Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4833 and GFF4834 are separated by 151 nucleotides GFF4834 and GFF4835 are separated by 24 nucleotides GFF4835 and GFF4836 are separated by 112 nucleotides GFF4836 and GFF4837 are separated by 41 nucleotides
GFF4833 - Translation initiation factor 3, at 203,827 to 204,372
GFF4833
GFF4834 - LSU ribosomal protein L35p, at 204,524 to 204,727
GFF4834
GFF4835 - LSU ribosomal protein L20p, at 204,752 to 205,111
GFF4835
GFF4836 - Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20), at 205,224 to 206,300
GFF4836
GFF4837 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 206,342 to 208,783
GFF4837
Position (kb)
205
206
207 Strain fitness (log2 ratio)
-2
-1
0
1 at 204.382 kb on + strand at 204.382 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.311 kb on + strand at 206.315 kb on + strand at 206.315 kb on + strand at 206.315 kb on + strand at 206.315 kb on + strand at 206.315 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 204,382 + -0.5 204,382 + -1.1 206,311 + -1.4 206,311 + -0.4 206,311 + -0.4 206,311 + +0.8 206,311 + -0.0 206,311 + -0.4 206,311 + -1.2 206,311 + -0.3 206,311 + -0.5 206,311 + -0.7 206,311 + +0.8 206,311 + -0.1 206,311 + -0.9 206,311 + -0.2 206,311 + -0.6 206,311 + -0.6 206,311 + +1.6 206,311 + -0.3 206,311 + +0.1 206,311 + -0.8 206,311 + -0.6 206,311 + -0.5 206,311 + +1.1 206,311 + +0.5 206,311 + -1.0 206,311 + +0.2 206,311 + +1.3 206,311 + -0.7 206,311 + +0.1 206,311 + +0.5 206,315 + +0.1 206,315 + -0.0 206,315 + -2.4 206,315 + +0.3 206,315 + +0.1
Or see this region's nucleotide sequence