Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4884 and GFF4883 overlap by 27 nucleotides GFF4883 and GFF4885 overlap by 1 nucleotides GFF4885 and GFF4886 are separated by 147 nucleotides GFF4886 and GFF4887 are separated by 142 nucleotides GFF4887 and GFF4888 are separated by 63 nucleotides
GFF4884 - UPF0380 proteins YafZ and homologs, at 251,947 to 252,771
GFF4884
GFF4883 - Translation-disabling ACNase RloC, at 252,745 to 253,371
GFF4883
GFF4885 - FIG00582571: hypothetical protein, at 253,371 to 253,559
GFF4885
GFF4886 - hypothetical protein, at 253,707 to 254,006
GFF4886
GFF4887 - FIG00582580: hypothetical protein, at 254,149 to 254,427
GFF4887
GFF4888 - Mlr6156 protein, at 254,491 to 254,841
GFF4888
Position (kb)
253
254 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 252.438 kb on - strand, within GFF4884 at 252.438 kb on - strand, within GFF4884 at 252.480 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.635 kb on + strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 252.636 kb on - strand, within GFF4884 at 253.035 kb on + strand, within GFF4883 at 253.035 kb on + strand, within GFF4883 at 253.036 kb on - strand, within GFF4883 at 253.227 kb on + strand, within GFF4883 at 253.228 kb on - strand, within GFF4883 at 253.228 kb on - strand, within GFF4883 at 253.228 kb on - strand, within GFF4883 at 253.228 kb on - strand, within GFF4883 at 253.305 kb on + strand, within GFF4883 at 253.305 kb on + strand, within GFF4883 at 253.305 kb on + strand, within GFF4883 at 253.305 kb on + strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.306 kb on - strand, within GFF4883 at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.345 kb on + strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.346 kb on - strand at 253.354 kb on + strand at 253.354 kb on + strand at 253.354 kb on + strand at 253.354 kb on + strand at 253.354 kb on + strand at 253.355 kb on - strand at 253.355 kb on - strand at 253.355 kb on - strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.557 kb on + strand at 253.558 kb on - strand at 253.558 kb on - strand at 253.558 kb on - strand at 253.674 kb on + strand at 253.674 kb on + strand at 253.674 kb on + strand at 253.675 kb on - strand at 253.675 kb on - strand at 253.896 kb on + strand, within GFF4886 at 253.896 kb on + strand, within GFF4886 at 253.896 kb on + strand, within GFF4886 at 253.969 kb on - strand, within GFF4886 at 253.969 kb on - strand, within GFF4886 at 253.969 kb on - strand, within GFF4886 at 254.004 kb on + strand at 254.004 kb on + strand at 254.004 kb on + strand at 254.004 kb on + strand at 254.004 kb on + strand at 254.004 kb on + strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.005 kb on - strand at 254.219 kb on + strand, within GFF4887 at 254.220 kb on - strand, within GFF4887 at 254.220 kb on - strand, within GFF4887 at 254.237 kb on + strand, within GFF4887 at 254.238 kb on - strand, within