Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5086 and GFF5087 are separated by 37 nucleotides GFF5087 and GFF5088 are separated by 31 nucleotides
GFF5086 - Glycine betaine transporter OpuD, at 188,981 to 190,495
GFF5086
GFF5087 - DNA repair protein RadA, at 190,533 to 191,927
GFF5087
GFF5088 - hypothetical protein, at 191,959 to 192,978
GFF5088
Position (kb) 190
191
192 Strain fitness (log2 ratio) -2
-1
0
1
2 at 189.577 kb on - strand, within GFF5086 at 189.577 kb on - strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 190.077 kb on + strand, within GFF5086 at 190.078 kb on - strand, within GFF5086 at 190.078 kb on - strand, within GFF5086 at 190.078 kb on - strand, within GFF5086 at 190.117 kb on - strand, within GFF5086 at 190.368 kb on + strand at 190.368 kb on + strand at 190.368 kb on + strand at 190.369 kb on - strand at 191.200 kb on + strand, within GFF5087 at 191.200 kb on + strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.377 kb on + strand, within GFF5087 at 191.377 kb on + strand, within GFF5087 at 191.378 kb on - strand, within GFF5087 at 191.378 kb on - strand, within GFF5087 at 191.378 kb on - strand, within GFF5087 at 191.472 kb on + strand, within GFF5087 at 191.472 kb on + strand, within GFF5087 at 191.473 kb on - strand, within GFF5087 at 191.545 kb on + strand, within GFF5087 at 191.545 kb on + strand, within GFF5087 at 191.545 kb on + strand, within GFF5087 at 191.545 kb on + strand, within GFF5087 at 191.545 kb on + strand, within GFF5087 at 191.546 kb on - strand, within GFF5087 at 191.546 kb on - strand, within GFF5087 at 191.546 kb on - strand, within GFF5087 at 191.546 kb on - strand, within GFF5087 at 191.546 kb on - strand, within GFF5087 at 191.938 kb on + strand at 191.938 kb on + strand at 191.938 kb on + strand at 191.939 kb on - strand at 192.062 kb on + strand, within GFF5088 at 192.062 kb on + strand, within GFF5088 at 192.063 kb on - strand, within GFF5088 at 192.170 kb on + strand, within GFF5088 at 192.170 kb on + strand, within GFF5088 at 192.170 kb on + strand, within GFF5088 at 192.170 kb on + strand, within GFF5088 at 192.171 kb on - strand, within GFF5088 at 192.171 kb on - strand, within GFF5088 at 192.225 kb on + strand, within GFF5088 at 192.225 kb on + strand, within GFF5088 at 192.225 kb on + strand, within GFF5088 at 192.225 kb on + strand, within GFF5088 at 192.226 kb on - strand, within GFF5088 at 192.329 kb on + strand, within GFF5088 at 192.329 kb on + strand, within GFF5088 at 192.329 kb on + strand, within GFF5088 at 192.461 kb on + strand, within GFF5088 at 192.462 kb on - strand, within GFF5088 at 192.608 kb on + strand, within GFF5088 at 192.608 kb on + strand, within GFF5088 at 192.608 kb on + strand, within GFF5088 at 192.609 kb on - strand, within GFF5088 at 192.609 kb on - strand, within GFF5088 at 192.609 kb on - strand, within GFF5088 at 192.609 kb on - strand, within GFF5088 at 192.609 kb on - strand, within GFF5088
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 189,577 - GFF5086 0.39 +0.4 189,577 - GFF5086 0.39 +0.3 189,657 + GFF5086 0.45 -0.2 189,657 + GFF5086 0.45 -0.6 189,657 + GFF5086 0.45 -2.7 189,658 - GFF5086 0.45 +0.2 189,658 - GFF5086 0.45 +0.4 189,658 - GFF5086 0.45 -2.6 189,658 - GFF5086 0.45 -0.6 190,077 + GFF5086 0.72 +0.1 190,078 - GFF5086 0.72 -0.9 190,078 - GFF5086 0.72 -0.0 190,078 - GFF5086 0.72 -0.7 190,117 - GFF5086 0.75 +1.3 190,368 + -0.0 190,368 + -0.8 190,368 + +0.2 190,369 - -0.0 191,200 + GFF5087 0.48 +0.5 191,200 + GFF5087 0.48 -0.0 191,201 - GFF5087 0.48 -0.2 191,201 - GFF5087 0.48 +0.2 191,201 - GFF5087 0.48 -1.1 191,201 - GFF5087 0.48 -0.3 191,201 - GFF5087 0.48 -0.3 191,377 + GFF5087 0.61 +0.4 191,377 + GFF5087 0.61 -0.6 191,378 - GFF5087 0.61 +1.5 191,378 - GFF5087 0.61 +0.3 191,378 - GFF5087 0.61 -1.1 191,472 + GFF5087 0.67 -0.0 191,472 + GFF5087 0.67 -0.3 191,473 - GFF5087 0.67 +0.6 191,545 + GFF5087 0.73 -1.0 191,545 + GFF5087 0.73 -0.1 191,545 + GFF5087 0.73 +0.5 191,545 + GFF5087 0.73 +0.5 191,545 + GFF5087 0.73 -0.1 191,546 - GFF5087 0.73 -0.0 191,546 - GFF5087 0.73 -0.1 191,546 - GFF5087 0.73 +0.1 191,546 - GFF5087 0.73 +0.4 191,546 - GFF5087 0.73 +0.0 191,938 + -0.2 191,938 + +0.2 191,938 + -0.4 191,939 - -0.6 192,062 + GFF5088 0.10 +0.6 192,062 + GFF5088 0.10 -1.6 192,063 - GFF5088 0.10 -0.4 192,170 + GFF5088 0.21 +0.2 192,170 + GFF5088 0.21 -0.3 192,170 + GFF5088 0.21 +1.8 192,170 + GFF5088 0.21 +0.3 192,171 - GFF5088 0.21 -0.0 192,171 - GFF5088 0.21 +0.2 192,225 + GFF5088 0.26 +0.7 192,225 + GFF5088 0.26 -0.9 192,225 + GFF5088 0.26 -0.0 192,225 + GFF5088 0.26 -0.2 192,226 - GFF5088 0.26 +0.1 192,329 + GFF5088 0.36 -0.7 192,329 + GFF5088 0.36 +0.5 192,329 + GFF5088 0.36 -0.2 192,461 + GFF5088 0.49 -0.5 192,462 - GFF5088 0.49 -0.9 192,608 + GFF5088 0.64 -0.7 192,608 + GFF5088 0.64 +0.5 192,608 + GFF5088 0.64 +0.0 192,609 - GFF5088 0.64 +0.9 192,609 - GFF5088 0.64 -0.4 192,609 - GFF5088 0.64 +0.1 192,609 - GFF5088 0.64 +0.4 192,609 - GFF5088 0.64 +0.8
Or see this region's nucleotide sequence