Strain Fitness in Variovorax sp. SCN45 around GFF4844

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4842 and GFF4843 overlap by 4 nucleotidesGFF4843 and GFF4844 are separated by 18 nucleotidesGFF4844 and GFF4845 are separated by 12 nucleotidesGFF4845 and GFF4846 overlap by 4 nucleotides GFF4842 - Conjugative transfer protein TrbG, at 211,805 to 212,800 GFF4842 GFF4843 - Conjugative transfer protein TrbF, at 212,797 to 213,501 GFF4843 GFF4844 - Conjugative transfer protein TrbL, at 213,520 to 214,872 GFF4844 GFF4845 - Conjugative transfer protein TrbJ, at 214,885 to 215,640 GFF4845 GFF4846 - Conjugative transfer protein TrbE, at 215,637 to 218,114 GFF4846 Position (kb) 213 214 215Strain fitness (log2 ratio) -2 -1 0 1at 212.682 kb on - strand, within GFF4842at 212.682 kb on - strand, within GFF4842at 212.783 kb on - strandat 212.855 kb on + strandat 212.855 kb on + strandat 212.855 kb on + strandat 212.855 kb on + strandat 212.855 kb on + strandat 212.855 kb on + strandat 212.855 kb on + strandat 212.856 kb on - strandat 212.856 kb on - strandat 212.856 kb on - strandat 212.856 kb on - strandat 212.856 kb on - strandat 213.081 kb on - strand, within GFF4843at 213.117 kb on - strand, within GFF4843at 213.122 kb on + strand, within GFF4843at 213.122 kb on + strand, within GFF4843at 213.123 kb on - strand, within GFF4843at 213.291 kb on - strand, within GFF4843at 213.291 kb on - strand, within GFF4843at 213.388 kb on - strand, within GFF4843at 213.388 kb on - strand, within GFF4843at 213.388 kb on - strand, within GFF4843at 213.388 kb on - strand, within GFF4843at 213.473 kb on + strandat 213.600 kb on + strandat 213.600 kb on + strandat 213.771 kb on - strand, within GFF4844at 213.771 kb on - strand, within GFF4844at 214.183 kb on + strand, within GFF4844at 214.183 kb on + strand, within GFF4844at 214.183 kb on + strand, within GFF4844at 214.183 kb on + strand, within GFF4844at 214.184 kb on - strand, within GFF4844at 214.184 kb on - strand, within GFF4844at 214.285 kb on + strand, within GFF4844at 214.286 kb on - strand, within GFF4844at 214.646 kb on + strand, within GFF4844at 214.646 kb on + strand, within GFF4844at 214.646 kb on + strand, within GFF4844at 214.647 kb on - strand, within GFF4844at 214.647 kb on - strand, within GFF4844at 214.647 kb on - strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.664 kb on + strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.665 kb on - strand, within GFF4844at 214.666 kb on + strand, within GFF4844at 214.666 kb on + strand, within GFF4844at 214.666 kb on + strand, within GFF4844at 214.666 kb on + strand, within GFF4844at 214.666 kb on + strand, within GFF4844at 214.666 kb on + strand, within GFF4844at 214.666 kb on + strand, within GFF4844at 214.667 kb on - strand, within GFF4844at 214.667 kb on - strand, within GFF4844at 214.667 kb on - strand, within GFF4844at 214.823 kb on + strandat 214.824 kb on - strandat 214.895 kb on + strandat 214.896 kb on - strandat 214.919 kb on + strandat 214.919 kb on + strandat 214.920 kb on - strandat 215.243 kb on + strand, within GFF4845at 215.243 kb on + strand, within GFF4845at 215.243 kb on + strand, within GFF4845at 215.244 kb on - strand, within GFF4845at 215.244 kb on - strand, within GFF4845at 215.244 kb on - strand, within GFF4845at 215.244 kb on - strand, within GFF4845at 215.273 kb on + strand, within GFF4845at 215.273 kb on + strand, within GFF4845at 215.273 kb on + strand, within GFF4845at 215.273 kb on + strand, within GFF4845at 215.273 kb on + strand, within GFF4845at 215.274 kb on - strand, within GFF4845at 215.274 kb on - strand, within GFF4845at 215.274 kb on - strand, within GFF4845at 215.274 kb on - strand, within GFF4845at 215.286 kb on - strand, within GFF4845at 215.324 kb on + strand, within GFF4845