GFF4887 at 254.238 kb on - strand, within GFF4887 at 254.238 kb on - strand, within GFF4887 at 254.264 kb on + strand, within GFF4887 at 254.265 kb on - strand, within GFF4887 at 254.265 kb on - strand, within GFF4887 at 254.310 kb on + strand, within GFF4887 at 254.310 kb on + strand, within GFF4887 at 254.311 kb on - strand, within GFF4887 at 254.311 kb on - strand, within GFF4887 at 254.311 kb on - strand, within GFF4887 at 254.311 kb on - strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.312 kb on + strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.313 kb on - strand, within GFF4887 at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.408 kb on + strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.409 kb on - strand at 254.531 kb on + strand, within GFF4888 at 254.531 kb on + strand, within GFF4888 at 254.531 kb on + strand, within GFF4888 at 254.532 kb on - strand, within GFF4888 at 254.532 kb on - strand, within GFF4888
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 252,438 - GFF4884 0.60 -0.1 252,438 - GFF4884 0.60 +0.4 252,480 + GFF4884 0.65 -0.1 252,635 + GFF4884 0.83 -0.3 252,635 + GFF4884 0.83 +0.4 252,635 + GFF4884 0.83 -1.9 252,635 + GFF4884 0.83 +0.4 252,635 + GFF4884 0.83 +0.1 252,635 + GFF4884 0.83 -0.5 252,635 + GFF4884 0.83 +0.6 252,635 + GFF4884 0.83 +0.2 252,635 + GFF4884 0.83 +0.3 252,635 + GFF4884 0.83 -0.6 252,635 + GFF4884 0.83 -0.1 252,635 + GFF4884 0.83 +0.1 252,635 + GFF4884 0.83 +0.8 252,635 + GFF4884 0.83 +1.0 252,635 + GFF4884 0.83 -1.1 252,636 - GFF4884 0.84 -0.7 252,636 - GFF4884 0.84 +0.8 252,636 - GFF4884 0.84 -0.1 252,636 - GFF4884 0.84 +0.1 252,636 - GFF4884 0.84 -0.6 252,636 - GFF4884 0.84 -1.1 252,636 - GFF4884 0.84 -0.2 252,636 - GFF4884 0.84 -0.7 252,636 - GFF4884 0.84 -0.6 252,636 - GFF4884 0.84 -0.9 252,636 - GFF4884 0.84 +0.7 253,035 + GFF4883 0.46 -1.1 253,035 + GFF4883 0.46 -1.2 253,036 - GFF4883 0.46 -0.4 253,227 + GFF4883 0.77 -0.3 253,228 - GFF4883 0.77 -0.1 253,228 - GFF4883 0.77 -0.5 253,228 - GFF4883 0.77 +1.2 253,228 - GFF4883 0.77 +0.4 253,305 + GFF4883 0.89 -0.1 253,305 + GFF4883 0.89 -0.5 253,305 + GFF4883 0.89 +0.4 253,305 + GFF4883 0.89 -0.1 253,306 - GFF4883 0.89 +0.6 253,306 - GFF4883 0.89 +0.1 253,306 - GFF4883 0.89 -1.1 253,306 - GFF4883 0.89 +0.3 253,306 - GFF4883 0.89 +1.1 253,306 - GFF4883 0.89 +0.5 253,306 - GFF4883 0.89 +0.0 253,306 - GFF4883 0.89 -0.9 253,345 + +0.1 253,345 + +2.0 253,345 + +0.8 253,345 + -0.7 253,345 + +0.8 253,345 + +1.6 253,345 + -1.9 253,345 + -0.4 253,345 + +0.8 253,345 + -0.1 253,345 + -0.2 253,345 + +0.8 253,345 + +0.3 253,345 + +0.2 253,345 + -1.2 253,345 + -0.3 253,345 + +0.7 253,345 + -0.0 253,345 + +0.3 253,345 + -1.0 253,345 + -0.0 253,345 + +0.2 253,345 + -0.0 253,345 + -0.3 253,345 + -0.1 253,345 + -0.0 253,346 - -1.0 253,346 - +0.0 253,346 - -1.1 253,346 - -0.1 253,346 - -1.2 253,346 - +1.9 253,346 - -0.5 253,346 - -0.2 253,346 - -0.3 253,346 - +0.2 