Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5059 and GFF5060 are separated by 86 nucleotides GFF5060 and GFF5061 are separated by 215 nucleotides GFF5061 and GFF5062 overlap by 1 nucleotides
GFF5059 - Rod shape-determining protein MreC, at 161,053 to 162,012
GFF5059
GFF5060 - Rod shape-determining protein MreB, at 162,099 to 163,142
GFF5060
GFF5061 - Aspartyl-tRNA(Asn) amidotransferase subunit C (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit C (EC 6.3.5.7), at 163,358 to 163,657
GFF5061
GFF5062 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 163,657 to 165,171
GFF5062
Position (kb)
162
163
164 Strain fitness (log2 ratio)
-1
0
1 at 162.046 kb on - strand at 162.100 kb on + strand at 162.101 kb on - strand at 162.101 kb on - strand at 162.101 kb on - strand at 162.101 kb on - strand at 163.278 kb on + strand at 163.278 kb on + strand at 163.302 kb on + strand at 163.302 kb on + strand at 163.357 kb on + strand at 163.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 162,046 - -0.5 162,100 + -0.2 162,101 - +0.8 162,101 - +0.2 162,101 - -0.2 162,101 - -0.3 163,278 + -0.5 163,278 + -0.3 163,302 + -0.6 163,302 + +0.3 163,357 + +0.3 163,357 + -0.0
Or see this region's nucleotide sequence