Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5084 and GFF5085 are separated by 184 nucleotides GFF5085 and GFF5086 are separated by 62 nucleotides GFF5086 and GFF5087 are separated by 37 nucleotides
GFF5084 - FIG00933367: hypothetical protein, at 187,841 to 188,278
GFF5084
GFF5085 - hypothetical protein, at 188,463 to 188,918
GFF5085
GFF5086 - Glycine betaine transporter OpuD, at 188,981 to 190,495
GFF5086
GFF5087 - DNA repair protein RadA, at 190,533 to 191,927
GFF5087
Position (kb) 188
189
190
191 Strain fitness (log2 ratio) -2
-1
0
1 at 188.091 kb on + strand, within GFF5084 at 188.091 kb on + strand, within GFF5084 at 188.092 kb on - strand, within GFF5084 at 188.092 kb on - strand, within GFF5084 at 188.092 kb on - strand, within GFF5084 at 188.343 kb on + strand at 188.378 kb on + strand at 188.378 kb on + strand at 188.462 kb on + strand at 188.462 kb on + strand at 188.462 kb on + strand at 188.462 kb on + strand at 188.462 kb on + strand at 188.463 kb on - strand at 188.541 kb on + strand, within GFF5085 at 188.541 kb on + strand, within GFF5085 at 188.541 kb on + strand, within GFF5085 at 188.541 kb on + strand, within GFF5085 at 188.541 kb on + strand, within GFF5085 at 188.542 kb on - strand, within GFF5085 at 188.542 kb on - strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.592 kb on + strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.593 kb on - strand, within GFF5085 at 188.640 kb on + strand, within GFF5085 at 188.641 kb on - strand, within GFF5085 at 188.667 kb on + strand, within GFF5085 at 188.835 kb on + strand, within GFF5085 at 188.835 kb on + strand, within GFF5085 at 188.835 kb on + strand, within GFF5085 at 188.836 kb on - strand, within GFF5085 at 188.925 kb on + strand at 188.926 kb on - strand at 188.926 kb on - strand at 188.926 kb on - strand at 189.131 kb on + strand at 189.131 kb on + strand at 189.131 kb on + strand at 189.131 kb on + strand at 189.131 kb on + strand at 189.132 kb on - strand at 189.132 kb on - strand at 189.368 kb on + strand, within GFF5086 at 189.368 kb on + strand, within GFF5086 at 189.368 kb on + strand, within GFF5086 at 189.368 kb on + strand, within GFF5086 at 189.368 kb on + strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.369 kb on - strand, within GFF5086 at 189.577 kb on - strand, within GFF5086 at 189.577 kb on - strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.657 kb on + strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 189.658 kb on - strand, within GFF5086 at 190.077 kb on + strand, within GFF5086 at 190.078 kb on - strand, within GFF5086 at 190.078 kb on - strand, within GFF5086 at 190.078 kb on - strand, within GFF5086 at 190.117 kb on - strand, within GFF5086 at 190.368 kb on + strand at 190.368 kb on + strand at 190.368 kb on + strand at 190.369 kb on - strand at 191.200 kb on + strand, within GFF5087 at 191.200 kb on + strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.201 kb on - strand, within GFF5087 at 191.377 kb on + strand, within GFF5087 at 191.377 kb on + strand, within GFF5087 at 191.378 kb on - strand, within GFF5087 at 191.378 kb on - strand, within GFF5087 at 191.378 kb on - strand, within GFF5087 at 191.472 kb on + strand, within GFF5087 at 191.472 kb on + strand, within GFF5087 at 191.473 kb on - strand, within GFF5087