253,346 - -1.1 253,346 - +0.2 253,346 - -0.6 253,346 - +1.8 253,346 - +1.3 253,346 - +0.1 253,346 - +0.1 253,346 - -0.3 253,346 - -0.4 253,346 - +0.3 253,354 + -0.7 253,354 + -0.5 253,354 + -0.1 253,354 + +0.2 253,354 + -0.1 253,355 - -2.2 253,355 - +0.6 253,355 - +1.0 253,557 + +0.3 253,557 + +0.3 253,557 + +0.7 253,557 + -0.7 253,557 + -0.3 253,557 + -1.0 253,557 + +0.4 253,557 + +0.4 253,558 - +0.6 253,558 - +1.8 253,558 - -0.1 253,674 + +0.0 253,674 + -0.3 253,674 + -0.0 253,675 - -0.2 253,675 - -0.3 253,896 + GFF4886 0.63 +2.3 253,896 + GFF4886 0.63 -0.1 253,896 + GFF4886 0.63 -0.4 253,969 - GFF4886 0.87 -0.0 253,969 - GFF4886 0.87 -1.6 253,969 - GFF4886 0.87 +0.5 254,004 + +0.4 254,004 + -0.6 254,004 + -0.3 254,004 + -0.4 254,004 + +1.3 254,004 + -1.3 254,005 - +1.3 254,005 - -1.1 254,005 - -2.5 254,005 - -0.0 254,005 - -0.5 254,005 - -0.7 254,005 - +0.3 254,005 - -1.5 254,219 + GFF4887 0.25 -0.3 254,220 - GFF4887 0.25 -0.5 254,220 - GFF4887 0.25 -0.4 254,237 + GFF4887 0.32 +0.2 254,238 - GFF4887 0.32 +0.9 254,238 - GFF4887 0.32 +1.1 254,238 - GFF4887 0.32 +0.3 254,264 + GFF4887 0.41 +1.3 254,265 - GFF4887 0.42 -1.4 254,265 - GFF4887 0.42 -0.9 254,310 + GFF4887 0.58 +0.5 254,310 + GFF4887 0.58 -1.2 254,311 - GFF4887 0.58 +0.7 254,311 - GFF4887 0.58 +0.2 254,311 - GFF4887 0.58 +0.7 254,311 - GFF4887 0.58 +0.5 254,312 + GFF4887 0.58 +0.3 254,312 + GFF4887 0.58 -0.1 254,312 + GFF4887 0.58 +0.2 254,312 + GFF4887 0.58 -0.1 254,312 + GFF4887 0.58 -1.5 254,312 + GFF4887 0.58 +1.0 254,312 + GFF4887 0.58 -1.0 254,312 + GFF4887 0.58 +0.6 254,312 + GFF4887 0.58 +0.1 254,312 + GFF4887 0.58 -0.7 254,312 + GFF4887 0.58 +0.2 254,312 + GFF4887 0.58 -0.3 254,312 + GFF4887 0.58 +0.2 254,312 + GFF4887 0.58 -0.1 254,312 + GFF4887 0.58 -0.1 254,312 + GFF4887 0.58 -2.4 254,312 + GFF4887 0.58 +0.4 254,312 + GFF4887 0.58 -0.4 254,312 + GFF4887 0.58 -0.4 254,312 + GFF4887 0.58 +0.7 254,312 + GFF4887 0.58 +0.0 254,313 - GFF4887 0.59 -0.2 254,313 - GFF4887 0.59 +0.4 254,313 - GFF4887 0.59 -1.5 254,313 - GFF4887 0.59 +1.0 254,313 - GFF4887 0.59 -0.2 254,313 - GFF4887 0.59 -0.6 254,313 - GFF4887 0.59 +0.2 254,313 - GFF4887 0.59 +0.3 254,313 - GFF4887 0.59 -0.1 254,408 + -0.4 254,408 + +3.3 254,408 + -1.0 254,408 + -0.2 254,408 + -1.0 254,408 + -0.4 254,408 + -0.5 254,408 + +1.6 254,408 + +0.7 254,408 + +0.2 254,408 + +2.6 254,408 + +0.5 254,408 + -0.2 254,408 + +0.0 254,408 + -0.4 254,408 + -0.1 254,408 + +0.1 254,408 + +0.2 254,408 + +0.4 254,408 + -0.2 254,408 + +1.8 254,408 + +0.2 254,409 - +2.3 254,409 - -1.8 254,409 - +0.0 254,409 - -0.7 254,409 - +0.2 254,409 - +0.1 254,409 - +0.3 254,409 - +0.8 254,409 - -0.6 254,409 - -0.6 254,409 - +0.4 254,409 - -0.1 254,409 - +0.7 254,409 - -0.0 254,409 - -0.4 254,409 - +0.4 254,531 + GFF4888 0.11 +0.0 254,531 + GFF4888 0.11 -1.9 254,531 + GFF4888 0.11 +0.4 254,532 - GFF4888 0.12 -1.6 254,532 - GFF4888 0.12 +0.4
Or see this region's nucleotide sequence