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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212,682 - GFF4842 0.88 -1.2
212,682 - GFF4842 0.88 -0.5
212,783 - -0.4
212,855 + -0.8
212,855 + -1.3
212,855 + -0.6
212,855 + -0.4
212,855 + +0.1
212,855 + -1.0
212,855 + -0.2
212,856 - +0.7
212,856 - +0.6
212,856 - +0.3
212,856 - -0.3
212,856 - -0.7
213,081 - GFF4843 0.40 -0.3
213,117 - GFF4843 0.45 -0.5
213,122 + GFF4843 0.46 +0.1
213,122 + GFF4843 0.46 -0.7
213,123 - GFF4843 0.46 +0.0
213,291 - GFF4843 0.70 +0.3
213,291 - GFF4843 0.70 -0.0
213,388 - GFF4843 0.84 +0.4
213,388 - GFF4843 0.84 -1.7
213,388 - GFF4843 0.84 +0.6
213,388 - GFF4843 0.84 +0.7
213,473 + -0.3
213,600 + +0.3
213,600 + -0.4
213,771 - GFF4844 0.19 +0.7
213,771 - GFF4844 0.19 -0.5
214,183 + GFF4844 0.49 -0.7
214,183 + GFF4844 0.49 -0.5
214,183 + GFF4844 0.49 +0.1
214,183 + GFF4844 0.49 -0.1
214,184 - GFF4844 0.49 +0.5
214,184 - GFF4844 0.49 -0.2
214,285 + GFF4844 0.57 -2.2
214,286 - GFF4844 0.57 +0.3
214,646 + GFF4844 0.83 +0.3
214,646 + GFF4844 0.83 -0.3
214,646 + GFF4844 0.83 -1.7
214,647 - GFF4844 0.83 +0.8
214,647 - GFF4844 0.83 +0.4
214,647 - GFF4844 0.83 -0.8
214,664 + GFF4844 0.85 -0.0
214,664 + GFF4844 0.85 -1.2
214,664 + GFF4844 0.85 -0.7
214,664 + GFF4844 0.85 -0.2
214,664 + GFF4844 0.85 +0.1
214,664 + GFF4844 0.85 +0.3
214,664 + GFF4844 0.85 +0.1
214,664 + GFF4844 0.85 -0.1
214,664 + GFF4844 0.85 -1.1
214,664 + GFF4844 0.85 -0.5
214,664 + GFF4844 0.85 +0.0
214,664 + GFF4844 0.85 +0.2
214,664 + GFF4844 0.85 -1.3
214,665 - GFF4844 0.85 -0.2
214,665 - GFF4844 0.85 -0.9
214,665 - GFF4844 0.85 -1.2
214,665 - GFF4844 0.85 +0.3
214,665 - GFF4844 0.85 +1.7
214,665 - GFF4844 0.85 -0.4
214,665 - GFF4844 0.85 -0.1
214,665 - GFF4844 0.85 +0.8
214,665 - GFF4844 0.85 +0.2
214,665 - GFF4844 0.85 +0.8
214,665 - GFF4844 0.85 +0.2
214,665 - GFF4844 0.85 +1.4
214,665 - GFF4844 0.85 -0.6
214,665 - GFF4844 0.85 +1.3
214,665 - GFF4844 0.85 -0.2
214,665 - GFF4844 0.85 +0.3
214,665 - GFF4844 0.85 -0.9
214,665 - GFF4844 0.85 +0.2
214,665 - GFF4844 0.85 -0.5
214,665 - GFF4844 0.85 -1.8
214,665 - GFF4844 0.85 -0.8
214,665 - GFF4844 0.85 -0.1
214,665 - GFF4844 0.85 -0.5
214,665 - GFF4844 0.85 -1.5
214,665 - GFF4844 0.85 +1.3
214,666 + GFF4844 0.85 -1.2
214,666 + GFF4844 0.85 +1.3
214,666 + GFF4844 0.85 -0.5
214,666 + GFF4844 0.85 +1.3
214,666 + GFF4844 0.85 -0.1
214,666 + GFF4844 0.85 +0.6
214,666 + GFF4844 0.85 -0.3
214,667 - GFF4844 0.85 +1.4
214,667 - GFF4844 0.85 +0.6
214,667 - GFF4844 0.85 -0.2
214,823 + +0.6
214,824 - -0.1
214,895 + -0.4
214,896 - -0.0
214,919 + -0.3
214,919 + +1.1
214,920 - -0.9
215,243 + GFF4845 0.47 +0.3
215,243 + GFF4845 0.47 -0.7
215,243 + GFF4845 0.47 -0.2
215,244 - GFF4845 0.47 +0.5
215,244 - GFF4845 0.47 +0.4
215,244 - GFF4845 0.47 -0.7
215,244 - GFF4845 0.47 -2.3
215,273 + GFF4845 0.51 +0.8
215,273 + GFF4845 0.51 -0.2
215,273 + GFF4845 0.51 +0.1
215,273 + GFF4845 0.51 +0.5
215,273 + GFF4845 0.51 +0.2
215,274 - GFF4845 0.51 +0.1
215,274 - GFF4845 0.51 -0.8
215,274 - GFF4845 0.51 -0.8
215,274 - GFF4845 0.51 -0.3
215,286 - GFF4845 0.53 -0.9
215,324 + GFF4845 0.58 +0.4

Or see this region's nucleotide sequence