Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4958 and GFF4959 overlap by 4 nucleotides GFF4959 and GFF4960 overlap by 4 nucleotides GFF4960 and GFF4961 overlap by 4 nucleotides GFF4961 and GFF4962 are separated by 7 nucleotides
GFF4958 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 58,886 to 59,806
GFF4958
GFF4959 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 59,803 to 60,792
GFF4959
GFF4960 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,789 to 61,544
GFF4960
GFF4961 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 61,541 to 62,260
GFF4961
GFF4962 - Multidrug resistance transporter, Bcr/CflA family, at 62,268 to 63,476
GFF4962
Position (kb) 60
61
62 Strain fitness (log2 ratio) -1
0
1
2 at 59.824 kb on + strand at 59.824 kb on + strand at 59.825 kb on - strand at 59.899 kb on + strand at 59.899 kb on + strand at 59.899 kb on + strand at 60.019 kb on + strand, within GFF4959 at 60.145 kb on + strand, within GFF4959 at 60.146 kb on - strand, within GFF4959 at 60.184 kb on + strand, within GFF4959 at 60.185 kb on - strand, within GFF4959 at 60.230 kb on - strand, within GFF4959 at 62.132 kb on + strand, within GFF4961 at 62.132 kb on + strand, within GFF4961 at 62.132 kb on + strand, within GFF4961 at 62.132 kb on + strand, within GFF4961 at 62.133 kb on - strand, within GFF4961 at 62.133 kb on - strand, within GFF4961 at 62.258 kb on + strand at 62.259 kb on - strand at 62.259 kb on - strand at 62.259 kb on - strand at 62.524 kb on + strand, within GFF4962
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 59,824 + +1.0 59,824 + -0.5 59,825 - -0.0 59,899 + -0.6 59,899 + +1.8 59,899 + +0.1 60,019 + GFF4959 0.22 +0.5 60,145 + GFF4959 0.35 -0.4 60,146 - GFF4959 0.35 -0.4 60,184 + GFF4959 0.38 -0.1 60,185 - GFF4959 0.39 -0.1 60,230 - GFF4959 0.43 +0.0 62,132 + GFF4961 0.82 -1.2 62,132 + GFF4961 0.82 +1.3 62,132 + GFF4961 0.82 -0.4 62,132 + GFF4961 0.82 -0.5 62,133 - GFF4961 0.82 +0.1 62,133 - GFF4961 0.82 -0.3 62,258 + -1.5 62,259 - -0.1 62,259 - -0.6 62,259 - +0.2 62,524 + GFF4962 0.21 -0.2
Or see this region's nucleotide sequence