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 188,091 + GFF5084 0.57 -2.5 188,091 + GFF5084 0.57 -0.5 188,092 - GFF5084 0.57 -0.4 188,092 - GFF5084 0.57 -0.4 188,092 - GFF5084 0.57 -0.4 188,343 + -2.0 188,378 + -0.4 188,378 + -1.4 188,462 + -0.8 188,462 + +0.0 188,462 + -0.1 188,462 + -0.4 188,462 + -0.5 188,463 - -0.6 188,541 + GFF5085 0.17 -0.7 188,541 + GFF5085 0.17 -1.3 188,541 + GFF5085 0.17 -0.0 188,541 + GFF5085 0.17 -0.5 188,541 + GFF5085 0.17 -0.3 188,542 - GFF5085 0.17 -0.4 188,542 - GFF5085 0.17 -1.0 188,592 + GFF5085 0.28 -1.2 188,592 + GFF5085 0.28 -0.1 188,592 + GFF5085 0.28 -0.4 188,592 + GFF5085 0.28 -1.0 188,592 + GFF5085 0.28 -0.6 188,592 + GFF5085 0.28 -1.6 188,592 + GFF5085 0.28 -0.2 188,592 + GFF5085 0.28 -0.7 188,592 + GFF5085 0.28 -1.2 188,592 + GFF5085 0.28 -1.6 188,592 + GFF5085 0.28 +0.9 188,593 - GFF5085 0.29 -0.4 188,593 - GFF5085 0.29 -0.8 188,593 - GFF5085 0.29 +0.1 188,593 - GFF5085 0.29 -0.7 188,593 - GFF5085 0.29 -0.2 188,593 - GFF5085 0.29 +0.1 188,640 + GFF5085 0.39 +0.2 188,641 - GFF5085 0.39 -0.3 188,667 + GFF5085 0.45 +0.6 188,835 + GFF5085 0.82 -1.9 188,835 + GFF5085 0.82 +0.3 188,835 + GFF5085 0.82 -1.1 188,836 - GFF5085 0.82 -0.5 188,925 + -1.0 188,926 - -0.1 188,926 - +0.6 188,926 - -0.8 189,131 + -1.1 189,131 + +0.5 189,131 + +1.0 189,131 + -0.4 189,131 + -0.3 189,132 - -1.4 189,132 - -1.6 189,368 + GFF5086 0.26 +0.3 189,368 + GFF5086 0.26 -1.1 189,368 + GFF5086 0.26 +1.4 189,368 + GFF5086 0.26 +0.3 189,368 + GFF5086 0.26 -0.2 189,369 - GFF5086 0.26 +0.1 189,369 - GFF5086 0.26 +0.5 189,369 - GFF5086 0.26 -1.4 189,369 - GFF5086 0.26 -0.7 189,369 - GFF5086 0.26 -1.2 189,577 - GFF5086 0.39 +0.4 189,577 - GFF5086 0.39 +0.3 189,657 + GFF5086 0.45 -0.2 189,657 + GFF5086 0.45 -0.6 189,657 + GFF5086 0.45 -2.7 189,658 - GFF5086 0.45 +0.2 189,658 - GFF5086 0.45 +0.4 189,658 - GFF5086 0.45 -2.6 189,658 - GFF5086 0.45 -0.6 190,077 + GFF5086 0.72 +0.1 190,078 - GFF5086 0.72 -0.9 190,078 - GFF5086 0.72 -0.0 190,078 - GFF5086 0.72 -0.7 190,117 - GFF5086 0.75 +1.3 190,368 + -0.0 190,368 + -0.8 190,368 + +0.2 190,369 - -0.0 191,200 + GFF5087 0.48 +0.5 191,200 + GFF5087 0.48 -0.0 191,201 - GFF5087 0.48 -0.2 191,201 - GFF5087 0.48 +0.2 191,201 - GFF5087 0.48 -1.1 191,201 - GFF5087 0.48 -0.3 191,201 - GFF5087 0.48 -0.3 191,377 + GFF5087 0.61 +0.4 191,377 + GFF5087 0.61 -0.6 191,378 - GFF5087 0.61 +1.5 191,378 - GFF5087 0.61 +0.3 191,378 - GFF5087 0.61 -1.1 191,472 + GFF5087 0.67 -0.0 191,472 + GFF5087 0.67 -0.3 191,473 - GFF5087 0.67 +0.6
Or see this region's nucleotide sequence