Strain Fitness in Variovorax sp. SCN45 around GFF5060

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5059 and GFF5060 are separated by 86 nucleotidesGFF5060 and GFF5061 are separated by 215 nucleotidesGFF5061 and GFF5062 overlap by 1 nucleotides GFF5059 - Rod shape-determining protein MreC, at 161,053 to 162,012 GFF5059 GFF5060 - Rod shape-determining protein MreB, at 162,099 to 163,142 GFF5060 GFF5061 - Aspartyl-tRNA(Asn) amidotransferase subunit C (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit C (EC 6.3.5.7), at 163,358 to 163,657 GFF5061 GFF5062 - Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7), at 163,657 to 165,171 GFF5062 Position (kb) 162 163 164Strain fitness (log2 ratio) -1 0 1at 162.046 kb on - strandat 162.100 kb on + strandat 162.101 kb on - strandat 162.101 kb on - strandat 162.101 kb on - strandat 162.101 kb on - strandat 163.278 kb on + strandat 163.278 kb on + strandat 163.302 kb on + strandat 163.302 kb on + strandat 163.357 kb on + strandat 163.357 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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162,046 - -0.5
162,100 + -0.2
162,101 - +0.8
162,101 - +0.2
162,101 - -0.2
162,101 - -0.3
163,278 + -0.5
163,278 + -0.3
163,302 + -0.6
163,302 + +0.3
163,357 + +0.3
163,357 + -0.0

Or see this region's nucleotide sequence