Strain Fitness in Variovorax sp. SCN45 around GFF4835

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4833 and GFF4834 are separated by 151 nucleotidesGFF4834 and GFF4835 are separated by 24 nucleotidesGFF4835 and GFF4836 are separated by 112 nucleotides GFF4833 - Translation initiation factor 3, at 203,827 to 204,372 GFF4833 GFF4834 - LSU ribosomal protein L35p, at 204,524 to 204,727 GFF4834 GFF4835 - LSU ribosomal protein L20p, at 204,752 to 205,111 GFF4835 GFF4836 - Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20), at 205,224 to 206,300 GFF4836 Position (kb) 204 205 206Strain fitness (log2 ratio) -1 0 1at 204.382 kb on + strandat 204.382 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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204,382 + -0.5
204,382 + -1.1

Or see this region's nucleotide sequence