 kb on - strand, within GFF513 at 553.487 kb on - strand, within GFF513
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 551,647 - -0.6 551,647 - -0.9 551,647 - -0.8 552,118 + GFF510 0.65 -0.9 552,118 + GFF510 0.65 -0.7 552,118 + GFF510 0.65 -1.1 552,118 + GFF510 0.65 +1.2 552,119 - GFF510 0.65 -0.5 552,119 - GFF510 0.65 -0.7 552,119 - GFF510 0.65 -0.4 552,119 - GFF510 0.65 -0.2 552,316 + -0.3 552,317 - -0.6 552,375 + -1.0 552,375 + -1.1 552,376 - -1.0 552,542 + -0.3 552,542 + +0.7 552,542 + -0.1 552,543 - -0.6 552,543 - -2.1 553,151 + GFF512 0.83 -0.7 553,151 + GFF512 0.83 -0.3 553,151 + GFF512 0.83 -0.5 553,151 + GFF512 0.83 +1.8 553,152 - GFF512 0.83 -0.3 553,152 - GFF512 0.83 +0.3 553,152 - GFF512 0.83 +0.4 553,152 - GFF512 0.83 -0.6 553,152 - GFF512 0.83 -0.3 553,152 - GFF512 0.83 +0.0 553,342 + +0.7 553,342 + +0.3 553,342 + +0.9 553,342 + -0.3 553,343 - -1.2 553,343 - -0.1 553,343 - -0.8 553,345 + +0.7 553,345 + +0.3 553,345 + +0.3 553,345 + +0.2 553,346 - +0.0 553,346 - -0.7 553,346 - -0.7 553,346 - -0.4 553,346 - -1.1 553,346 - -0.8 553,346 - -0.8 553,346 - -0.9 553,381 + -0.6 553,381 + +1.7 553,381 + +1.2 553,381 + -0.8 553,417 + -1.1 553,417 + -0.7 553,418 - -0.1 553,423 + -0.1 553,423 + -1.2 553,423 + +0.0 553,423 + -0.1 553,424 - +0.9 553,424 - +0.1 553,424 - +0.1 553,424 - -1.0 553,435 + +2.4 553,435 + -1.1 553,435 + -1.3 553,436 - -0.0 553,445 - -0.5 553,445 - -0.7 553,445 - -0.1 553,463 + -0.7 553,463 + +0.5 553,463 + +0.0 553,464 - -0.8 553,464 - -0.3 553,464 - +0.0 553,464 - +1.4 553,464 - +1.2 553,471 + GFF513 0.10 +0.3 553,471 + GFF513 0.10 -0.2 553,472 - GFF513 0.10 +0.9 553,472 - GFF513 0.10 -0.4 553,472 - GFF513 0.10 -0.4 553,472 - GFF513 0.10 +0.2 553,472 - GFF513 0.10 -0.8 553,472 - GFF513 0.10 -0.0 553,486 + GFF513 0.11 +0.4 553,486 + GFF513 0.11 +1.0 553,486 + GFF513 0.11 -0.5 553,486 + GFF513 0.11 -0.6 553,486 + GFF513 0.11 -0.6 553,486 + GFF513 0.11 -0.7 553,486 + GFF513 0.11 +0.4 553,486 + GFF513 0.11 +0.9 553,486 + GFF513 0.11 -1.1 553,487 - GFF513 0.11 -0.8 553,487 - GFF513 0.11 -0.2 553,487 - GFF513 0.11 -0.3 553,487 - GFF513 0.11 +0.1 553,487 - GFF513 0.11 +0.0 553,487 - GFF513 0.11 +0.4 553,487 - GFF513 0.11 -1.0 553,487 - GFF513 0.11 -0.7
Or see this region's nucleotide sequence