Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4794 and GFF4795 overlap by 4 nucleotides GFF4795 and GFF4796 are separated by 116 nucleotides GFF4796 and GFF4797 are separated by 22 nucleotides GFF4797 and GFF4798 overlap by 4 nucleotides
GFF4794 - Ectoine hydroxylase (EC 1.17.-.-), at 164,528 to 165,403
GFF4794
GFF4795 - predicted biotin regulatory protein BioR (GntR family), at 165,400 to 166,107
GFF4795
GFF4796 - L-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.16), at 166,224 to 167,183
GFF4796
GFF4797 - ABC-type antimicrobial peptide transport system, ATPase component, at 167,206 to 167,856
GFF4797
GFF4798 - tRNA dimethylallyltransferase (EC 2.5.1.75), at 167,853 to 168,890
GFF4798
Position (kb) 166
167
168 Strain fitness (log2 ratio) -1
0
1
2 at 165.289 kb on - strand, within GFF4794 at 165.289 kb on - strand, within GFF4794 at 165.289 kb on - strand, within GFF4794 at 165.357 kb on + strand at 165.357 kb on + strand at 165.357 kb on + strand at 165.358 kb on - strand at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.687 kb on - strand, within GFF4795 at 165.687 kb on - strand, within GFF4795 at 165.687 kb on - strand, within GFF4795 at 165.687 kb on - strand, within GFF4795 at 165.845 kb on + strand, within GFF4795 at 165.846 kb on - strand, within GFF4795 at 166.052 kb on + strand at 166.135 kb on + strand at 166.163 kb on + strand at 166.164 kb on - strand at 166.164 kb on - strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.236 kb on - strand at 166.236 kb on - strand at 166.236 kb on - strand at 166.236 kb on - strand at 166.236 kb on - strand at 166.239 kb on + strand at 166.239 kb on + strand at 166.239 kb on + strand at 166.239 kb on + strand at 166.240 kb on - strand at 166.240 kb on - strand at 166.240 kb on - strand at 166.240 kb on - strand at 166.240 kb on - strand at 166.567 kb on - strand, within GFF4796 at 166.567 kb on - strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.588 kb on - strand, within GFF4796 at 166.588 kb on - strand, within GFF4796 at 166.666 kb on - strand, within GFF4796 at 166.666 kb on - strand, within GFF4796 at 166.666 kb on - strand, within GFF4796 at 166.824 kb on + strand, within GFF4796 at 166.825 kb on - strand, within GFF4796 at 166.944 kb on + strand, within GFF4796 at 166.944 kb on + strand, within GFF4796 at 166.944 kb on + strand, within GFF4796 at 166.944 kb on + strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 167.822 kb on + strand at 167.822 kb on + strand at 167.905 kb on + strand at 167.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 165,289 - GFF4794 0.87 +0.5 165,289 - GFF4794 0.87 -0.7 165,289 - GFF4794 0.87 -1.0 165,357 + +1.0 165,357 + -0.1 165,357 + -1.5 165,358 - -1.3 165,686 + GFF4795 0.40 -0.8 165,686 + GFF4795 0.40 -0.6 165,686 + GFF4795 0.40 -1.3 165,686 + GFF4795 0.40 -0.6 165,686 + GFF4795 0.40 +0.5 165,686 + GFF4795 0.40 -0.2 165,687 - GFF4795 0.41 -0.3 165,687 - GFF4795 0.41 +0.3 165,687 - GFF4795 0.41 -0.6 165,687 - GFF4795 0.41 +0.5 165,845 + GFF4795 0.63 -0.3 165,846 - GFF4795 0.63 -0.0 166,052 + +0.0 166,135 + +0.2 166,163 + -1.3 166,164 - -0.4 166,164 - +0.6 166,231 + +0.4 166,231 + +0.9 166,231 + +1.0 166,231 + +2.3 166,231 + -0.8 166,231 + +0.5 166,231 + +0.1 166,231 + +0.5 166,231 + -0.3 166,232 - +0.0 166,232 - -0.5 166,232 - -0.9 166,232 - -1.0 166,232 - -1.5 166,232 - -0.1 166,232 - +0.4 166,232 - -0.8 166,232 - +0.4 166,232 - +0.0 166,232 - -0.4 166,232 - +0.2 166,232 - -0.2 166,232 - +1.3 166,235 + -1.0 166,235 + +0.2 166,235 + +0.4 166,235 + +0.3 166,235 + +0.2 166,235 + +0.4 166,236 - -0.4 166,236 - -1.0 166,236 - +0.9 166,236 - +0.8 166,236 - -1.2 166,239 + -0.3 166,239 + +1.9 166,239 + -1.0 166,239 + -0.6 166,240 - -0.5 166,240 - -0.1 166,240 - +0.7 166,240 - -1.2 166,240 - +0.5 166,567 - GFF4796 0.36 -0.4 166,567 - GFF4796 0.36 -0.3 166,587 + GFF4796 0.38 -0.7 166,587 + GFF4796 0.38 -0.3 166,587 + GFF4796 0.38 -1.7 166,587 + GFF4796 0.38 -0.5 166,587 + GFF4796 0.38 -1.3 166,588 - GFF4796 0.38 +0.2 166,588 - GFF4796 0.38 +0.1 166,666 - GFF4796 0.46 -0.0 166,666 - GFF4796 0.46 +0.0 166,666 - GFF4796 0.46 +0.1 166,824 + GFF4796 0.62 -0.8 166,825 - GFF4796 0.63 +0.4 166,944 + GFF4796 0.75 -0.3 166,944 + GFF4796 0.75 -0.3 166,944 + GFF4796 0.75 -1.2 166,944 + GFF4796 0.75 -0.0 166,945 - GFF4796 0.75 -1.4 166,945 - GFF4796 0.75 -0.5 166,945 - GFF4796 0.75 -1.0 166,945 - GFF4796 0.75 +0.6 166,945 - GFF4796 0.75 -0.6 167,822 + -0.6 167,822 + -1.2 167,905 + -1.7 167,906 - -0.5
Or see this region's nucleotide sequence