Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5089 and GFF5090 are separated by 145 nucleotides GFF5090 and GFF5091 overlap by 4 nucleotides GFF5091 and GFF5092 overlap by 4 nucleotides GFF5092 and GFF5093 overlap by 4 nucleotides
GFF5089 - Transcriptional regulator, MarR family, at 193,086 to 193,592
GFF5089
GFF5090 - Homoprotocatechuate degradative operon repressor, at 193,738 to 194,175
GFF5090
GFF5091 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 194,172 to 194,804
GFF5091
GFF5092 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 194,801 to 195,583
GFF5092
GFF5093 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 195,580 to 197,043
GFF5093
Position (kb)
194
195 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 193.649 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.649 kb on - strand at 193.650 kb on - strand at 193.653 kb on + strand at 193.653 kb on + strand at 193.653 kb on + strand at 193.653 kb on + strand at 193.682 kb on + strand at 193.682 kb on + strand at 193.683 kb on - strand at 194.081 kb on - strand, within GFF5090 at 194.081 kb on - strand, within GFF5090 at 194.129 kb on - strand, within GFF5090 at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.177 kb on + strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.178 kb on - strand at 194.208 kb on + strand at 194.208 kb on + strand at 194.208 kb on + strand at 194.209 kb on - strand at 194.209 kb on - strand at 194.209 kb on - strand at 194.274 kb on + strand, within GFF5091 at 194.275 kb on - strand, within GFF5091 at 194.822 kb on + strand at 194.822 kb on + strand at 194.822 kb on + strand at 194.823 kb on - strand at 194.823 kb on - strand at 194.823 kb on - strand at 195.116 kb on + strand, within GFF5092 at 195.117 kb on - strand, within GFF5092 at 195.117 kb on - strand, within GFF5092 at 195.117 kb on - strand, within GFF5092 at 195.179 kb on + strand, within GFF5092 at 195.198 kb on - strand, within GFF5092 at 195.215 kb on + strand, within GFF5092 at 195.215 kb on + strand, within GFF5092 at 195.215 kb on + strand, within GFF5092 at 195.216 kb on - strand, within GFF5092 at 195.277 kb on + strand, within GFF5092 at 195.277 kb on + strand, within GFF5092 at 195.425 kb on + strand, within GFF5092 at 195.628 kb on + strand at 195.629 kb on - strand at 195.629 kb on - strand at 195.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 193,649 - +0.5 193,649 - -0.1 193,649 - -0.0 193,649 - +0.0 193,649 - +2.3 193,649 - +2.0 193,650 - -1.6 193,653 + +1.0 193,653 + -0.6 193,653 + +1.0 193,653 + +1.4 193,682 + -0.3 193,682 + -0.2 193,683 - -1.7 194,081 - GFF5090 0.78 -0.6 194,081 - GFF5090 0.78 -2.0 194,129 - GFF5090 0.89 +0.2 194,177 + -0.1 194,177 + -0.6 194,177 + -0.4 194,177 + -0.1 194,177 + +0.4 194,177 + +2.0 194,177 + -0.2 194,177 + -0.8 194,178 - -0.2 194,178 - -0.7 194,178 - -2.0 194,178 - -1.0 194,178 - +1.2 194,208 + -0.7 194,208 + +0.8 194,208 + +0.0 194,209 - -0.7 194,209 - +0.2 194,209 - +0.5 194,274 + GFF5091 0.16 -0.8 194,275 - GFF5091 0.16 -1.0 194,822 + +0.0 194,822 + -0.9 194,822 + -0.5 194,823 - -0.4 194,823 - +0.2 194,823 - -1.5 195,116 + GFF5092 0.40 -1.6 195,117 - GFF5092 0.40 -1.9 195,117 - GFF5092 0.40 +1.2 195,117 - GFF5092 0.40 +0.3 195,179 + GFF5092 0.48 -0.5 195,198 - GFF5092 0.51 +1.8 195,215 + GFF5092 0.53 -0.3 195,215 + GFF5092 0.53 +1.8 195,215 + GFF5092 0.53 -1.4 195,216 - GFF5092 0.53 -0.4 195,277 + GFF5092 0.61 -0.5 195,277 + GFF5092 0.61 +0.3 195,425 + GFF5092 0.80 -0.3 195,628 + -0.7 195,629 - -0.8 195,629 - -0.3 195,629 - -0.6
Or see this region's nucleotide sequence